Chup1 – a chloroplast movement protein and its interactions Dissertation an der Fakultät für Biologie der Ludwig-Maximilians-Universität München vorgelegt von Serena Schmidt von Braun München, 2008 II Erstgutachter: Prof. Dr. Enrico Schleiff Zweitgutachter: Prof. Dr. Manfred Schliwa Eingereicht am: 08.05.2008 Tag der mündlichen Prüfung: 11.07.2008 III Figure 1 Excerpt from: Böhm JA (1856) Beiträge zur näheren Kenntnis des Chlorophylls. S.B. Akad. Wiss. Wien, Math.-nat. Kl. 22: 479-498 IV CONTENTS CONTENTS 1.1 ABSTRACT VII 1.2 ZUSAMMENFASSUNG VIII 2 INTRODUCTION 1 2.1 CHLOROPLAST MOVEMENT 1 2.2 THE SIGNAL3 2.2.1 The Light Receptors3 2.2.2 The modulator calcium5 2.3 THE TRACKS6 2.4 THE MOTOR FOR MOVEMENT?8 2.5 COMPONENTS-INVOLVED IN CHLOROPLAST MOVEMENT 10 2.5.1 Chup1 10 2.5.2 Jac1 11 2.5.3 The Pmi family 11 2.6 AIM 13 3 MATERIALS AND METHODS 14 3.1 MATERIALS 14 3.1.1 Chemicals 14 3.1.2 Enzymes and Kits 3.1.3 E.coli strains 14 3.1.4 Plant material 15 3.1.5 Oligonucleotides 15 3.1.6 Vectors 15 3.1.7 Antibodies 16 3.1.8 Other material 16 3.1.9 Services 16 3.1.10 Further Instruments and Equipment 16 3.1.11 Bioinformatic tools 3.2 METHODS 18 3.2.1 General Methods 18 3.2.2 Molecular Methods 3.2.3 Cellular Methods 19 3.2.4 Biochemical Methods 20 3.2.