Quasi-MSnidentification of flavanone 7-glycoside isomers in Da Chengqi Tangby high performance liquid chromatography-tandem mass spectrometry

Xu Fengguo, Liu Ying, Zhang Zunjian, Yang Cheng, Tian, Tian Yuan

Description

Da Chengqi Tang (DCT) is a common purgative formula in Chinese medicine. Flavanones are its major active compounds derived from Fructus Aurantii Immaturus . The present study developed an LC-MS/MS method to characterize two pairs of flavanone 7-glycoside isomers, i.e., hesperidin versus neohesperidin and naringin versus isonaringin. Methods After solid phase purification, components in sample were separated on a Agilent zorbax SB-C18 (5 μm, 250 mm × 4.6 mm) analytical column. ESI-MS and quasi-MS n were performed in negative ion mode to obtain structural data of these two pairs of flavanone 7-glycoside isomers. Moreover, UV absorption was measured. Results There was no intra-pairs difference in the UV-Vis and MS/MS spectra of the two pairs of 7-glycoside isomers, whereas the mass spectrometry fragmentation pathways between pairs were different. Conclusion The present study developed a LC-MS/MS method to explore the inter- and intra-pair difference of two pairs of flavanone 7-glycoside isomers.

Informations

• Publié par: biomed
• Publié le: 01 janvier 2009
• Nombre de lectures: 0
• Langue: English

Extrait

Chinese Medicine

BioMedCentral

Open Access Research n Quasi-MS identificationof flavanone 7-glycoside isomers inDa Chengqi Tangby high performance liquid chromatography-tandem mass spectrometry 1,2 31,2 1,21,2 Fengguo Xu, Ying Liu, Zunjian Zhang*, Cheng Yangand Yuan Tian

1 Address: KeyLaboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 2 Jiangsu 210009, PR China,Center for Instrumental Analysis, China Pharmaceutical University, Nanjing, Jiangsu 210009, PR China and 3 Department of Pharmacy, Nanjing Municipal Hospital of Traditional Chinese Medicine, Nanjing, Jiangsu 210001, PR China Email: Fengguo Xu  [e-mail]; Ying Liu  [e-mail]; Zunjian Zhang*  [e-mail]; Cheng Yang  [e-mail]; Yuan Tian  [e-mail] * Corresponding author

Published: 24 July 2009Received: 20 December 2008 Accepted: 24 July 2009 Chinese Medicine2009,4:15 doi:10.1186/1749-8546-4-15 This article is available from: http://www.cmjournal.org/content/4/1/15 © 2009 Xu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:Da Chengqi Tang(DCT) is a common purgative formula in Chinese medicine. Flavanones are its major active compounds derived fromFructus Aurantii Immaturus. The present study developed an LC-MS/MS method to characterize two pairs of flavanone 7-glycoside isomers, i.e., hesperidin versus neohesperidin and naringin versus isonaringin. Methods:After solid phase purification, components in sample were separated on a Agilent n zorbax SB-C18 (5μm, 250 mm × 4.6 mm) analytical column. ESI-MS and quasi-MSwere performed in negative ion mode to obtain structural data of these two pairs of flavanone 7-glycoside isomers. Moreover, UV absorption was measured. Results:There was no intra-pairs difference in the UV-Vis and MS/MS spectra of the two pairs of 7-glycoside isomers, whereas the mass spectrometry fragmentation pathways between pairs were different. Conclusion:The present study developed a LC-MS/MS method to explore the inter- and intra- pair difference of two pairs of flavanone 7-glycoside isomers.

Background Described inShanghan Lun(Treatise on Cold Damage Dis eases, a Chinese medicine classic from the Han dynasty) [1],Da Chengqi Tang(DCT) is a well known purgative for mula consisting ofRadix et Rhizoma Rhei(Dahuang),Cor tex Magnoliae officinalis(Houpu),Fructus Aurantii Immaturus(Zhishi) andNatrii Sulfas(Mangxiao). DCT is usually used to treat diseases such as acute intestinal obstruction without complications, acute cholecystitis and appendicitis [2]. DCT is also used in treating posttrau

matic respiratory distress syndrome [3], reducing acute phase protein levels in patients with multiple organ fail ure syndromes [4] and relieving inflammation in patients after tumor operation [5]. Recently DCT was found to pos sess antiinflammatory effects apart from its purgative activities [6].

Flavanones are a major type of active components in DCT [1,6]. Various studies have revealed a variety of pharmaco logical activities that citrus flavanones possess, such as

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