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Optimization and characterization of microbial rhamnolipid production from renewable resources [Elektronische Ressource] / Markus Michael Müller. Betreuer: C. Syldatk

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144 pages
Ajouté le : 01 janvier 2011
Lecture(s) : 30
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Optimization and characterization of
microbial rhamnolipid production from
renewable resources


Zur Erlangung des akademischen Grades eines
DOKTORS DER INGENIEURWISSENSCHAFTEN (Dr.-Ing.)

von der Fakultät für Chemieingenieurwesen und Verfahrenstechnik
des Karlsruher Instituts für Technologie (KIT)


genehmigte DISSERTATION


von
Dipl.-Biotechnol. Markus Michael Müller
aus Heidenheim an der Brenz




Referent: Prof. Dr. rer. nat. Christoph Syldatk
Korreferent: Prof. Dr.-Ing. Clemens Posten
Tag der mündlichen Prüfung: 23.09.2011 Acknowledgements

I would like to thank the following persons and organisations for their support during
my doctoral thesis:
Prof. Dr. rer. nat. C. Syldatk I would like to thank for giving me the possibility to carry
out my doctoral thesis in his working group, for his guidance and support and for the
academic freedom and interesting discussions. Additionally, I want to express
gratefulness to PD Dr.-Ing. Rudolf Hausmann for his supervision and for always
finding some of his rare time to discuss the state of the science.
Prof. Dr.-Ing. C. Posten I would like to thank for the friendly acceptance of the co-
referee.
I want to thank all friends and colleagues at the Technical Biology for their company
and the friendly atmosphere in the institute and for after-work activities. Special
thanks go to the members of the DOx, FAB and FAK groups, Sandra Baumann,
Melanie Gerlitzki, Markus Andre, Mareike Perzborn, Frank Ströhle and Marius Henkel
from the Technical Biology. Additionally, I want to thank Anke Schmidberger, Ingo
Fischer, Tobias Müller and Martin Silvestre from KIT Campus Nord.
I would also like to thank the students and guest researchers who contributed either
active or by discussion to the development of this work, Nadine Tritsch, Stefan
Merkle, Josia Assmies, Florian Bär, Roberta Lovaglio, Roberta Malavenda, Hossein
Amani and Saleh Matar, for the experimental input from their research projects or
diploma thesis, for the shared hours in the lab and “Technikum” and for giving me the
opportunity to teach and learn. Siegfried Almstedt for construction and installation of
a new non-commercially available mechanical foam breaker, Michaela Zwick for her
support and input to technical and fermentation issues, and Barbara Hörmann for her
contribution to the rhamnolipid project and the pleasant lab atmosphere.
Our academic cooperation partners from the Fraunhofer IOSB, KIT Campus Nord, FZ
Jülich, TU Braunschweig and the University of Ulm, as well as all industrial
cooperation partners. The Fachagentur Nachwachsende Rohstoffe e.V. (FNR) for the
financial support of the project „Optimierung der Herstellung mikrobieller
Rhamnolipide auf Basis nachwachsender Rohstoffe“.

I
II Preamble

The thesis at hand is based on a compendium of peer-reviewed works that were
independently published during the last three years. These publications compose of
one general introducing book chapter, two original papers which reflect a part of the
laboratory work, and a review article that is concluding the last years of rhamnolipid
research and gives an outlook of the possible and necessary works for the future.
Chapter 1 is a general introduction and was published from PanStanford Publishing
Pte. Ltd. (New Jersey, London, Singapore) in 2011 as “Chapter 16: Microbial
rhamnolipids” in “Carbohydrate-Modifying Biocatalysts” which was edited by
Prof. Dr. P. Grunwald of the Hamburg-Harburg University.
The Chapters 2 and 3 reflect a principal part of the laboratory work performed during
the last three years. The main problems encountered with Pseudomonas aeruginosa
during rhamnolipid bioreactor production are presented and discussed. Chapter 2
was published as “Pseudomonas aeruginosa PAO1 as a model for rhamnolipid
production in bioreactor systems” in 2010 and Chapter 3 as “Evaluation of
rhamnolipid production capacity of Pseudomonas aeruginosa PAO1 in comparison to
the rhamnolipid over-producer strains DSM 7108 and DSM 2874” in 2011, both in the
Applied Microbiology and Biotechnology Journal from Springer (Heidelberg).
Finally, in Chapter 4 the main conclusions and encountered problems of the last 60
years of rhamnolipid research were put into this Mini-Review which will be published
as “Regulatory and metabolic network of rhamnolipid biosynthesis: Traditional and
advanced engineering towards biotechnological production” in 2011 in the Applied
Microbiology and Biotechnology Journal from Springer (Heidelberg).
The presented publications are identical in content but not in layout with the
published versions. If necessary, urgent changes were made and highlighted by
footmarks.

III Publications and presentations
Original papers and reviews
2011 Müller MM and Hausmann R.
Regulatory and metabolic network of rhamnolipid biosynthesis:
Traditional and advanced engineering towards biotechnological
production.
Applied Microbiology and Biotechnology;
(DOI) 10.1007/s00253-011-3368-2

2011 Müller MM, Hörmann B, Kugel M, Syldatk C and Hausmann R.
Evaluation of rhamnolipid production capacity of Pseudomonas
aeruginosa PAO1 in comparison to the rhamnolipid over-
producer strains DSM 7108 and DSM 2874.
Applied Microbiology and Biotechnology; 89; (3): 585-592

2010 Hörmann B, Müller MM, Syldatk C and Hausmann R.
TRhamnolipid production by Burkholderia plantarii DSM 9509 .
European Journal of Lipid Science and Technology; 112; 674-
680.

2010 Müller MM, Hörmann B, Syldatk C and Hausmann R.
Pseudomonas aeruginosa PAO1 as a model for rhamnolipid
production in bioreactor systems.
Applied Microbiology and Biotechnology. 87; (1): 167-174.

2009 Martinez JC, Müller MM, Turley H, Steer G, Choteau L, Li J-L.
Saison R, Harris AL, Pezzella F and Gatter KC.
Nuclear and membrane expression of the angiogenesis regulator
delta-like 4 (DLL4) in normal and malignant human tissues.
Histopathology; 54; 598-606.

Book chapters
2011 Müller MM, Hörmann B, Syldatk C and Hausmann R.
Microbial Rhamnolipids IN Carbohydrate Modifying Biocatalysts
Prof. Peter Grunwald (ed.), Pan Stanford Publishing Pte Ltd.
ISBN-10: 981-4-241-679 in press

2011 Abdel-Mawgoud AM, Hausmann R, Lépine F, Müller MM and
Déziel E.
Biosurfactants: From genes to applications IN Microbiology
Monographs series Vol. 20 Gloria Soberón-Chávez (ed.),
Springer.
ISBN: 978-3-642-14489-9

IV Talks and presentations at conferences
Sept. 2011 Talk “Rhamnolipids: Next Generation Biosurfactants”
1st European Congress of Applied Biotechnology in Berlin
(announced)

April 2011 Talk “Rhamnolipids: Green surfactants based on renewables”
VAAM Annual Meeting in Karlsruhe

Sept. 2010 Poster presentation
28. Annual Meeting of DECHEMA in Aachen

May 2010 Poster presentation
DECHEMA Symposium: “Bioprozessorientiertes Anlagendesign“
in Nürnberg

Sept. 2009 Poster presentation
27. Annual Meeting of DECHEMA in Mannheim

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