Cet ouvrage et des milliers d'autres font partie de la bibliothèque YouScribe
Obtenez un accès à la bibliothèque pour les lire en ligne
En savoir plus

Partagez cette publication

Isabelleet al.Proteome Science2010,8:22 http://www.proteomesci.com/content/8/1/22
R E S E A R C HOpen Access Research Investigation of PARP-1, PARP-2, and PARG interactomes by affinity-purification mass spectrometry
1 11,2 11 1 Maxim Isabelle, Xavier Moreel, Jean-Philippe Gagné, Michèle Rouleau, Chantal Ethier, Pierre Gagné, 3 1,4 Michael J Hendzeland Guy G Poirier*
Background14, -15, and -16) or catalytically inactive members (PARP-Poly(ADP-ribose) polymerases (PARPs) catalyze the for-9 and -13). Poly(ADP-ribose) glycohydrolase (PARG), on mation of poly(ADP-ribose) (pADPr), a protein post-the other hand, catabolizes pADPr and thereby accounts translational modification involved in several importantfor the transient nature of poly(ADP-ribosyl)ation. biological processes, namely surveillance of genomeIn this study, we chose to investigate PARP-1 and integrity, cell cycle progression, initiation of the DNAPARP-2 because of their pivotal role in the maintenance damage response, apoptosis, and regulation of transcrip-of genome integrity, and PARG to cover both the synthe-tion (reviewed in [1]). Recently Kleineet al.sis and degradation components of pADPr metabolism.[2] limited the PARP family to PARPs possessing the HYE catalyticThrough their strand break-dependent PARP activity, core motif as well as a long β4/β5 loop, namely PARP-1, -both PARP-1 and PARP-2 are able to initiate a rapid 2, -3, tankyrase-1 and -2, and vault-PARP. All other puta-response to DNA damage via pADPr synthesis on them-tive PARP family members were re-classified as mono-selves (automodification) and on other nuclear acceptors ADP-ribosyltransferases (PARP-6, -7, -8, -10, -11, -12, -such as histones. This DNA damage response facilitates base-excision repair (BER) [3,4] and contributes to non-* Correspondence: guy.poirier@crchul.ulaval.ca 1homologous end joining (NHEJ) [5,6]. Empirical evidence Axe cancer, CHUQ Research Center, Faculty of Medicine, Laval University, 2705 Boulevard Laurier, Québec, Canada, G1V 4G2however indicates that the functions of PARP-2 do not Full list of author information is available at the end of the article © 2010 Isabelle et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons BioMedCentral Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Un pour Un
Permettre à tous d'accéder à la lecture
Pour chaque accès à la bibliothèque, YouScribe donne un accès à une personne dans le besoin