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Adaptation of bacteria to toxic solvents [Elektronische Ressource] : mechanisms and application / vorgelegt von Grit Neumann

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157 pages
Adaptation of bacteria to toxic solvents: mechanisms and application I n a u g u r a l d i s s e r t a t i o n zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald vorgelegt von Grit Neumann geboren am 02.09.1977 in Teterow Greifswald, Juli 2006 Dekan: Prof. Dr. rer. nat. Klaus Fesser 1. Gutachter: Prof. Dr. rer. nat. Frieder Schauer 2. Gutachter: Prof. Dr. rer. nat. Hauke Harms Tag der Promotion: 24.11.2006 Für meine Famile Whatever we know is only a drop. What we don't know is an ocean. (Sir Isaac Newton) CONTENTS 1 INTRODUCTION................................................................................................. 1 2 CELLS OF PSEUDOMONAS PUTIDA AND ENTEROBACTER SP. ADAPT TO TOXIC ORGANIC COMPOUNDS BY INCREASING THEIR SIZE................... 47 3 THE REGULATION OF THE CIS-TRANS ISOMERASE (CTI) OF UNSATURATED FATTY ACIDS IN PSEUDOMONAS PUTIDA: +CORRELATION BETWEEN CTI ACTIVITY AND K -UPTAKE SYSTEMS......
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Adaptation of bacteria to toxic solvents:
mechanisms and application




I n a u g u r a l d i s s e r t a t i o n
zur
Erlangung des akademischen Grades
doctor rerum naturalium (Dr. rer. nat.)
an der Mathematisch-Naturwissenschaftlichen Fakultät
der
Ernst-Moritz-Arndt-Universität Greifswald






vorgelegt von
Grit Neumann
geboren am 02.09.1977
in Teterow




Greifswald, Juli 2006
























Dekan: Prof. Dr. rer. nat. Klaus Fesser


1. Gutachter: Prof. Dr. rer. nat. Frieder Schauer

2. Gutachter: Prof. Dr. rer. nat. Hauke Harms


Tag der Promotion: 24.11.2006

































Für meine Famile



































































Whatever we know is only a drop.
What we don't know is an ocean. (Sir Isaac Newton)


CONTENTS
1 INTRODUCTION................................................................................................. 1
2 CELLS OF PSEUDOMONAS PUTIDA AND ENTEROBACTER SP. ADAPT TO
TOXIC ORGANIC COMPOUNDS BY INCREASING THEIR SIZE................... 47
3 THE REGULATION OF THE CIS-TRANS ISOMERASE (CTI) OF
UNSATURATED FATTY ACIDS IN PSEUDOMONAS PUTIDA:
+CORRELATION BETWEEN CTI ACTIVITY AND K -UPTAKE SYSTEMS...... 59
4 PREDICTION OF THE ADAPTABILITY OF PSEUDOMONAS PUTIDA DOT-
T1E TO A SECOND PHASE OF A SOLVENT FOR ECONOMICALLY SOUND
TWO-PHASE BIOTRANSFORMATIONS......................................................... 69
5 ENERGETICS AND SURFACE PROPERTIES OF PSEUDOMONAS PUTIDA
DOT-T1E IN A TWO-PHASE FERMENTATION SYSTEM WITH 1-DECANOL
AS SECOND PHASE........................................................................................ 85
6 CONCLUDING REMARKS............................................................................. 101
7 SUMMARY...................................................................................................... 103
8 ZUSAMMENFASSUNG .................................................................................. 105
9 REFERENCES................................................................................................ 107








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