COST Action 97

European Commission Directorate-General For Research And Innovation

Description

Pathogenic micro-organisms in poultry and eggs: Monitoring procedures, rapid detection methods and techniques: Proceedings of a workshop held in the Institute for Animal Health, Compton, Newbury, United Kingdom, 21 to 23 April 1996
Veterinary sector and animal health

Sujets

• Science and Technology Policy Research
• COST ? European Cooperation in the Field of Scientific and Technical Research

Informations

• Publié par: EUROPEAN-COMMISSION-DIRECTORATE-GENERAL-FOR-RESEARCH-AND-INNOVATION
• Nombre de lectures: 11
• Langue: English
• Poids de l'ouvrage: 7 Mo

Extrait

Pathogenic micro-organisms in poultry and eggs
2. Monitoring procedures, rapid
detection methods and
techniques
Edited
C.J.Thoms and P.Jones
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Compton, Newbury, United Kingdom, 21 to 23 April 1996
EUROPEAN COMMISSION EUROPEAN COMMISSION
COST Action 97
Pathogenic micro-organisms in poultry and eggs
2. Monitoring procedures, rapid
detection methods and techniques
Working Group 4
Proceedings of a workshop held in the Institute for Animal Health, Compton,
Newbury, United Kingdom, 21 to 23 April 1996
Edited by
C. J. Thorns
Central Veterinary Laboratory,
Addlestone
United Kingdom
P. Jones
Institute for Animal Health,
Compton, United Kingdom A great deal of additional information on the European Union is available on the Internet.
It can be accessed through the Europa server (http://europa.eu.int)
Cataloguing data can be found at the end of this publication
Luxembourg: Office for Official Publications of the European Communities, 1997
ISBN 92-827-9885-2
© European Communities, 1997
Reproduction is authorized, except for commercial purposes, provided the source is
acknowledged
Printed in the United Kingdom CONTENTS
INTRODUCTION 5
Ζ. Somogyi (Η) — Introduction to COST co-operation 7
S. Notermans, A. Van de Giessen and A. M. Henken (NL) — Future requirements for
diagnosing and monitoring of pathogenic micro-organisms in poultry and eggs 9
T. Humphrey (UK) — Methods for the isolation of pathogens from poultry and poultry
products 15
H. Ibrahim Atabay and J. E. L. Corry (UK) — Use of novel techniques for the
isolation of unusual Campylobacters from cattle and poultry 26
D. De Medici, L. Croci, G. Ciccaglioni, G. G. Costamagna and L. Toti (I) —
Effectiveness of the modified semi-solid Rappaport-Vassiliadis medium (MRSV) for the
detection of salmonella in frozen meat products 3
C. Wray, R. H. Davies and I. M. McLaren (UK) — Rapid: How rapid? Personal
reflections on salmonella isolation 40
P. Barrow (UK) — Serological diagnosis of Salmonella infections in poultry by ELISA
with particular reference to S. enteritidis7
A. C. Pinder, R. G. Clarke and D. Cunliffe (UK) — Rapid assay for pathogenic
salmonella organisms in eggs and dairy products using flow cytometry and fluorescent
monoclonal antibodies 60
R. H. Davies and C. Wray (UK) — Development of a rapid test unit for salmonella....6
F. Sisak, M. Faldynova and L. Dedek (CR) — Serological test ELISA for the detection
of Salmonella enteritidis and Salmonella typhimurium infections in poultry 72
R. Helmuth, W. Rabisch and D. Protz (D) — Novel nucleic acid-based techniques and
their application to pathogenic micro-organisms in poultry and eggs 79
D. G. Newell, R. A. Ayling and S. Evans (UK) — The application of PCR/RFLP to the
typing of Campylobacters isolated from poultry in the UK 85
J. E. Olsen, S. Aabo and L. Rossen (DK) — The development of oligonucleotide probes
and PCR methods specific for salmonella or for salmonella serotype typhimurium and
application to detection in naturally contaminated samples 92 C. Soumet, E. Gwennola and P. Colin (F) — PCR for the detection of salmonella genus
in chicken and beef products 99
J. H. J. Huis in't Veld and H. V. D. Zee (NL) — Instrumental analysis of food-
associated micro-organisms 107
D. Clarke, M. Harrison, H. Aojula, B. Chapman and S. Johal (UK) — Scope for an
preliminary development of novel in situ immunodetection of foodborne pathogens .... 118
J. Ward, M. Griffin and J. Egan (IRL) — Evaluation of some rapid methods for the
detection of salmonella in poultry carcasses, feed and environmental samples 123
C. J. Thorns (UK) — Summary working group session 1 on diagnostic tests: From
research to routine use: success of failure? 128
S. Notermans (NL) — Summary working group session 2 on: Harmonisation and quality
control of detection techniques 130 INTRODUCTION
The COST Action 97 "Pathogenic Micro-organisms in Poultry and Eggs" continued its
activities with the organisation of a third workshop on: Development of monitoring
procedures, rapid detection methods and techniques. This is one of the four areas of
research in which the Action was divided and organised into Working Groups. The
Chairman of this Working Group is Dr C. Thorns, Central Veterinary Laboratory,
Addlestone, UK, who was also a member of the organising committee
After the Veterinary Medical Institute in Budapest. Hungary and the Veterinary Faculty of
the University of Zaragoza, Spain it was now the Institute for Animal Health, Compton,
UK who hosted the meeting. Participants from 14 European countries attended the
meeting and discussed the state of the art with respect to conventional and novel
microbiological detection and identification methods.
Participants took the opportunity to discuss future co-operation with or without submitting
projects to the European Union's research programme. A special session was included to
update participants on the recent BSE issues associated with meat.
Many thanks to Dr P. Jones (Compton, UK) and all the other local organisers for making it
a stimulating and enjoyable workshop. We would also like to acknowledge the support of
the European Commission, Directorate General XII/BSI (Dr Ζ. Somogyi), Unipath Ltd
and Hoechst UK Ltd.
Dr R.W.A.W. Mulder
Chairman