Heterosis in the freezing tolerance of Arabidopsis thaliana (L.) Heynh [Elektronische Ressource] / von Marina Korn

Marina Korn

Description

Max- Planck-Institut für Molekulare Pflanzenphysiologie Arbeitsgruppe PD Dr. Dirk K. Hincha „ Heterosis in the Freezing tolerance of Arabidopsis thaliana (L.) Heynh.” D i s s e r t a t i o n zur Erlangung des akademischen Grades doctor rerum horticulturarum (Dr. rer. hort.) eingereicht an der Landwirtschaftlich-Gärtnerischen Fakultät der Humboldt-Universität zu Berlin von Dipl.-Ing. agr. Marina Korn Präsidentin / Präsident der Humboldt-Universität zu Berlin: Prof. Dr. Dr. h.c. Christoph Markschies Dekan der Landwirtschaftlich-Gärtnerischen Fakultät: Prof. Dr. Dr. h.c. Otto Kaufmann Gutachter: 1. Prof. Dr. Frank Pohlheim 2. PD Dr. Dirk Hincha Datum der Einreichung: 07. Juli 2008 Tag der mündlichen Prüfung: 25. November 2008 to my familyII Table of contents I Table of contents II List of tables presented in the general part ...................................................................... V III List of figures presented in the general part................................................................... V IV Abbreviations....................................................................................................................VI 1. Summary............................................................................................................................... 1 2. General introduction ..................

Informations

• Publié par: humboldt-universitat_zu_berlin
• Publié le: 01 janvier 2010
• Nombre de lectures: 23
• Langue: Deutsch
• Poids de l'ouvrage: 3 Mo

Extrait

Max- Planck-Institut für Molekulare Pflanzenphysiologie Arbeitsgruppe PD Dr. Dirk K. Hincha    Heterosis in the Freezing tolerance ofArabidopsis thaliana(L.) Heynh.   

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D i s s e r t a t i o n

zur Erlangung des akademischen Grades doctor rerum horticulturarum (Dr. rer. hort.)

eingereicht an der Landwirtschaftlich-Gärtnerischen Fakultät der Humboldt-Universität zu Berlin

von Dipl.-Ing. agr. Marina Korn 

  Präsidentin / Präsident der Humboldt-Universität zu Berlin: Prof. Dr. Dr. h.c. Christoph Markschies  

Dekan der Landwirtschaftlich-Gärtnerischen Fakultät: Prof. Dr. Dr. h.c. Otto Kaufmann   Gutachter: 1. Prof. Dr. Frank Pohlheim  2. PD Dr. Dirk Hincha 

 Datum der Einreichung: Tag der mündlichen Prüfung:

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07. Juli 2008 25. November 2008

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Table of contents

I Table of contents

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II List of tables presented in the general part ...................................................................... V

III List of figures presented in the general part................................................................... V

IV Abbreviations ....................................................................................................................VI

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1. Summary............................................................................................................................... 1

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2. General introduction ........................................................................................................... 3

2.1.Arabidopsis thaliana (L.)Heynh. ................................................................................... 3

2.2. Heterosis ......................................................................................................................... 4

2.3. Stress in plants ................................................................................................................ 7

2.3.1. Cold stress................................................................................................................ 9

2.3.1.1. The impact of low temperatures/ cold stress on plants ..................................... 9

2.3.1.2. Metabolic effects of cold in plants.................................................................. 13

2.4. Metabolite Profiling ...................................................................................................... 15

2.4.1. Methods for metabolite determination................................................................... 15

2.5. The Aim of this work .................................................................................................... 17

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3. Paper 1 (P1) ........................................................................................................................ 19

3.1. Abstract ......................................................................................................................... 20

3.2. Introduction................................................................................................................... 20

3.3. Materials and methods .................................................................................................. 22

3.4. Results........................................................................................................................... 24

3.5. Discussion ..................................................................................................................... 39

3.6. Acknowledgments......................................................................................................... 44

3.7. References..................................................................................................................... 44

3.8.Supplementarymaterial................................................................................................47

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4. Manuscript 1 (M1) ............................................................................................................. 50

4.1. Abstract ......................................................................................................................... 51

4.2. Results & Discussion .................................................................................................... 51

