The role of protein-protein interactions in the activation cycle of RAF kinases [Elektronische Ressource] / vorgelegt von Andreas Fischer

Description

The Role of Protein-Protein Interactions in the Activation Cycle of RAF Kinases Dissertation zur Erlangung desnaturwissenschaftlichen Doktorgradesder Julius-Maximilians-Universität Würzburg vorgelegt von Andreas Fischer aus Neuburg an der Donau Würzburg 2010Eingereicht am: Mitglieder der Promotionskommission:Vorsitzender: Prof. Dr. Thomas DandekarGutachter : Prof. Dr. Ulf R. RappGutachter: Prof. Dr. Roland Benz Tag des Promotionskolloquiums: Doktorurkunde ausgehändigt am: Für meine ElternTable of Contents Summary.............................................................................................................................................7 Zusammenfassung..........................................................................................................................91. Introduction.......................................................................................................................................11 1.1 Protein Phosphorylation and Protein-Protein Interaction – Essential Tools for Signaling Networks......................................................................12 1.1.1 Protein Phosphorylation....................................................................................12 1.1.2 Protein-Protein Interactions.............................................................................13 1.2 The MAP Kinase Signaling Cascade..............................................................................15 1.

Sujets

• Biologie

Informations

• Publié par: julius-maximilians-universitat_wurzburg
• Publié le: 01 janvier 2010
• Nombre de lectures: 11
• Langue: English
• Poids de l'ouvrage: 3 Mo

Extrait

The Role of Protein-Protein Interactions in the Activation Cycle of RAF Kinases  

 

Dissertation zur Erlangung des

naturwissenschaftlichen Doktorgrades

der Julius-Maximilians-Universität Würzburg

 

vorgelegt von

   Andreas Fischer

aus Neuburg an der Donau    

Würzburg 2010

Eingereicht am:

 

Mitglieder der Promotionskommission:

Vorsitzender: Prof. Dr. Thomas Dandekar

Gutachter : Prof. Dr. Ulf R. Rapp

Gutachter: Prof. Dr. Roland Benz

 

Tag des Promotionskolloquiums:  

Doktorurkunde ausgehändigt am:

Für mein e Eltern

    1.                             2.             3.       4. 5.            

Table of Contents

Summary........7..................................................................................................................................... Zusammenfassung.....................................................................................9..................................... Introduction....................................................11................................................................................... 1.1 Protein Phosphorylation and Protein-Protein Interaction –  Essential Tools for Signaling Networks......................................................................12  1.1.1 Protein Phosphorylation....................................................................................12  1.1.2 Protein-Protein Interactions.............................................................................13 1.2 The MAP Kinase Signaling Cascade..............................................................................15  1.2.1 RAF Kinases.............................................................................................................17  1.2.2 Ras – The Path is the Goal ..................................................................................21  1.2.3 Ras – RAF Interaction...........................................................................................23  1.2.4 Prohibitin....................................................................................................................24  1.2.5 The RAF Substrate MEK......................................................................................25  1.2.6 KSR – more than a scaffold................................................................................25  1.2.7 The MAP Kinase ERK...........................................................................................26  1.2.8 14-3-3Proteins........................................................................................................27  1.2.9 Current Model of RAF Activation....................................................................30 Scientiic work...................................................................................................33................................ 2.1B- and C-RAF display essential differences in their binding to Ras:  The Isotype Speciic N-terminus of B-RAF Facilitates Ras Binding....................35 2.2Regulation of RAF activity by 14-3-3 proteins: RAF kinases associate  functionally with both homo- and heterodimeric forms of 14-3-3 proteins...55 2.3Novel C-Raf phosphorylation sites: serine 296 and 301 participate in  Raf regulation.........................................................................................................................37 Discussion................1.8........................................................................................................................... 3.1 Ras and RAF – Tying up for Activation........................................................................82 3.2 14-3-3 proteins – Adaptors for the (conformational) change we need........84 3.3 Fine tuning by feedback....................................................................................................87 References........19.......................................................................................................................... Appendix.......................................................................................................................................1.30.... 5.1 Abbreviations.....................................................................................................................103 5.2 List of Publications...........................................................................................................105 5.3 Poster Abstracts.................................................................................................................106 5.4  Erklärung..............................................................................................................................107 5.5 Curriculum   ....e...Vita................................................................................................801......... 5.6 Danksagung..........................................................................................................................109

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