Antimicrobial activity of some sulfonamide derivatives on clinical isolates of Staphylococus aureus

Genç Yeliz, Özkanca Reåÿit, Bekdemir, Bekdemir Yunus

Description

Staphylococcus aureus is a non-motile, gram positive, non-sporforming, facultative anaerobic microorganism. It is one of the important bacteria as a potential pathogen specifically for nosocomial infections. The sulfonamide derivative medicines are preferred to cure infection caused by S. aureus due to methicillin resistance. Methods Antimicrobial activity of four sulfonamide derivatives have been investigated against 50 clinical isolates of S. aureus and tested by using MIC and disc diffusion methods. 50 clinical isolate which collected from specimens of patients who are given medical treatment in Ondokuz Mayis University Medical School Hospital. A control strain of S. aureus ATCC 29213 was also tested. Results The strongest inhibition was observed in the cases of I [ N -(2-hydroxy-4-nitro-phenyl)-4-methyl-benzensulfonamid], and II [ N -(2-hydroxy-5-nitro-phenyl)-4-methyl-benzensulfonamid] against S. aureus . Compound I [ N -(2-hydroxy-4-nitro-phenyl)-4-methyl-benzensulfonamid] showed higher effect on 21 S. aureus MRSA isolates than oxacillin antibiotic. Introducing an electron withdrawing on the ring increased the antimicrobial activity remarkably. Conclusion This study may help to suggest an alternative possible leading compound for development of new antimicrobial agents against MRSA and MSSA resistant S. aureus . It was also shown here that that clinical isolates of 50 S. aureus have various resistance patterns against to four sulfonamide derivatives. It may also be emphasized here that in vitro antimicrobial susceptibility testing results for S. aureus need standardization with further studies and it should also have a correlation with in vivo therapeutic response experiments.

Informations

• Publié par: biomed
• Publié le: 01 janvier 2008
• Nombre de lectures: 23
• Langue: English

Extrait

Annals of Clinical Microbiology and Antimicrobials

BioMedCentral

Open Access Research Antimicrobial activity of some sulfonamide derivatives on clinical isolates of Staphylococus aureus 1 12 YelizGenç,Re¸sitÖzkanca*andYunusBekdemir

1 2 Address: Departmentof Biology, Faculty of Science and Arts, Ondokuz Mayis University, Kurupelit, 55139, Samsun, Turkey andDepartment of Chemistry, Faculty of Science and Arts, Ondokuz Mayis University, Kurupelit, 55139, Samsun, Turkey Email: Yeliz Genç  [e-mail]; Res¸it Özkanca*  [e-mail]; Yunus Bekdemir  [e-mail] * Corresponding author

Published: 20 August 2008Received: 7 March 2008 Accepted: 20 August 2008 Annals of Clinical Microbiology and Antimicrobials2008,7:17 doi:10.1186/1476-0711-7-17 This article is available from: http://www.ann-clinmicrob.com/content/7/1/17 © 2008 GENÇ et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:Staphylococcus aureusis a non-motile, gram positive, non-sporforming, facultative anaerobic microorganism. It is one of the important bacteria as a potential pathogen specifically for nosocomial infections. The sulfonamide derivative medicines are preferred to cure infection caused byS. aureusdue to methicillin resistance. Methods:Antimicrobial activity of four sulfonamide derivatives have been investigated against 50 clinical isolates ofS. aureusand tested by using MIC and disc diffusion methods. 50 clinical isolate which collected from specimens of patients who are given medical treatment in Ondokuz Mayis University Medical School Hospital. A control strain ofS. aureusATCC 29213 was also tested. Results:The strongest inhibition was observed in the cases ofI[N-(2-hydroxy-4-nitro-phenyl)-4- methyl-benzensulfonamid], andII[N-(2-hydroxy-5-nitro-phenyl)-4-methyl-benzensulfonamid] againstS. aureus. CompoundI[N-(2-hydroxy-4-nitro-phenyl)-4-methyl-benzensulfonamid] showed higher effect on 21S. aureusMRSAisolates than oxacillin antibiotic. Introducing an electron withdrawing on the ring increased the antimicrobial activity remarkably. Conclusion:This study may help to suggest an alternative possible leading compound for development of new antimicrobial agents against MRSA and MSSA resistantS. aureus. It was also shown here that that clinical isolates of 50S. aureushave various resistance patterns against to four sulfonamide derivatives. It may also be emphasized here that in vitro antimicrobial susceptibility testing results forS. aureusneed standardization with further studies and it should also have a correlation with in vivo therapeutic response experiments.

Background Staphylococcus aureusis one of the most significant human pathogens responsible for nosocomial and community acquired infections. It can cause a range of infectious dis ease from mild conditions, such as soft tissue infections, to severe lifethreatening debiliation, such as endocarditis

[1]. Despite the recent staphylococci infections, they are persisting as an important hospital and community path ogen [2]. Methicillin resistance has become a major con cern to the medical community due to the fact that they have an extraordinary ability to adapt rapidly to antibiotic stress [3]. Among hospital isolates the frequency of methi

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