Water permeability of plant cells measured by pressure probes [Elektronische Ressource] : effects of light and turgor, and the role of unstirred layers / von Yangmin Kim

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Water permeability of plant cells measured by pressure probes: effects of light and turgor, and the role of unstirred layers Inaugural-Dissertation zur Erlangung des Doktorgrades der Fakultät Biologie, Chemie und Geowissenschaften der Universität Bayreuth von Yangmin Kim aus Incheon, Süd-Korea Bayreuth, im Juli 2008 II Die vorliegende Arbeit wurde am Lehrstuhl für Pflanzenökologie der Universität Bayreuth unter der Leitung von Prof. Dr. E. Steudle durchgeführt. Sie entstand im Zeitraum von Oktober 2004 bis Juli 2008, gefördert durch ein Stipendium des Deutschen Akademischen Austauschdienstes (DAAD). 1. Gutachter: Prof. Dr. E. Steudle 2. Gutachter: Prof. Dr. S. Clemens Tag der Einreichung: 16.07.2008 Tag der mündlichen Prüfung: 01.12.2008 Prüfungsausschuss: Prof. Dr. E. Komor (Vorsitz) Prof. Dr. E. Steudle Prof. Dr. S. Clemens Prof. Dr. T. Fischer Prof. Dr. G. Gebauer Vollständiger Abdruck der von der Fakultät für Biologie, Chemie und Geowissenschaften der Universität Bayreuth genehmigten Dissertation zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) III To see… a heaven in a wild flower, Hold… eternity in an hour. William Blake Sun light brings a plant not only energy but also thirst, Unless the plant knows how to take care of water.
Publié le : mardi 1 janvier 2008
Lecture(s) : 41
Tags :
Source : OPUS.UB.UNI-BAYREUTH.DE/VOLLTEXTE/2008/489/PDF/KIMDISS.PDF
Nombre de pages : 215
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Water permeability of plant cells measured by
pressure probes: effects of light and turgor,
and the role of unstirred layers








Inaugural-Dissertation
zur Erlangung des Doktorgrades
der Fakultät Biologie, Chemie und Geowissenschaften
der Universität Bayreuth




von
Yangmin Kim
aus Incheon, Süd-Korea



Bayreuth, im Juli 2008


II


Die vorliegende Arbeit wurde am Lehrstuhl für Pflanzenökologie der Universität
Bayreuth unter der Leitung von Prof. Dr. E. Steudle durchgeführt. Sie entstand im
Zeitraum von Oktober 2004 bis Juli 2008, gefördert durch ein Stipendium des
Deutschen Akademischen Austauschdienstes (DAAD).

1. Gutachter: Prof. Dr. E. Steudle
2. Gutachter: Prof. Dr. S. Clemens
Tag der Einreichung: 16.07.2008
Tag der mündlichen Prüfung: 01.12.2008

Prüfungsausschuss:

Prof. Dr. E. Komor (Vorsitz)
Prof. Dr. E. Steudle
Prof. Dr. S. Clemens
Prof. Dr. T. Fischer
Prof. Dr. G. Gebauer








Vollständiger Abdruck der von der Fakultät für Biologie, Chemie und
Geowissenschaften der Universität Bayreuth genehmigten Dissertation zur Erlangung
des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.)

III











To see… a heaven in a wild flower,
Hold… eternity in an hour.

William Blake












Sun light brings a plant not only energy but also thirst,
Unless the plant knows how to take care of water.



IV

This dissertation is submitted as a “Cumulative Thesis” that covers four (4)
publications; three (3) printed articles and one (1) article that is in press in Journal of
Experimental Botany. In order to clarify the publications, they are listed below.

Printed articles:
1. Ye Q., Kim Y., Steudle E. 2006 A re-examination of the minor role of
unstirred layers during the measurement of transport coefficients of Chara
corallina internodes with the cell pressure probe. Plant, Cell and
Environment 29, 964-980 (Chapter 2).

2. Kim Y., Ye Q., Reinhardt H., Steudle E. 2006 Further quantification of the
role of internal unstirred layers during the measurement of transport coefficients
in giant internodes of Chara by a new stop-flow technique. Journal of
Experimental Botany 57, 4133-4144 (Chapter 3).

3. Kim Y.X., Steudle E. 2007 Light and turgor affect the water permeability
(aquaporins) of parenchyma cells in the midrib of leaves of Zea mays. Journal
of Experimental Botany 58, 4119-4129 (Chapter 4).

4. Kim Y.X., Steudle E. 2008 Gating of aquaporins by light and reactive oxygen
species in leaf parenchyma cells of the midrib of Zea mays. Journal of
Experimental Botany (in press) (Chapter 5).
V

Declaration of the self-contribution of research articles

The thesis is compiled with four research articles, which include different research
work. Most of the research work in the thesis was carried out by myself independently
at the Department of Plant Ecology, University of Bayreuth under guidance of Prof.
Steudle.

In Chapter 2, I participated in experiments, and contributed to most of the theory parts
applying analytical solutions. I did substantially contribute to the discussion and to the
writing of the manuscript.

In Chapter 3, I participated in experiments, developed the computer
analysis/simulations, and wrote most part of manuscripts. Other contributors were Dr.
Qing Ye, a former Ph. D. student and Mr. Hagen Reinhardt, a Diplom student.

In Chapters 4 and 5, all the experiments were done by myself in addition to the writing
of the manuscripts.

All published articles can be downloaded from the world wide web:
http://www.uni-bayreuth.de/departments/planta/research/steudle/index.html. VI

Acknowledgements

First of all, I would like to thank Prof. Dr. E. Steudle, Department of Plant Ecology,
Bayreuth University, for his guidance during my Ph. D. His passion on the research
that I felt at the moment I met him during his teaching in Korea in 2003 has influenced
me during the whole period of my study. He showed me what a scientist could be. I
thank him not only for introducing and teaching me the subject, plant water relations,
but also for letting me realize the pleasure to study. Long and harsh discussions (which
some people would think even derogatory) were what I have learned and will try to do
through my career.

