A biomimetic model membrane system on oxidic surfaces designed for the investigation of cytochrome c-oxidase and other membrane proteins by fluorescence and waveguide spectroscopy [Elektronische Ressource] / vorgelegt von Asmorom Afewerki Kibrom

Description

UnivAFBiomimeticJuliMounddelAfewMemderbraneSystemderonorgelegtOxidicorenSurfacesGradesDesignedhaftenforhtheChemie,InwissenscvbestigationMainzofonCytoKibromAsmara/EritreaomedesDoktorOxidaseandimotheracMembbranehProteinsPharmaziebGeoyhaftenJohannes-GutenandergWersit?tavvveguideAsmoromSperkigebyinDissertationMainz,zur2010Erlangungterstatter:Dekiian:mProf.Dr.agD.Sc.hen2.WholfgangTHofmeisterder1.?ndlicPr?fung:hterstatter:onDiehnologyvInstituteorliegendeinArbAugusteitTwurdeinamZeit2006k-Institutangefertigt.f?rofPecolymer-(AIT)WienhderungvinMaiMainzbisund2009amiiiAustriandospThewtransmemortedbraneITO,pimpotenometialadierencehivhptBLM.ptBLMavideistheinassoyotentowtheashelatingactivityofdialysisgoSurfaceomeanisotropmetryoxidaseo-braneCcOwismeasurementhetheterminalbenzymeu-(complexIV)erinfortheelectronA)transpacortbtationhainsub-offormedtheElectromitoysealinghondria.proThewenzymeWS)systemtheer.ofofCcOOthe2eto2anHw2O,tetheredtherebynelectronsareoltagetransferred80fromorvaomelaastosoftCcO.ptBLMElectronvidingtransferelwithinacidthetoCcOfromiswtoinprotondenedtransloatoConsequenI.

Sujets

• Naturwissenschaften

Informations

• Publié par: johannes_gutenberg-universitat_mainz
• Publié le: 01 janvier 2010
• Nombre de lectures: 11
• Langue: English
• Poids de l'ouvrage: 5 Mo

Extrait

Univ
A
F
Biomimetic
Juli
Mo
und
del
Afew
Mem
der
brane

System
der
on
orgelegt
Oxidic
oren
Surfaces
Grades
Designed
haften
for
h
the
Chemie,
In
wissensc
v
b
estigation
Mainz
of
on
Cyto
Kibrom

Asmara/Eritrea
ome
des

Doktor
Oxidase

and
im
other
ac
Mem
b
brane
h
Proteins
Pharmazie
b
Geo
y
haften

Johannes-Guten
and
erg
W
ersit?t
a
v
v
v
eguide
Asmorom
Sp
erki

geb
y
in
Dissertation
Mainz,
zur
2010
Erlangungterstatter:
Dek
ii
an:
m
Prof.

Dr.
ag
D.Sc.
hen

2.
W
h
olfgang
T
Hofmeister
der
1.
?ndlic

Pr?fung:
h
terstatter:on
Die
hnology
v
Institute
orliegende
in
Arb
August
eit
T
wurde
in
am
Zeit

2006
k-Institut
angefertigt.
f?r
of
P
ec
olymer-
(AIT)

Wien
h
der
ung
v
in
Mai
Mainz
bis
und
2009
am
iii
AustrianΔφm
Δφm
Δφm
Δφm
Δφm
≈ ⋅
≈
Δφm
w



b
ome
y

o
oxidase


form.
CcO
in
is
k
the
via
terminal
sho
enzyme

(complex
w
IV)
external
in
in
the
These
electron
the
transp
groups,
ort
ound

unit
hain

of
prop
the
a
mito
after


hondria.
gel-supp
The
in
enzyme
as

BLM
the
(0.05/100

w
of
in
O
a
2
pro
to

2
CcO
H
ans
2
ell
O,
hed
thereb
w
y
using
electrons

are
o
transferred
1
from
plasmon



y
ome
lipid

in
to

CcO.
rated
Electron
a
transfer
p
within
ptBLM
the
a
CcO

is
ts


to

proton
tial
translo
y

wn
Consequen
a
tly
the
,
used
a
'cushion'
dierence
on
in
the
proton


(NT
tration
whic
o
Par
v

er
as
the
a
inner
orien
mito
his-tag

its
hondrial
The
mem
then
brane
in-situ
and
eads.
a
ical
mem

brane
ed
p
electrical
oten
(
tial
W
dierence
).
y

activit
eguide

(SPR/O
are

generated.
the
The
of
transmem
y
brane
oltam-
p

oten

tial
that
the

to
to

ptBLM

activ
b
mem
e
tial
measured
gel-supp
b
y
y
oltage

eform
sp
b

sp
y
Reference
using
a
a
vided
p
ersion
oten
of
tial
R
sensitiv
P
e
hanges
dy
ptBLM
e.
v
Before
measured
quan
The
titativ
pro
e
for
results
estigations


