Structure, function analysis of the viral potassium channel Kcv [Elektronische Ressource] : mutagenesis studies of the two transmembrane domains / von Manuela Gebhardt

As Surques Escoeuilles

Description

Structure/Function Analysis of the Viral Potassium Channel Kcv Mutagenesis studies of the two transmembrane domains Vom Fachbereich Biologie der Technischen Universität Darmstadt zur Erlangung des akademischen Grades eines Doctor rerum naturalium genehmigte Dissertation von Dipl.-Biol. Manuela Gebhardt aus Berlin Berichterstatter: Prof. Dr. Gerhard Thiel Mitberichterstatter: Prof. Dr. Adam Bertl Eingereicht am 08.10.2010 Mündliche Prüfung am 10.12.2010 Darmstadt 2011 D17 1. Table of contents 1. ... Table of contents i 2. ... Chapter 1 - General Introduction 1 2.1. Ion Channels 1 2.1.1. Potassium Channels 1 2.1.2. Viral Potassium Channel Kcv 7 2.2. Interactions 9 2.2.1. Protein-Protein-Interactions 11 2.2.2. Protein-Lipid-Interaction 11 2.3. References 18 3. ...Chapter 2 - Computational Studies and Site Directed Mutagenesis reveals that Snorkeling Effects in the Viral Potassium Channel Kcv are nonessential for a proper Channel Function. 25 3.1. Abstract 25 3.2. Introduction 26 3.3. Material and Methods 28 3.3.1. Heterologous Expression Systems 28 3.3.2. Constructs and Mutagenesis 28 3.3.3. Electrophysiological Measurements 29 3.3.4. Saccharomyces cerevisiae Complementation Assay 30 3.3.5. Homology Model Structure Analysis 30 3.4. Results 31 3.5. Discussion 40 3.6. Conclusion 41 3.7. References 43 i 4. ...

Sujets

• Biologie

Informations

• Publié par: technischen_universitat_darmstadt
• Publié le: 01 janvier 2011
• Nombre de lectures: 29
• Langue: English
• Poids de l'ouvrage: 9 Mo

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