I thank Burkhard Stumpf, Department of Plant Ecology, Bayreuth University, for his
expert technical supports and great help in many aspects. He is a magic person who
can solve any technical problems that can happen in the lab. I appreciate all the cares
he gave me for the pleasant stay in Bayreuth.

I am grateful to Prof. Carol A Peterson, Department of Biology, University of
Waterloo, Canada, for her teaching of plant anatomy during various stays in the lab.
Being brought as an engineer, this was quite new for me in which I had been totally
ignorant before I met her. Her enthusiasm and knowledge on plant anatomy impressed
me. It would be solely because of her that I became less ignorant of the plant anatomy.

I thank all the colleagues from various countries with whom I worked in Bayreuth. I
learned many things from you: Seonghee Lee, Kosala Ranathunge, Qing Ye,
Lukasz Kotula, Ewa Przedpelska, Prof. Suiqi Zhang, Binbin Liu, Chris Meyer,
Thorsten Knipfer, Hagen Reinhardt, Ola, Changxing, Lying, Debasish Das, and
Ankur Joshi.

Special thanks to Jakob for his corrections on German summary, to Kosala for
English corrections, to Moonyoung for giving me an inspiration of the prologue, and
to Taeyoung for uncountable endurance during my study. VII



I would like to thank all those to whom I am indebted: Prof. SJ Lee, Department of
Mechanical Engineering, POSTECH, Korea, for making me start a research, Prof. CO
Lee, Department of Life Science, POSTECH, Korea, and Biochemistry, Structural
Biology and Chemistry, The Rockefeller University, USA, for being my role model so
that I could stay in science, Prof. Jack Dainty, Department of Botany, University of
Toronto, Canada, for allowing me to meet the legend in plant biophysics, Gesine
Steudle, Nano-optics, Humboldt Universität for encouraging me to come to Germany,
Reiner Krug and Heike in the greenhouse for taking care of my plants, and those
whom I met during many pressure probe courses for giving me many inspirations.

I thank all of my friends who shared a certain time in Bayreuth: Bora, Buyoung,
Eunjung, Eunsook, Eunyoung, Family Ha, Family Handel, Family Jung, Family
Mader, Family Otto, Family Park Hoseon, Family Park Youngki, Hortence,
Insuk, Jashri, Joe, Junghee, Kathie, Prof. Kang, Prof. Kleiner, Punnagai, Seoleun,
Sooim, Suna, Susanne, Yongsuk, and Yoolim.

Deutscher Akademischer Austauschdienst, DAAD is acknowledged for the grant.
Thanks are given to my parents, Namsuk Kim and Myeongja Kim, and my brother
Kyungrae for their trust and support. VIII
IX
Contents

I Detailed Summary......................................................................................1

1 General introduction.........................................................................................3
1.1 Water and solute flows..................................................................................6
1.1.1 Elastic modulus.............................................................................................9
1.1.2 Hydrostatic pressure relaxations.................................................................10
1.1.3 Osmotic pressure relaxations in the presence of a permeating solute.........11
1.2 Role of living cells in overall leaf hydraulic conductance (K ).............14 leaf
1.2.1 Water transport pathways in leaves.............................................................14
1.2.2 K regulation by light................................................................................14 leaf
1.2.3 Change in cell water permeability (cell Lp) and gating of
aquaporins in response to light...................................................................16
1.2.4 Implication of K regulation by light in whole plants..............................17 leaf
1.3 Unstirred layers (USLs)..............................................................................17
1.3.1 Sweep away effect......................................................................................19
1.3.2 Gradient dissipation effect..........................................................................20
1.3.3 Analytical solution assuming the stagnant internal USL............................22
1.3.4 Stop flow technique....................................................................................24
1.3.4.1 Simulation of diffusion inside the cell.........................................25
1.3.4.2 Simulation of effects of internal USLs during stop flow.............26
1.4 Aims of the research....................................................................................28
1.5 Materials and methods............................................................31
1.5.1 Growth of corn plants.................................................................................31
1.5.2 Growth of Chara corallina.........................................................................31
1.5.3 Pressure probe for Chara internodes..........................................................31
1.5.4 or higher plant cells..........................................................31
1.5.5 Perfusion technique....................................................................................34
1.6 Results and discussions..................................................................................35
1.6.1 Quantification of the role of unstirred layers during measurements of
transport parameters of water and solutes................................................. 35 X
1.6.2 Quantification of the role of internal unstirred layers during
the measurement of solute permeability coefficient (P )...........................36 s
1.6.3 Effects of low light and turgor on water permeability of leaf cells............37
1.6.4 Effects of high light and OH* on water permeability of leaf cells.............38
1.7 General conclusion........................................................................................40
1.8 Short summary..............................................................................................42
1.9 References......................................................................................................44

II Publications..............................................................................55

2 A re-examination of the minor role of unstirred layers
during the measurement of transport coefficients of Chara corallina
internodes with the cell pressure probe......................................................57

3 Further quantification of the role of internal unstirred layers
during the measurement of transport coefficients
in giant internodes of Chara by a new stop-flow technique.....................101

4 Light and turgor affect the water permeability (aquaporins) of
parenchyma cells in the midrib of leaves of Zea mays.............................133

5 Gating of aquaporins by light and reactive oxygen species
in leaf parenchyma cells of the midrib of Zea mays.................................165

6 Summary.......................................................................................................193
7 Zusammenfassung........................................................................................199
8 Erklärung......................................................................................................205




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