A comparative study of seven in-house and two laboratory hematology instruments [Elektronische Ressource] / eingereicht von Martina Becker

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• Publié par: justus-liebig-universitat_giessen
• Publié le: 01 janvier 2008
• Nombre de lectures: 20
• Langue: English
• Poids de l'ouvrage: 2 Mo

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A COMPARATIVE STUDY OF SEVEN IN-HOUSE AND
TWO LABORATORY HEMATOLOGY INSTRUMENTS
MARTINA BECKER
INAUGURAL-DISSERTATION
zur Erlangung des Grades eines
Dr. med. vet.
beim Fachbereich Veterinärmedizin
édition scientifique der Justus-Liebig-Universität Gießen
VVB LAUFERSWEILER VERLAG

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VVB VVB LAUFERSWEILER VERLAG
COMPARATIVE STUDY OF HEMATOLOGY INSTRUMENTS MARTINA BECKER Das Werk ist in allen seinen Teilen urheberrechtlich geschützt.
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1. Auflage 2008
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édition scientifique
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www.doktorverlag.deAus der Klinik für Kleintiere, klinische Pathophysiologie und klinische
Laboratoriumsdiagnostik

Betreuer: Prof. Dr. Andreas Moritz



A Comparative Study of
Seven In‐House and
Two Laboratory
Hematology Instruments








INAUGURAL-ISSERTATION
zur Erlangung des Grades eines
Dr. med. vet.
beim Fachbereich Veterinärmedizin
der Justus-Liebig-Universität Gießen








eingereicht von


Martina Becker

Tierärztin aus Madrid/Spanien




Gießen, 2007




Mit Genehmigung des Fachbereichs Veterinärmedizin
der Justus-Liebig-Universität Gießen.




Dekan: Prof. Dr. Dr. G. Baljer









Gutachter:

Prof. Dr. A. Moritz

Prof. Dr. A. Wehrend










Tag der Disputation: 21.12.2007 Content 
1. Introduction and Literature Overview.................................................................................. 9
1.1. Method validation .................................................................................................................... 10
1.1.1. Linearity experiment.......................................................................................................................... 12
1.1.2. Replication experiment...................................................................................................................... 12
1.2.3. Method comparison ....................................................................................................... 13
1.2.4. Judgment of method performance ..................................................................................................... 17
2. Material and Method.. 20
2.1. Sample specifications ............................................................................................................... 20
2.2 Evaluated instruments.............................................................................................................. 24
2.2.1. Instrument A (Cell-Dyn 3500)........................................................................................................... 24
2.2.2. Instrument B (ADVIA™ 120)........................................................................................................... 26
2.2.3. Instrument C (LaserCyte®)................ 27
2.2.4. Instrument D (ForCyte) ..................................................................................................................... 30
2.2.5. Instrument E (MS45)......................................................................................................................... 31
2.2.6. Instrument F (Heska CBC) ................................................................................................................ 32
2.2.7. Instrument G (Scil Vet ABC) ............................................................................................................ 33
2.2.8. Instrument H (VetScan HMT)........................................................................................................... 34
® TM2.2.9. Instrument K (QBC Vet Autoread ) 35
2.3 Statistics ..................................................................................................................................... 36
2.3.1 Cell count............................................................................................................................................ 36
2.3.2 Differential ......................................................................................................................................... 37
2.3.3 Replication experiment....................................................................................................................... 37
2.3.4 Linearity experiment........................................................................................................................... 38
2.3.5 Carry-over experiment........................................................................................................................ 38
3. Results.................................................................................................................................. 39
3.1 Instrument Specifications and IT-options .............................................................................. 39
3.2 Flags ........................................................................................................................................... 40
3.2.1 Blood Cell count................................................................................................................................. 40
3.2.2 Differential blood cell count............................................................................................................... 45
3.3 Precision, Linearity and Carryover......................................................................................... 50
3.4 Accuracy .................................................................................................................................... 51
7 3.4.1 Cell count............................................................................................................................................ 51
3.4.2 Differential blood cell count............................................................................................................... 70
4. Discussion............................................................................................................................ 80
4.1 Accuracy .................................................................................................................................... 80
4.2 Instrument flags ........................................................................................................................ 88
4.3 Precision, Linearity and Carryover......................................................................................... 90
4.4 Ease of use.................................................................................................................................. 91
5. Summary.............................................................................................................................. 92
6. Zusammenfassung............................................................................................................... 95
7. Reference List...................................................................................................................... 99
8. Abbreviations..................................................................................................................... 106
9. Figures.......... 108
10. Tables.......... 111
11. Acknowledgments............................................................................................................ 114
 
 
8  
1. Introduction  
               and Literature Overview 
The evaluation of blood has become an integral part in the routine clinical 
assessment of healthy and diseased companion animals. The packed cell volume 
(PCV), or as in humans the total hemoglobin concentration, and the microscopic 
examination of a blood smear have been used since the beginning of veterinary care 
and still&