5.5 Å Structure of the Eukaryotic Ribosome [Elektronische Ressource] / Jean-Paul Armache. Betreuer: Roland Beckmann

Jean-Paul Armache

Sujets

• Biologie

Informations

• Publié par: ludwig-maximilians-universitat_munchen
• Publié le: 01 janvier 2011
• Nombre de lectures: 55
• Langue: Deutsch
• Poids de l'ouvrage: 26 Mo

Extrait

Dissertation zur Erlangung des Doktorgrades der Fakultät fur Chemie und
Pharmazie der Ludwig-Maximilians-Universität München

5.5 Å Structure of the Eukaryotic
Ribosome





Jean-Paul Armache
aus Łódź, Polen

2011Erklärung
Diese Dissertation wurde im Sinne von § 13 Abs. 3 bzw. 4 der Promotionsordnung vom 29.
Januar 1998 von Herrn Prof. Dr. Roland Beckmann betreut.


Ehrenwörtliche Versicherung
Diese Dissertation wurde selbstständig, ohne unerlaubte Hilfe erarbeitet.

München, am 25.02.2011
Jean-Paul Armache









Dissertation eingereicht am: 25.02.2011
1. Gutachter: Herr Prof. Dr. Roland Beckmann
2. Gutachter: Herr Prof. Dr. Karl-Peter Hopfner
Mündliche Prüfung am: 04.05.2011 Table of Contents

Acgedwtemelosknn ................................................................................................................6
Summary ................................................................................................................................8
1  I........................9
1.1  Th  F    G  Ia ...................................................................................9
1.2  R s ..................................................10
1.3  Ea  R .................................................................................................11
1.4  R  B...11
1.5  Traa  Ma ...............................................................................................12
1.5  P -­‐bod  n Fmaro tnoi.............................14
1.6  Ra   .....................................................................................................15
1.7  S    Ea  R   -­‐  I ......................................................16
1.8  A  (O      T) ..........................................................16
2  Maa  a  M.....................................................................18
2.1  Sa  Paa .....................................18
2.1.1      Sa  Paa  (  Ha  e  a.,  2004) ............................18
2.1.2  Sac  mchesyaor aeisicevr  Knockout  Strains  Sample  Preparatio..........................n 19
2.1.3  Aaa  R  Sa  Paa  (  M    a.,  2010) ..................20
2.2  C -­‐Elotec  rn Mypcsooicr ...........................................................................................21
2.2.1  Sa  Paa..............................22
2.2.2  Ia  Ga .................................................................................................22
2.2.3  Ia  Prescgonsi..23
2.2.4  R  Da .....................................................................................25
2.2.5  Ma  D..............................25
2.3  M .....................................................................................................................25
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2.3.1  P  Tea  S ................................................................................26
2.3.2  Caa/H  M........26
2.3.3  Sa  S  P ............................................................................27
2.3.4  D -­‐bad  M  h  Snda  S  P ..............................28
2.3.5  R  B  F ..................................................................................................29
2.3.6  Ma  Da  F  F......29
2.3.6  M  W  Sa ..............................................................................29
2.4  F ........................................................31
3  R...............................................................................................32
3.1  A  C -­‐EM  Ma    a  Ea  R  a  5.5  ......................32
3.1.1  D .................................................................................................................33
3.1.2  Fa   ................................................................................................33
3.2  M  o    D...........................39
3.2.1  C  C ....................................................................................................39
3.2.2  Ua  Eaa -­‐Spciecif  Psnteoir...42
3.2.3  Ea -­‐Specf  cii P  rnteoi Esxntoseni ..................................................................47
3.3  Protein  Localization .....................................53
3.3.1  Sa  S  P..........................................................54
3.3.1.3  Sa  S  B ................................................................58
3.3.2  Large  Subunit  Proteins..........................................................58
3.3.3  Th  R maiednr .....................................................................68
4  C  a  D...............................................................70
4.1  C -­‐Elotec  rn Mypcs  ooicr Map  fo    ..................70
4.1.1  Fa  V  a  G  R ....................................................................70
4.1.2  I  O  E  R    Ea  R ................70
4.1.3  C -­‐EM  a        aa  a ...........................................71

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4.2  M .........................................................................................................................72
4.2.1  Loc alzati o  in fo  et  h Nv  oel Pteoisrn .........................................................................72
4.2.2  R    P ...................................74
4.2.3  I  F  a  Ia .........................................................................83
4.2.4  P      .....................85
4.2.5  Ra      M .........................................................................................88
4.2.6  Caa  Aa    Ca  S.......................90
4.3  R      L    E ..............................................................................94
5  Sa ...........................................................98
6  A.........................................................................................100
7  C  a ..............................................127
8  R.......................................................................................129


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odleAcknowledgements

I would like to thank my supervisor, Prof. Roland Beckmann for allowing me to conduct
research in his group in the Gene Center. I am grateful for his trust in a computer scientist
working in Structural Biology, for the unique working conditions, atmosphere and his
constant support.
Many thanks to Dr. Daniel Wilson for his patience, availability, openness to discussions and
the immeasurable amount of time and work he invested in this project.
I wish to express a deep sense of gratitude to a number of people I had pleasure of working
with. Dr. Thomas Becker, who taught me everything I know about cryo-electron microscopy
and always had time to help me, no matter how busy he was; Dr. Shashi Bhushan, for the
fruitful collaboration and the tremendous amount of high-quality data no one else could have
acquired; Andreas Anger, Alexander Jarasch and Elizabeth Villa for all the time spent
together, the time