Niveau: Supérieur, Doctorat, Bac+8
lato gH gen Co Mic , 2 ru S 44, VH promoter-GFP reporter gene linked to the 3VLCR region and the EA ta 164 A complex interplay of multiple regulatory elements is responsible for tissue-specific and stage-specific regulation of both transcription and rearrangements of the IgH chain locus. Several events such as the germline transcription of the CA region and the initiation of VDJ rearrangements are regulated by upstream elements including the VH promoter and the EA intronic enhancer [1]. In addition, four lym- phoid-specific transcriptional enhancers (namely hs3a, hs1,2, hs3b and hs4) have been located in a 3V regulatory region downstream the locus. It has been suggested that the latter altogether act as a locus control region (LCR). The [3] reported that transgenic mice bearing a h-globin report- er gene linked to the 3VLCR displayed a high level, position-independent and B cell-specific expression. Our knowledge of the role of these 3Venhancers mostly derives from in vitro studies [4]. Thus, transient transfection experi- ments and reporter gene assays revealed that the four 3V enhancers are rather weak by themselves but that their combination display a strong transcriptional synergy espe- cially when the normal palindromic arrangement is respected [4–6]. All these studies provided important information about synergies between enhancers and sug- gested a B cell-restricted activity of the 3V IgH elements.
- fragment
- genomic dna
- elements thus appears
- vlcr transgenic
- ea- gfp
- yielded both
- gene
- kb pcr
- gfp