Carbonic anhydrase II [Elektronische Ressource] : a model system for artificial copper center design, protein-guided cycloadditions, tethering screenings and fragment-based lead discovery / vorgelegt von Johannes Schulze Wischeler

Jsw

Sujets

• Naturwissenschaften

Informations

• Publié par: philipps-universitat_marburg
• Publié le: 01 janvier 2010
• Nombre de lectures: 24
• Poids de l'ouvrage: 4 Mo

Extrait

Carbonic Anhydrase II: A Model System for Artificial
Copper Center Design, Protein-guided Cycloadditions,
Tethering Screenings and Fragment-based Lead Discovery


Dissertation
zur
Erlangung des Doktorgrades
der Naturwissenschaften
(Dr. rer. nat.)



dem Fachbereich Pharmazie
der PHILIPPS-UNIVERSITÄT MARBURG
vorgelegt von
Johannes Schulze Wischeler
aus Bergisch-Gladbach


Marburg/Lahn 2010

















Vom Fachbereich Pharmazie der Philipps-Universität Marburg
als Dissertation angenommen am: 24.06.2010

Erstgutachter: Prof. Dr. Gerhard Klebe
Zweitgutachter: Prof. Dr. Ulrich Koert
Tag der mündlichen Prüfung: 25.06.2010
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Die Untersuchungen zur vorliegenden Arbeit wurden auf Anregung von Herrn Prof. Dr.
Gerhard Klebe am Institut für Pharmazeutische Chemie des Fachbereichs Pharmazie der
Philipps-Universität Marburg in der Zeit von Januar 2007 bis Juni 2010 durchgeführt.


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Abbreviations
Abbreviations
°C Celsius
ζ (crystallography) Sigma-level of an electron density map (refers to probability of
finding an electron within the volume enclosed by the map. It
provides a way of accessing the "noise" level of the respective
electron density map.)
-10Å Ångström (1Å = 10 m)
A Absorption at 600 nm 600
Ala Alanine
Arg Arginine
Amp Ampicillin
Asn Asparagine
Asp Aspartic acid
B-factor Debye-Waller-factor
CA Carbonic anhydrase
Cam Chloramphenicole
CSD Cambridge Structural Database
Cys Cysteine
DMSO Dimethylsulfoxide
dNTP Desoxynucleosidtriphosphat
DTNB 5,5'-dithiobis-(2-nitrobenzoic acid)
DTT Dithiothreitol
E. coli Escherichia coli
F calculated structure amplitudes c
F observed structure amplitudes o
Gln Glutamine
Glu Glutamic acid
Gly Glycine
GST Glutathione-S-Transferase
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Abbreviations
h Hour
H-bond Hydrogen bond
His Histidine
HPLC-MS High pressure liquid chromatography-mass spectrometry
Ile Isoleucine
IPTG Isopropylthio-β-galactosid
I.U. International unit
K Kelvin
k Kilo
Da Dalton
K Competitive inhibition constant i
K Michaelis Menten constant m
Leu Leucine
Lys Lysine
M Molarity (mol/l)
Met Methionine
Min Minute
NMR Nuclear magnetic resonance
NTP Nucleosidtriphosphat
PAGE Polyacrylamid gel electrophoresis
PBS Phosphate buffered saline
PCR Polymerase chain reaction
PDB Protein data bank
pH Potentialis hydrogenii
Phe Phenylalanine
Pro Proline
R-factor Reliability factor
RMSD Root mean square deviation
rpm Revolutions per minute
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Abbreviations
SDS Sodiumdodecylsulfate
Ser Serine
Thr Threonine
Tris Tris(hydroxymethyl)-aminomethane
Trp Tryptophane
Tyr Tyrosine
Val Valine
w/v Weight per volume
WT wild type
X-ray Electromagnetic radiation, also called Röntgen radiation
(wavelength: 10 – 0.01 nanometers, typical wavelengths used for
X-ray crystallography are around 1 Å (= 0.1 nanometer), which is
in the range of covalent chemical bonds.)
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