Seroprevalence of Toxoplasma gondiiinfection among horses in Tunisia

Boughattas Sonia, Bergaoui Ramzi, Essid Rym, Aoun Karim, Bouratbine, Bouratbine Aida

Description

The present study was conducted to investigate the serological survey of Toxoplasma antibodies in local.horses from three major regions: a neighbourhood of a city in the North (Sidi Thabet), a neighbourhood of a city on the coast (Monastir) and a neighbourhood of a city in the middle (Battan) of Tunisia (North of Africa). Methods A total of 158 serum samples were obtained from clinically healthy horses which consisted of 111 (32 female, 79 male) 2-10 years old and 47 (11 female, 36 male) older than 10 years. All of the horses were tested for antibodies to T. gondii using the Modified Agglutination Test (MAT). Results According to MAT results, antibodies to T. gondii were found in 28 (17.7%) of 158 sera with the titers of 1:20 in 20 horses, 1:40 in 1 horse, 1:80 in 2 horses, 1:160 in 2 horses, 1:320 in 1 horse and ≥1:640 in 2 horses. Anti- T. gondii antibodies were found in 18 (16.2%) of 111 horses (2-10 years old) and 10 (21.2%) of 47 horses (older than 10 years old). Six (13.9%) out of 43 female had anti-toxoplasma antibodies and 22 (19.1%) from 115 males remained positive. Conclusion Statistically significant differences in age groups and genders were observed between the seropositive and seronegative horses using the Chi square X(2) test. Other statistical correlation was also reported concerning horse breed.

Sujets

• Toxoplasma gondii
• Horse
• Antibodies
• Seroprevalence
• MAT
• Tunisia

Informations

• Publié par: biomed
• Publié le: 01 janvier 2011
• Nombre de lectures: 11
• Langue: English

Extrait

Boughattaset al.Parasites & Vectors2011,4:218 http://www.parasitesandvectors.com/content/4/1/218

R E S E A R C H

Seroprevalence ofToxoplasma gondii among horses in Tunisia 1 2 1 1 1* Sonia Boughattas , Ramzi Bergaoui , Rym Essid , Karim Aoun and Aida Bouratbine

Open Access

infection

Abstract Background:The present study was conducted to investigate the serological survey ofToxoplasmaantibodies in local.horses from three major regions: a neighbourhood of a city in the North (Sidi Thabet), a neighbourhood of a city on the coast (Monastir) and a neighbourhood of a city in the middle (Battan) of Tunisia (North of Africa). Methods:A total of 158 serum samples were obtained from clinically healthy horses which consisted of 111 (32 female, 79 male) 210 years old and 47 (11 female, 36 male) older than 10 years. All of the horses were tested for antibodies toT. gondiiusing the Modified Agglutination Test (MAT). Results:According to MAT results, antibodies toT. gondiiwere found in 28 (17.7%) of 158 sera with the titers of 1:20 in 20 horses, 1:40 in 1 horse, 1:80 in 2 horses, 1:160 in 2 horses, 1:320 in 1 horse and≥1:640 in 2 horses. Anti T. gondiiantibodies were found in 18 (16.2%) of 111 horses (210 years old) and 10 (21.2%) of 47 horses (older than 10 years old). Six (13.9%) out of 43 female had antitoxoplasma antibodies and 22 (19.1%) from 115 males remained positive. Conclusion:Statistically significant differences in age groups and genders were observed between the seropositive and seronegative horses using the Chi square X(2) test. Other statistical correlation was also reported concerning horse breed. Keywords:Toxoplasma, horse, antibodies, seroprevalence, MAT, Tunisia

Background Toxoplasmosis is a globally distributed zoonosis with a clinical impact in the unborn fetus and in the immuno suppressed individual. Consumption of undercooked meat has been well established as a major risk factor for humanToxoplasma gondiiinfection worldwide. All warm blood animals are receptive to the parasite but with different rates between the species [1]. With few exceptions horses are considered one of the less sensi tive species to the pathogenic effect ofToxoplasma gon dii[2], however, when infected the parasite is considered to be associated with encephalomyelitis in horses. Moreover, traditionally in Tunisia, the under cooked meat of horse is recommended for pregnant women leading thus to infection of mothers [3]. No data are available on the prevalence of the parasite in

* Correspondence: [e-mail] 1 Laboratoire de Recherche 05SP03, Laboratoire de Parasitologie, Institut Pasteur de Tunis, 13 Place Pasteur BP74, 1002 Tunis Belvédères, Tunisia Full list of author information is available at the end of the article

horses in our country that is why we investigated the determination of its serological survey with different correlations between the geographic location, the gen der, the age and the breed of the animal.

Materials and methods Animal data Blood samples were obtained from 158 horses from dif ferent farms in 3 districts: in the north (Sidi Thabet City), the coast (Monastir City) and the middle (Battan City) of Tunisia (North of Africa). Horses were fed in house with no free grazing. Different breeds of horse were tested: Arabian horse (n = 61), Thoroughbred (n = 3), Barb (n = 31), Arab Pur Sang (n = 24), Breton (n = 2), Pony (n = 9), Arab/Barb (n = 24), Mogod (n = 2), Barb/Breton (n = 2). The different ages of animals were pooled into two groups: one group with an age below 10 years old (n = 111) and the second group with horses older than 10 years old (n = 47). 43 females and 115 males were screened.

© 2011 Boughattas et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.