FITMASTER TUTORIAL

Heka Elektronik

Informations

• Publié par: Epsu
• Nombre de lectures: 272
• Langue: English

Extrait

Tutorial 2.20
FITMASTER
Tutorial©
HEKA Elektronik Phone [phone] / 95 53-0
Dr. Schulze GmbH Fax [phone] / 95 53-50
Wiesenstrasse 71 Web Site www.heka.com
D-67466 Lambrecht/Pfalz Email [e-mail]
Germany [e-mail]
HEKA Electronics Inc. Phone [phone]
47 Keddy Bridge Road Fax [phone]
R.R. #2 Web Site www.heka.com
Mahone Bay, NS B0J 2E0 Email [e-mail]
Canada [e-mail]
HEKA Instruments Inc. Phone [phone]
2128 Bellmore Avenue Fax [phone]
Bellmore, New York 11710-5606 Web Site www.heka.com
USA Email [e-mail]
[e-mail]
Title Page: Blood Vessels of the Retina; courtesy of Max-Planck-Institute for Brain
Research, Frankfurt
2004-2010 HEKA Elektronik Dr. Schulze GmbH
COFITU/1Contents
1 Introduction 1
1.1 Prerequisites . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 Window Placement . . . . . . . . . . . . . . . . . . . . . . . 2
1.3 Start-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.4 Hints and Tips . . . . . . . . . . . . . . . . . . . . . . . . . 3
2 Analysis and Fit of a Current-Voltage Relationship 5
3 Demonstration of Background Traces 7
4 Averaging results from different Series 9
4.1 Export SeriesFit results with error bars in IgorPro format . 10
5 Analysis of a Recovery Process and Fit of Exponential 11
5.1 Fitting of an exponential curve to the data . . . . . . . . . 13
5.2 Inspection of data by mouse click . . . . . . . . . . . . . . . 13
6 Analysis of a Time Series 15
6.1 Normalization to baseline results . . . . . . . . . . . . . . . 15
6.2 Normalization to baseline results, using Online Analysis . . 16
6.3 Adapting the Graph display . . . . . . . . . . . . . . . . . . 17
6.4 Analyzing the current reduction . . . . . . . . . . . . . . . . 18
7 Cyclic Analysis 21ii CONTENTS
7.1 Normalizing each inactivation curve . . . . . . . . . . . . . 22
7.2 Averaging normalized inactivation curves and fitting an ex-
ponential . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
8 Amplitude Histograms 25
9 Power Spectra Analysis 27
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1. Introduction
1.1 Prerequisites
For demonstrating FitMaster, please
1. InstallFitMasterversion2.05fromtheHEKACD-ROMordown-
load a web installer from our web site.
The installation contains:
a default Online Analysis file DefAnalFit.onl
a default Key file FitMaster.key
a default setting file FitMaster.set
all located in the Fitmaster folder.
2. DemoDataInaddition,weprovideademodatasetfortheFitmas-
ter program. In case you use downloaded Fitmaster from out web
site, please also download the zip file containing demo data. Please
copy the file DemoFitData in the C:/HEKA/Data folder.
This DemoFitData data set includes the following series:
IV
Hinf (3 Series)
Recovery
Onrate
Trains (3 Series)
3. Dongle A dongle is requires if you would like to save your analysis
results. The demo data set can be loaded without a dongle.ˆ
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2 Introduction
1.2 Window Placement
For demonstrating FitMaster you just need to display the following win-
dows:
TraceFit
Oscilloscope
Replay
OnlineAnalysis
Notebook
We recommend the following window placement.
Figure 1.1: Recommended window placement for demonstrating FitMas-
ter
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1.3 Start-up 3
1.3 Start-up
Double click the Fitmaster program executable and start the demonstra-
tion with using Fitmaster’s default settings.
Then, load the file DemoFitData.dat in Modify Analysis mode. Please
double check if the default Online Analysis is loaded DefAnalFit.onl.
The OnlineAnalysis should be set to Automatic Stimulus Control.
This way the default analysis is always selected correctly and if you want
you do not have to worry about the online analysis at all.
1.4 Hints and Tips
Please note the following:
Theanalysisfile DemoFitData.anaifpresentfromapreviousdemon-
stration should be deleted or renamed. Starting the demonstration
withanemptyanalysisfilehelpskeepinganoverviewonalltheresults
generated.
A major difficulty is to navigate through your analysis results in
SeriesFit. Parameters might have the same name for different Se-
ries. Therefore, please always have a look at the ”Series” selection in
the SeriesFit window (top left).
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2. Analysis and Fit of a
Current-Voltage Relationship
Open the TraceFit window (or activate it).
Set the FitType to OnlineOnly.
In the Replay window select the first sweep of the IV series.
Click Auto Fit in the TraceFit window. (IV parameters are ana-
lyzed.)
Go to the SeriesFit window.
Selectfromtheparameters“Ampl”inthefirstrowand“Extr”inthe
second row.
Make sure that Parameters from Results is selected.
Press Preview in the waves section to display the data in the
SeriesGraph.
Select Current-Voltage function from the Fit Type selection and
press Fit.6 Analysis and Fit of a Current-Voltage Relationship
Figure 2.1: SeriesGraph: Results and Fit of an IV curve.
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