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Publié par | heinrich-heine-universitat_dusseldorf |
Publié le | 01 janvier 2011 |
Nombre de lectures | 14 |
Langue | English |
Poids de l'ouvrage | 9 Mo |
Extrait
Mechanisms and Consequences of Particle Uptake
in Alveolar Macrophages
Inaugural-Dissertation
zur Erlangung des Doktorgrades
der Mathematisch-Naturwissenschaftlichen Fakultät
der Heinrich-Heine-Universität Düsseldorf
vorgelegt von
Agnes Martha Scherbart
aus Düren
Düsseldorf, Oktober 2010
aus dem Institut für Umweltmedizinische Forschung (IUF)
an der Heinrich-Heine Universität Düsseldorf gGmbH
Gedruckt mit der Genehmigung der
Mathematisch-Naturwissenschaftlichen Fakultät der
Heinrich-Heine-Universität Düsseldorf
Referentin: Prof. Dr. Christine R. Rose
Koreferentin: Prof. Dr. Charlotte Esser
Tag der mündlichen Prüfung: 01. Dezember 2010
Die Neugier steht immer an erster Stelle eines Problems, das gelöst werden will.
Galileo Galilei
15.02.1564 - 08.01.1642
italienischer Physiker und Astronom
To my parents Renata and Diethard Scherbart and
To my sister Eva-Maria Scherbart
TABLE OF CONTENT
Chapter I 1
1.1 Background - Where it all began 2
1.2 Nanoparticles - Benefits and Risks 4
1.2.1 The Particle Surface Properties - Reactive or Not 6
1.2.2 The Particle Size Distribution 8
1.2.3 The Ultrafine Hypothesis - A Question of Size 9
1.2.4 The Way of Exposure to Engineered Nanoparticles 9
1.3 The Lung - From Particle Inhalation to Pathogenesis 11
1.3.1 Particle Deposition in the Lung 12
1.3.2 The Clearance of Particles 13
1.4 Macrophages - Their Origin, Development, Differentiation & Function 15
1.5 Phagocytosis & Pinocytosis - Portals of Entry Into the Cell 16
1.5.1 Actin-Dependent Endocytosis 17
1.5.1.1 Phagocytosis 17
1.5.1.2 The Fc Receptor-Mediated Phagocytosis 17
1.5.1.3 Macropinocytosis 18
1.5.2 Actin-Independent Endocytosis 19
1.5.2.1 Clathrin-Mediated Endocytosis 19
1.5.2.2 Caveolae-Mediated Endocytosis 19
1.5.2.3 Clathrin- & Caveolin-Independent Endocytosis 19
1.6 Oxidative Stress - A Cell Fights Back 20
1.6.1 Cellular Generation of Reactive Oxygen / Nitrogen Species 20
1.6.2 Cellular Antioxidant Defence Mechanisms - Taking Care of Oneself 23
1.7 Inflammation & Oxidative Stress - Cellular Responses 25
1.7.1 Alveolar Macrophages - A Balance of Pro- & Anti-Inflammatory Actions 27
1.8 The Aim of the Thesis 29
1.9 References 31
I
?TABLE OF CONTENT
Chapter II 39
Contrasting Macrophage Activation by Fine and Ultrafine Titanium Dioxide Particles is
Associated with Different Uptake Mechanisms.
2.1. Introduction 42
2.2. Materials and Methods 45
2.3. Results 51
2.4. Discussion 64
2.5. References 69
Chapter I 75
Surface Iron Inhibits Quartz-Induced Cytotoxic and Inflammatory Responses in
Alveolar Macrophages.
3.1. Introduction 78
3.2. Materials and Methods 81
3.3. Results 88
3.4. Discussion 98
3.5. References 105
Chapter IV 113
Evaluation of Cytotoxic Effects and Oxidative Stress with Hydroxyapatite Dispersions
of Different Physicochemical Properties in Rat NR8383 Cells and Primary
Macrophages.
II TABLE OF CONTENT
Chapter V 127
5.1 Summary & General Discussion 129
5.2 References 135
5.3 Abstract 137
5.4 Zusammenfassung 138
5.5 List of Abbreviations 139
5.6 List of Publications 143
Danke an …
Eidesstattliche Erklärung
III TABLE OF CONTENT
IV