The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenine nucleotide translocator 2

Tanaka Michiko, Sata Tetsutaro, Kawaguchi, Kawaguchi Yasushi

Description

The herpes simplex virus 1 (HSV-1) UL7 gene is highly conserved among herpesviridae . Since the construction of recombinant HSV-1 with a mutation in the UL7 gene has not been reported, the involvement of HSV-1 UL7 in viral replication has been unclear. In this study, we succeeded in generating a UL7 null HSV-1 mutant virus, MT102, and characterized it. Our results were as follows. (i) In Vero cells, MT102 was replication-competent, but formed smaller plaques and yielded 10- to 100-fold fewer progeny than the wild-type virus, depending on the multiplicity of infection. (ii) Using mass spectrometry-based proteomics technology, we identified a cellular mitochondrial protein, adenine nucleotide translocator 2 (ANT2), as a UL7-interacting partner. (iii) When ANT2 was transiently expressed in COS-7 cells infected with HSV-1, ANT2 was specifically co-precipitated with UL7. (iv) Cell fractionation experiments with HSV-1-infected cells detected the UL7 protein in both the mitochondrial and cytosolic fractions, whereas ANT2 was detected only in the mitochondrial fraction. These results indicate the importance of HSV-1 UL7's involvement in viral replication and demonstrate that it interacts with ANT2 in infected cells. The potential biological significance of the interaction between UL7 and ANT2 is discussed.

Informations

• Publié par: biomed
• Publié le: 01 janvier 2008
• Nombre de lectures: 11
• Langue: English

Extrait

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Published: 22 October 2008 Received: 22 August 2008 Virology Journal 2008, 5 :125 doi:10.1186/1743-422X-5-125  Accepted: 22 October 2008 This article is available from: h ttp://www.virologyj.com/content/5/1/125 © 2008 Tanaka et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons. org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the orig inal work is properly cited.

Virology Journal

Abstract The herpes simplex virus 1 (HSV-1) UL 7 gene is highly conserved among herpesviridae . Since the construction of recombinant HSV- 1 with a mutation in the UL7 gene has not been reported, the involvement of HSV-1 UL7 in viral replication ha s been unclear. In this study, we succeeded in generating a UL7 null HSV-1 mutant virus, MT 102, and characterized it. Our results were as follows. (i) In Vero cells, MT102 was replication-competent, but formed smaller plaques and yielded 10- to 100-fold fewer progeny than the wild-type virus, depending on the multiplicity of infection. (ii) Using mass spectrometry-based proteomics technology, we iden tified a cellular mitochondrial protein, adenine nucleotide translocator 2 (ANT2) , as a UL7-interacting partner. (iii) When ANT2 was transiently expressed in CO S-7 cells infected with HSV-1, ANT2 was specifically co- precipitated with UL7. (iv) Cell fractionation experiments with HSV-1-in fected cells detected the UL7 protein in both the mitochondrial and cytoso lic fractions, whereas ANT2 was detected only in the mitochondrial fraction. Th ese results indicate the importan ce of HSV-1 UL7's involvement in viral replication and demonstrate that it inte racts with ANT2 in infe cted cells. The potential biological significance of the interacti on between UL7 and ANT2 is discussed.

Bio Med Central

Introduction conserved roles in the herpes virus life cycle. The viral gene Herpes simplex virus 1 (HSV-1) has a double-stranded is on the left side of the HSV-1 unique long (U L ) region DNA genome of about 152 kbp, from which more than 84 and surrounded by two essential viral genes (UL6 and ORFs are translated. Since Post and Roizman first charac- UL8) for virus replication in cell cultures [3]. The UL7 terized recombinant viruses in which a specific HSV-1 gene partially overlaps with the UL6 gene, and these tran- gene was mutated by the reverse genetics system [1], this scripts are coterminal at their 3' ends. Information on the gene's roles in the viral life cycle have been extensively function(s) of the HSV UL7 gene product in the viral life investigated. By now, there remain only a handful of HSV- cycle is limited. The only reported experimental evidence 1 genes whose roles have not been investigated using a with regard to HSV UL7 is that its gene products are recombinant virus with a mutated gene. The UL7 gene, the present in integumentary layers of mature virions, and subject of this study, is one such viral gene. The UL7 that the viral protein is localized predominantly in the amino acid sequence is conserved in all Herpesviridae sub- juxtanuclear cytoplasmic domains of infected cells, families [2], suggesting that UL7 homologues may play although it is also detected transiently in the nucleus [4].

Address: 1 Department of Pathology, National Institute of Infe ctious Diseases, Shinjuku-k u, Tokyo 162-8640, Japan and 2 Department of Infectious Disease Control, International Re search Center for Infectious Disease, The Instit ute of Medical Science, The University of Toky o, Tokyo 108-8639, Japan Email: Michiko Tanaka* - [e-mail]; Tetsutaro Sata - [e-mail]; Yasu shi Kawaguchi - [e-mail] * Corresponding author

Research Open Access The product of the Herpes simplex virus 1 UL7 gene interacts with a mitochondrial protein, adenin e nucleotide translocator 2 Michiko Tanaka* 1 , Tetsutaro Sata 1 and Yasushi Kawaguchi 2