Central catecholamine depletion inhibits peripheral lymphocyte proliferation and splenocyte cytokine production [Elektronische Ressource] / vorgelegt von Wei Kou

Universitäts Klinikum Essen

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Medizinische Fakultät der Universität Duisburg-Essen Aus dem Institut für Medizinische Psychologie Central catecholamine depletion inhibits peripheral lymphocyte proliferation and splenocyte cytokine production Inaugural–Dissertation zur Erlangung des Doktorgrades der Medizin durch die Medizinische Fakultät der Universität Duisburg-Essen Vorgelegt von Wei Kou aus Tanggu, Tianjin, China 2004 Medical Faculty of the University of Duisburg-Essen Department of Medical Psychology Central catecholamine depletion inhibits peripheral lymphocyte proliferation and splenocyte cytokine production Inaugural-Dissertation For Application for Doctor’s Degree in Medicine in the Medical Faculty of the University of Duisburg-Essen Presented by Wei Kou From Tianjin, P.R. China 2004 Dekan: Univ.-Prof. Dr. med. H. Grosse-Wilde 1. Gutachter: Univ.-Prof. Dr. rer. biol. hum. Dipl.-Psych. M. Schedlowski 2. Gutachter: Prof. Dr. rer. nat. O.-E. Brodde Tag der mündlichen Prüfung: 30. August 2004 - 2 -Contents 1. Introduction 1.1 Overview 51.2 Occurrence and regional distribution of catecholamines in the central nervous system 61.3Central catecholamines and immunity 111.3.1Correlation between central catecholamines and immunity in pathophysiological conditions 111.3.

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• Gesundheit

Informations

• Publié par: universitat_duisburg-essen
• Publié le: 01 janvier 2004
• Nombre de lectures: 31

Extrait

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Medizinische Fakultät der Universität Duisburg-Essen

Aus dem Institut für Medizinische Psychologie

Central catecholamine depletion inhibits peripheral lymphocyte proliferation and splenocyte cytokine production

I n a u g u r a l – D i s s e r t a t i o n zur Erlangung des Doktorgrades der Medizin durch die Medizinische Fakultät der Universität Duisburg-Essen          Vorgelegt von Wei Kou aus Tanggu, Tianjin, China 2004

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  Medical Faculty of the University of Duisburg-Essen

Department of Medical Psychology

       Central catecholamine depletion inhibits peripheral lymphocyte proliferation and splenocyte cytokine production        

Inaugural-Dissertation  For Application for Doctors Degree in Medicine in the Medical Faculty of the University of Duisburg-Essen     Presented by Wei Kou From Tianjin, P.R. China 2004

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  Dekan:  Univ.-Prof. Dr. med. H. Grosse-Wilde 1. Gutachter: Univ.-Prof. Dr. rer. biol. hum. Dipl.-Psych. M. Schedlowski 2. Gutachter: Prof. Dr. rer. nat. O.-E. Brodde 

Tag der mündlichen Prüfung: 30. August 2004

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Contents 

1.Introduction  1.1  Overview 1.2  Occurrence and regional distribution of catecholamines in the central nervous system 1.3  Central catecholamines and immunity 1.3.1  Correlation between central catecholamines and immunity in pathophysiological conditions 1.3.2  Pharmacological manipulation of central catecholamines and immunity 1.4  Aim of the study 2.  Material and Methods  2.1  Animals 2.2  Experimental design 2.3  Central catecholamine depletion 2.4  Catecholamine determination 2.5  Splenocytes preparation 2.6  ConA-induced splenocyte proliferation 2.7  Peripheral lymphocyte preparation 2.8  ConA-induced peripheral lymphocyte proliferation 2.9  ELISA detection for IL-2 and IFN-γin splenocyte proliferation supernatant 2.9.1  ELISA detection for IL-2 in splenocyte proliferation supernatant 2.9.2  ELISA detection for IFN-γin splenocyte proliferation supernatant 2.10 RT-PCR analysis of IL-2 mRNA expression 2.10.1 Total RNA isolation 2.10.2 Reverse transcription 2.10.3 Amplification of specific complementary DNA 2.11 Statistical analysis 3.  Results  3.1  Effects of i.c.v. 6-OHDA treatment on central and peripheral catecholamine concentrations

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3.2Effects of central catecholamine depletion on lymphocyte proliferation 3.3  Effects of central catecholamine depletion on splenocyte cytokine production 3.4  Effects of central catecholamine depletion on splenocyte IL-2 mRNA expression

4.Discussion

5.ioanetvibrAsb

6.Summary

7.References

8.Acknowlegements

9.Curriculum vitae

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1.Introduction  1.1  Overview  Traditionally, the nervous and immune systems have been regarded to function largely independently of each other. Evidence accumulated during the past two decades, however, has demonstrated the bidirectional communications between the central nervous system (CNS) and the immune system.  The first sustained program of research on brain-immune system interactions began in the 1920s and concerned the Pavlovian conditioning of immune reactions. In the 1950s, there was a short-lived, but later received, interest in the immunologic effects of lesioning and electrical stimulation of the hypothelamus. At the same time, Fred Rasmussen, Jr., a virologist, and Norma Brill, a psychiatrist, initiated studies on the effects of stressful life experiences on susceptibility to experimentally induced infectious diseases (Ader et al., 2001).  Interest in this interdisciplinary research was rejuvenated when, in the 1970s, several independent lines of research converged to provide verifiable evidence of interactions between the brain and the immune system. John Hadden linked the immune system to the sympathetic nervous system (SNS) by documenting the existence ofβ-cigrenerda receptors (ARs) on lymphocytes (Hadden et al., 1970); Robert Ader and Nicholas Cohen demonstrated behaviorally conditioned suppression of the immune system, providing a functional link between the brain and the immune system (Ader and Cohen, 1975); Roger Bartrop and his associates described immunologic changes associated with the bereavement that following the death of a spouse (Bartrop et al., 1977); Hugo Besedovsky began to piece together a neuroendocrine-immune system network with his studies of the effects of immune responses on neural and endocrine functions (Besedovsky et al., 1983); the sympathetic innervation of lymphoid tissues was documented by David and Suzanne Felten (Felten et al., 1987a; Felten and Olschowka, 1987) and connections to the thymus were documented by Karen Bulloch (Bulloch and Pomerantz, 1984); and Edwin Blalock and Eric Smith showed that lymphocytes, themselves, were capable of producing neuropeptides (Blalock and Smith, 1980). These seminal studies precipitated new research on the receptors for hormones and neurotransmitters that existed on lymphocytes, the

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