b
as
e
as

soft
from
for

ptBLM
h
ITO,
in
viding
v

estigations,
el

helating
measuremen
acid
ts
A)
using
to
dened
h
electrical
from
p
ac
oten

tials

applied
w
to
b
the
in
lipid
w
mem
dened
brane
tation
ha
a
v

e
to
to
sub-
b
I.
e
ptBLM

as
Here,
formed

y
measuremen
dialysis
t
bio-b
of
Electro
asso
hem-

imp
is
sp
in
y
a
w
mo
go
del
d
mem
sealing
brane
erties
with
brane
CcO
M
are
transmem
pre-
2
sen
Surface
ted.
resonance
dierence
w
tial
v
oten
sp
is
y

WS)
b
an
y
anisotrop
dened
of
external
system
p
formation
oten
the
tials
bila
applied
er.
to
v
the
metry
lipid
the
mem
of
brane.

Therefore,
ome
a
demonstrated
mo
CcO
del
as
mem
o-
brane
in
system
the

orted
the
in
protein-tethered
functionally
bila
e
y
The
er
brane
lipid
oten
mem-

brane
a
(ptBLM)
orted
w
b
as
an
dev
v
elop
w
ed
v
on
w
the
measured
transparen
y
t,



substrate
.
indium
measuremen
tin-o
using
xide
tethered
(ITO).
pro
ITO
a
enables
v
sim

ultaneous
terms
implemen
the
tation
parameter
of
n
electro
mV).

oten


and

u-
the

b
or
external

oltage
w
ere
a
do
p
to
of
80
mV.
CcO
data
a
vide
iv
basis
thin
future
h
v
ydrogel
of
la
self-generated
y
er
v
of
eguide
in

ptBLM.
hniques.
A.
Contents
.
1
.
Intro
.

.
1
.
1.1
.
Mem
.
brane
.
Mo
.
del
.
Systems
(EIS)
for
.
the
.
In
.
v
rent
estigation
bly
of
CcO
Mem
of
brane
.
Proteins
.
.
.
2
Electro
1.1.1
.
T
.
ethered
.
BLM
.
on
.
metal
eguide
electro
.
des
Memb
.
W
.
.
.
.
.
of
.
.
.
.
.
y
.
T
.
.
.
.
.
.
.
.
.
.
.
.
.

.
.
.
2.1.3
.
.
3
.
1.1.2
.
Protein-tethered
2.2
BLM
.
on
.
metal
.
electro
.
des

.
.
.
.
.
.
.
3
.
System
.
Protein-tethered
.
.
.
.
.
.
.
monola
.
.
.
48
.
surface
5
ellipsometry
1.2
.

.
of
54
the
Si
Mem
.
brane
.
P
ITO
oten
.
tial
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
26
.

.

.
.
.
.
.
.
.
v
.
.
.
.
.
.
7
.
1.2.1
.
P
.
assiv
.
e
.
ion
.
transp
.
ort
.
.
.
.
.
.
.
.
.
.
.
.
36
.
a
.

.
.
.
.
.
.
.
.
.
.
.
.
.
of
.
Mo
.
T
.
47
.
on
.
.
.
.
.
.
.
.
.
.
.
3.1.1
.
the
8
er
1.2.2
.
The
.
respiratory
.

F
hain
silanized
.
binding
.
b
.
.
.
.
.
.
.
.
.
.
.
.
.
F
.
ptBLM
.
afer
.
.
.
.
.
.
.
56
.
BLMs
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
59
.
.
.
.
12
.
1.2.3
.
Cyto
.

.
hrome
.

.
o
.
xidase
.
and
.
activ
.
e
.
proton
.
transp
.
ort
.
.
2.1.2
.

.
Imp
.
Sp
.
y
.
.
.
.
.
.
16
.
1.2.4
.
The
26
mem

brane
oltammetry
p
.
oten
.
tial
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
34
.
Ellipsometry
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.
20
.
1.3
.
Aim
.
of
.
this
.
W
.
ork
2.3
.
W
.
v
.
Sp
.
y
.
.
.
.
.
.
.
.
.
.
.
.
.
.
.