Temporal variation in the recovery from impairment in adriamycin-induced wound healing in rats

Alagol Haluk, Dinc Soykan, Basgut Bilgen, Abacioglu, Abacioglu Nurettin

Description

An adriamycin-induced impairment of wound healing has been demonstrated experimentally in rats. The purpose of this study is to investigate a possible temporal variation in recovery from the impairment of wound healing caused by adriamycin administration. Methods The subjects were 120 female Spraque-Dawley rats. They were divided into eight groups, undergoing adriamycin administration (8 mg/kg, i.v.) at 9 a.m. or 9 p.m. on day 0 and laparotomy on day 0, 7, 14 or 21. Blast pressures were recorded after the incision line had been opened, and tissue samples were kept at -30°C for later measurement of hydroxyproline levels. Results Adriamycin treatment in rats at 9 p.m. resulted in significantly lower blast pressure levels than treatment at 9 a.m. between days 7 and 21, indicating a lag effect of healing time in wounded tissues. However the decreased hydroxyproline levels were not changed at these days and sessions. Conclusion It is concluded that adriamycin-induced impairment of wound healing in adult female rats exhibits nycthemeral variation.

Informations

• Publié par: biomed
• Publié le: 01 janvier 2007
• Nombre de lectures: 2
• Langue: English

Extrait

Journal of Circadian Rhythms

BioMedCentral

Open Access Research Temporal variation in the recovery from impairment in adriamycin-induced wound healing in rats 1 12 2 Haluk Alagol, Soykan Dinc, Bilgen Basgutand Nurettin Abacioglu*

1 2 Address: Departmentof General Surgery, Ankara Oncology Training and Research Hospital, Demetevler, Ankara, Turkey andDepartment of Pharmacology, Gazi University, 06330, Hipodrom, Ankara, Turkey Email: Haluk Alagol  [e-mail]; Soykan Dinc  [e-mail]; Bilgen Basgut  [e-mail]; Nurettin Abacioglu*  [e-mail] * Corresponding author

Published: 10 October 2007Received: 30 May 2007 Accepted: 10 October 2007 Journal of Circadian Rhythms2007,5:6 doi:10.1186/1740-3391-5-6 This article is available from: http://www.jcircadianrhythms.com/content/5/1/6 © 2007 Alagol et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:An adriamycin-induced impairment of wound healing has been demonstrated experimentally in rats. The purpose of this study is to investigate a possible temporal variation in recovery from the impairment of wound healing caused by adriamycin administration. Methods:The subjects were 120 female Spraque-Dawley rats. They were divided into eight groups, undergoing adriamycin administration (8 mg/kg, i.v.) at 9 a.m. or 9 p.m. on day 0 and laparotomy on day 0, 7, 14 or 21. Blast pressures were recorded after the incision line had been opened, and tissue samples were kept at -30°C for later measurement of hydroxyproline levels. Results:Adriamycin treatment in rats at 9 p.m. resulted in significantly lower blast pressure levels than treatment at 9 a.m. between days 7 and 21, indicating a lag effect of healing time in wounded tissues. However the decreased hydroxyproline levels were not changed at these days and sessions. Conclusion:It is concluded that adriamycin-induced impairment of wound healing in adult female rats exhibits nycthemeral variation.

Background Surgical operation and chemotherapy are concurrent applications in the treatment of various cancer cases. One of the disadvantages of such a concomitant treatment is retardation of healing time in the wounded tissues. The lag effect of healing time of injured tissues is due to, and interrelated with, the circadian dosing time, as dosing time influences the extent of toxicity of some 30 antican

cer drugs, including cytokines and cytostatics, in mice or rats. Selection of the proper circadian dosing time maxi mizes efficacy and minimizes toxicity [13].

Adriamycin is a broadspectrum anthracyclinederivative intercalating agent with many clinical side effects. Adri amycininduced impairment of wound healing was first demonstrated by Devereaux et al. in rats [4,5], and many

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