Angiogenesis in the New Zealand obese mouse model fed with high fat diet

Balwierz Adriana, Polus Anna, Razny Urszula, Wator Lukasz, Dyduch Grzegorz, Tomaszewska Romana, Scherneck Stephan, Joost Hans, Dembinska-Kiec, Dembinska-Kiec Aldona

Description

Obesity and its complications lead to vascular injury, atherosclerosis, diabetes and pathological angiogenesis. One of the models to study the obesity and its entanglements is the New Zealand Obese mice model. Aim of this study was to check the effect of high fat diet on changes in biochemical parameters as well as on process of angiogenesis in NZO mice. Methods NZO mice were fed with standard (ST) or high fat (HF) diet for seven weeks. Body weight and serum biochemical parameters were monitored. The PECAM1 positive vessel-like structures immunostaining, as well as the gene expression of the matrigel penetrating cells by microarray (confirmed by real-time PCR method) were analyzed. Results Mice fed with HF diet developed obesity. Number of newly created vessels with lumen was correlated with hyperglycemia and animal weight gain. The number of PECAM1 positive cells in matrigel tended to increase during HF diet. Microarray results revealed changes in gene expression (activation of the oxidative stress and insulin resistance, inhibition of apoptosis and cell differentiation), however without markers of endothelial cell network maturation. Conclusion Observed changes in the NZO mice on HF diet argue for the hyperglycemia related activation of angiogenesis, leading to the formation of pathological, immature network.

Informations

• Publié par: biomed
• Publié le: 01 janvier 2009
• Nombre de lectures: 3
• Langue: English

Extrait

Lipids in Health and Disease

BioMedCentral

Open Access Research Angiogenesis in the New Zealand obese mouse model fed with high fat diet 1,2 11 1 Adriana Balwierz*, Anna Polus, Urszula Razny, Lukasz Wator, 3 34 4 Grzegorz Dyduch, RomanaTomaszewska ,Stephan Scherneck, HansJoost 1 and Aldona DembinskaKiec

1 2 Address: Departmentof Clinical Biochemistry, Collegium Medicum, Jagiellonian University, Cracow, Poland,Postgraduate School of Molecular 3 Medicine, 61 Żwirki i Wigury Str., 02091 Warsaw, Poland,Department of Pathomorphology, Collegium Medicum, Jagiellonian 4 University, Cracow, Poland andGerman Institute of Human Nutrition, PotsdamRehbrücke, Germany Email: Adriana Balwierz*  abalwierz@cmuj.krakow.pl; Anna Polus  apolus@cmuj.krakow.pl; Urszula Razny  urazny@cmuj.krakow.pl; Lukasz Wator  [e-mail]; Grzegorz Dyduch  dyduch@cmuj.krakow.pl; Romana Tomaszewska  tomasz@cmuj.krakow.pl; Stephan Scherneck  [e-mail]; Hans Joost  [e-mail]; Aldona DembinskaKiec  mbkiec@cyfkr.edu.pl * Corresponding author

Published: 6 April 2009Received: 26 January 2009 Accepted: 6 April 2009 Lipids in Health and Disease2009,8:13 doi:10.1186/1476511X813 This article is available from: http://www.lipidworld.com/content/8/1/13 © 2009 Balwierz et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:Obesity and its complications lead to vascular injury, atherosclerosis, diabetes and pathological angiogenesis. One of the models to study the obesity and its entanglements is the New Zealand Obese mice model. Aim of this study was to check the effect of high fat diet on changes in biochemical parameters as well as on process of angiogenesis in NZO mice. Methods:NZO mice were fed with standard (ST) or high fat (HF) diet for seven weeks. Body weight and serum biochemical parameters were monitored. The PECAM1 positive vessellike structures immunostaining, as well as the gene expression of the matrigel penetrating cells by microarray (confirmed by realtime PCR method) were analyzed. Results:Mice fed with HF diet developed obesity. Number of newly created vessels with lumen was correlated with hyperglycemia and animal weight gain. The number of PECAM1 positive cells in matrigel tended to increase during HF diet. Microarray results revealed changes in gene expression (activation of the oxidative stress and insulin resistance, inhibition of apoptosis and cell differentiation), however without markers of endothelial cell network maturation. Conclusion:Observed changes in the NZO mice on HF diet argue for the hyperglycemia related activation of angiogenesis, leading to the formation of pathological, immature network.

Introduction Obesity, insulin resistance (hyperinsulinemia, hyperglyc emia), dyslipidemia (hypertriglyceridemia with low blood HDL levels) hyperleptinemia and hypoadi ponectinemia, elevated biochemical parameters of inflammatory response, as well as activation of coagula tion cascade are main features of metabolic syndrome

leading to micro and macrovascular injury, hypertension, and atherosclerosis [1,2]. These events impair vessel func tions and lead to diabetic retinopathy, which is a major cause of blindness in industrialized countries [3].

High fat (HF) diet promotes progression of impaired glu cose tolerance, induces insulin resistance and with

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