4.3. Acknowledgments......................................................................................................... 56

4.4. References..................................................................................................................... 56

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III

Table of contents

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4.5. Supplemental Material ..................................................................................................58

5. General discussion..............................................................................................................61

5.1. Heterosis in the freezing tolerance ofA. thalianahybrids ............................................61 5.2. Heterosis in the accumulation of compatible solutes and secondary metabolites.........64

5.2.1. Compatible solutes .................................................................................................64

5.2.2. Flavonoids ..............................................................................................................66

5.3. The role of the TCA- cycle under cold stress and in heterosis in freezing tolerance....67

5.4. Conclusions and Outlook ..............................................................................................68

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6. Literature cited in the general part ..................................................................................70

7. Deutsche Zusammenfassung .............................................................................................75

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V Acknowledgments...............................................................................................................77

VI List of publications............................................................................................................78

VII Conferences......................................................................................................................79

VIII Eidesstattliche Erklärung

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List of tables and figures presented in the general part

II List of tables presented in the general part

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Table 1. Calculation of absolute and relative heterosis ........................................................... 5

Table 2. List of different abiotic and biotic plant stressors...................................................... 7

Table 3: Lethal temperature (LT50) for non acclimated (NA) and ...........................................

acclimated (ACC) ................................................................................................... 61

Table 4: Mid-Parent-Heterosis (M, blue) and Best-Parent-Heterosis (B, orange) in ...............

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freezing tolerance (LT 50 ), sugar and proline content. .............................................

III List of figures presented in the general part

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Figure 1. Kinds of heterosis .................................................................................................... 4

Figure 2: Independent component analysis (ICA) of non acclimated and ...............................

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acclimated accessions . .......................................................................................... 65

Figure 3: Hybridization design. ............................................................................................. 69

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V

APPI

BPH

Can

CDPK

CBF

CAS

ICA

ICE

ACC

IL

IUPAC

KEGG

LC

LEA

Ler

VI

 ABA

IV Abbreviations

Abbreviations

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Kyoto Encyclopedia of genes and genomes

International Union of Pure and Applied Chemistry

CI

AFP

Col-0

Co-2

Cvi

COR

DNA

Dehydrin

EI

DREB

F1, F2

ESI

GD

GC

hQTL

HPLC

A.thalianaaccession Landsbergerecta

A.thalianaaccessionCoimbra

Cold regulated

A.thalianaaccession Columbia

CRT binding factor

Cold acclimation specific

Chemical ionization

Calcium-dependent protein kinase

Atmospheric pressure photoionization

Anti- freeze proteins

A.thalianaaccession Canary Island

Best-parent-Heterosis

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Acclimated

Abcisic acid

Inducer of CBF expression

Effect- increased loci

Liquid chromatography

Late embryogenesis abundant

Genetic distance

High performance liquid chromatography

heterotic quantitative trait locus

independent component analysis

Electron Impact

Electron-Spray Ionization

Filial generation (first, second)

Gas chromatography

A.thalianaaccession Cape Verde Island

Dehydration induced

Desoxyribonucleic acid

Dehydration responsive element binding

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VII

Te

t f  TOF t R  TRAP

UV-light

US

sfr

SAR

TCA- cycle

SNP

RD

ROS

SA

Rsch

P

OE

QUAD

PAP

NIST

OD

MST

NA

MPH

MS

Mb

METB

MALDI

MAPK

LIFDI

LT 50  LTI

Mega base pairs

Abbreviations

Ion trap

Metabolite

United States

Ultraviolett light

Flow time

Time-of-flight

Retention time

Quadrupole detector

A.thalianaaccession Tenela

Responsive-to-dehydration

Salicylic acid

Tricarboxylic acid- cycle

Single nucleotide polymorphism

Sensitive to freezing

Systemic acquired resistance

A.thalianaaccession Rschew

Reactive oxygen species

Production of anthocyanin pigment

Liquid injection field desorption ionization

Lethal temperature

Low temperature induced

Mitogen activated protein kinase

Matrix- assisted laser desorption/ ionization

Mass spectrometry

Mid- parent-heterosis

National Institute of Standards and Technology

Mass spectral tags

Overdominance

Nonacclimated

Overexpression

Parental generation