The content of olfactory memory in larval Drosophila [Elektronische Ressource] / Dushyant Mishra. Betreuer: Bertram Gerber

Dushyant

Sujets

• Biologie

Informations

• Publié par: julius-maximilians-universitat_wurzburg
• Publié le: 01 janvier 2012
• Nombre de lectures: 42
• Langue: Deutsch
• Poids de l'ouvrage: 6 Mo

Extrait

The content of olfactory memory in larval
Drosophila


Dissertation zur Erlangung des
naturwissenschaftlichen Doktorgrades
der Bayerischen Julius-Maximilians-Universität Würzburg


vorgelegt von

Dushyant Mishra
aus Saugor, M.P, Indien


Würzburg, 2011






1












Eingereicht am:
Mitglieder der Promotionskommission:
Vorsitzender:
Erster Gutachter:
Zweiter Gutachter:
Tag des Promotionskolloquiums:
Doktorurkunde ausgehändigt am:
2
Erklärung
gemäß § 4 Absatz 3 der Promotionsordnung der Fakultät für Biologie der
Bayerischen Julius-Maximilians-Universität zu Würzburg vom 15. März 1999:

Die vorgelegte Dissertation besteht aus drei Publikationen und zwei für die
Veröffentlichung vorbereiteten Manuskripten, sowie einer zusätzlichen „Allgemeinen
Einleitung und Diskussion“. Die Mitwirkung der Co-Autoren jeder Publikation werden
auf den folgenden Seiten dargestellt.

Ich versichere, dass ich diese Dissertation in allen Teilen selbständig angefertigt habe und
dazu keine anderen als die angegebenen Hilfsmittel benutzt habe.
Alle aus der Literatur entnommenen Textstellen sind als solche gekennzeichnet.

Die vorliegende Arbeit wurde weder in gleicher noch in ähnlicher Form bereits in einem
anderen Prüfungsverfahren vorgelegt. Zuvor habe ich keine akademischen Grade
erworben oder versucht zu erwerben.


Würzburg, den


Dushyant Mishra Prof. Dr. Bertram Gerber

3
Structure of the thesis
This thesis consists of 2 parts. Part I deals with a behavioural account of how intensity
and quality, the two defining features of an odour, are perceived in larval Drosophila. It
contains three chapters, corresponding to one manuscript prepared for publication and two
publications. The manuscript deals with odour intensity learning, the first publication
provides a behaviour based estimate of odour similarity in larval Drosophila, and the
second publication shows for two odours (3-octanol and 1-octene-3-ol) that perceptual
differences between these odours can either be ignored after non-discriminative training
(generalization), or accentuated by odour-specific reinforcement (discrimination).
Part II contains two chapters, corresponding to one manuscript prepared for publication
and one publication. The manuscript studies cognitive- enhancement in larval Drosophila
where I show that food supplementation with dried roots of Rhodiola rosea dose-
dependently improves odour- reward associative learning in larval Drosophila. The
publication deals with local loss of function of Synapsin and its consequences for
associative plasticity of larval Drosophila.
In addition, I present a ‘General Introduction and Discussion’ to give the reader an
overview of the background and implications of this thesis. Also included at the end is a
brief summary of the studies presented in this thesis.
This work would not have been possible without the effort of many people, and the
supervision of my mentor Dr. Bertram Gerber. I sincerely acknowledge the co- authors of
each manuscript, whose contributions are explicated below.










4
Chapter I.1

Mishra D., Chen Yi., Yarali A., Gerber B.: Olfactory memories can be intensity- specific
in larval Drosophila.
DM, BG and AY conceived the research and designed the experiments. DM and YC
performed the experiments and DM analyzed the data. DM, AY and BG wrote the paper.

Chapter I.2

Chen Yi., Mishra D., Schmitt L., Schmuker M., Gerber B. (2010): A behavioural odour-
similarity 'space' in larval Drosophila, Chemical Senses, 36(3), 237- 249.

YC, DM and BG conceived the research and designed the experiments. YC, LS and DM
performed the behaviour experiments. Specially, DM performed the experiments to
describe the dose effect curves for different odours which subsequently lead to adjust the
odour dilutions for equal learnability. YC, MS and BG analyzed the data. YC and BG
wrote the paper.


Chapter I.3

Mishra D., Louis M., Gerber B., (2010): Adaptive adjustment of the generalization-
discrimination balance in larval Drosophila, J. Neurogenetics, 24(3), 168-175.

DM, and BG conceived the research. DM and BG designed the experiments. DM
performed the experiments and analyzed the data. DM and BG wrote the paper with
inputs from ML.






5
Chapter II.1

= =Lushchak O. , Mishra D. , Haberkern H., Eschbach C., Lushchak V., Niewalda T., Gerber
K., Koblowsky M., Gerber B.: 'Cognitive enhancement’ of associative function in larval
Drosophila by Rhodiola rosea food supplementation.

OL, DM, VL and BG conceived the research. OL, DM and BG designed the experiments. , KG, MK performed the learning experiments concerning the larval Drosophila
and their controls. KG and TN helped to make the Rhodiola enriched fly food vials. CE
and HH performed and analyzed the larval tracking experiments. DM analyzed the data.
DM and BG wrote the paper.

Chapter II.2

Michels B., Chen Yi., Tanimoto H., Mishra D., Engmann O., Saumweber T., Gerber B.,
(2011): Site and mode of Synapsin action in associative learning, Learn. Mem. 18(5):332-
44.

BM, HT and BG conceived the research and designed the experiments. BM, OE and TS
performed the Synapsin rescue experiments. YC performed behavioural experiments with
PKA-site mutated transgenes. DM performed behavioural and immunohistochemical
analysis with Synapsin- RNAi lines. BM and BG wrote the paper.


Wuerzburg, den




Dushyant Mishra Prof. Dr. Bertram Gerber


6
Table of Contents

General introduction & discussion……………………..................…………................10

Part I
Olfactory percepts of larval Drosophila
I.1. How is odour intensity integrated into the odour percept?
Abstract……………………………………………..………..……………..….....35
Introduction.............................................................................................................36
Materials & Methods..............................................................................................39
Results.....................................................................................................................41
Discussion...............................................................................................................52
Acknowledgements.................................................................................................59
References...............................................................................................................59
Supplementary materials........................................................................................64

I.2. A behavioural odour similarity ‘space’
Abstract....................................................................................................................76
Introduction.............................................................................................................77
Materials & Methods..............................................................................................82
Results.....................................................................................................................86
Discussion...............................................................................................................95
Acknowledgements..................................................................................................99
7
References.............................................................................................................100
Supplementary materials.......................................................................................107

I.3. Odour percepts can be adaptively adjusted
Abstract.................................................................................................................115
Introduction...........................................................................................................116
Materials & Methods............................................................................................117
Results...................................................................................................................125
Discussion.............................................................................................................131
Acknowledgements................................................................................................133
References.............................................................................................................134
Supplementary material................................................................................