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Publié par | humboldt-universitat_zu_berlin |
Publié le | 01 janvier 2008 |
Nombre de lectures | 19 |
Langue | English |
Poids de l'ouvrage | 4 Mo |
Extrait
Humboldt Universität zu Berlin Leibniz‐Institute für
Landwirtsschaftlich‐ Gärtnerische Fakultät Gewässerökologie und Binnenfischerei
Characterisation and host‐parasite
interaction of the piscine diplomonad
Spironucleus salmonis
Mohammad Reza Saghari Fard
July, 2008
Titel der Arbeit:
Characterisation and host‐parasite interaction of the piscine
diplomonad Spironucleus salmonis
D i s s e r t a t i o n
zur Erlangung des akademischen Gradedoctor rerum agriculturarum
(Dr. rer. agr.)
eingereicht an der
Landwirtschaftlich‐Gärtnerischen Fakultät
der Humboldt‐Universität zu Berlin
von
Mohammad Reza Saghari Fard
15 March 1971, Mashad, Iran
Präsident der Humboldt‐Universität zu Berlin
Prof. Dr. Dr. h.c. Christoph Markschies
Dekan der Landwirtschaftlich‐Gärtnerischen Fakultät:
Prof. Dr. Dr. h.c. Otto Kaufmann
Gutachter:
1. Prof. Dr. Frank Kirschbaum
2. Dr. Sarah L. Poynton
3. Dr. Klaus Knopf
Tag der Verteidigung: 21 November 2008
I would like to dedicate this thesis to my loving parents….
CONTENTS
DECLARATION................................................................................................................................................... 8
ACKNOWLEDGMENTS .................................................................................................................................... 9
PAPERS............................................................................................................................................................... 11
CONTRIBUTIONS TO PAPERS ..................................................................................................................... 13
CONFERENCE PRESENTATIONS ................................................................................................................ 15
DISSERTATION OVERVIEW......................................................................................................................... 17
ABSTRACT......................................................................................................................................................... 19
CHAPTER 1 OVERVIEW OF DIPLOMONADS........................................................................................... 22
1.1 Taxonomy................................................................................................................................................... 22
1.2 Phylogeny ................................................................................................................................................... 24
1.3 Morphology and ultrastructure ................................................................................................................... 28
1.4 Habitat ........................................................................................................................................................ 31
1.5 Life cycle - reproduction............................................................................................................................. 33
1.6 Pathology.................................. 34
1.7 Immunity................................. 36
1.8 Management and treatment of infection ..................................................................................................... 37
CHAPTER 2 PISCINE DIPLOMONAD: SPIRONUCLEUS ......................................................................... 40
2.1 Aquaculture and Spironucleus.................................................................................................................... 40
2.2 Identification of Spironucleus species in fish - chronology........................................................................ 41
2.3 Habitat of Spironucleus .............................................................................................................................. 46
2.4 Life cycle of Spironucleus – trophozoite and cyst 48
2.5 Encystment of Spironucleus – the role of flagella ...................................................................................... 49
2.6 Pathology associated with Spironucleus..................................................................................................... 52
2.7 Innate immunity to Spironucleus................................................................................................................ 56
2.8 Acquired immunity..................................................................................................................................... 58
CHAPTER 3 SPECIFIC AIMS ......................................................................................................................... 61
3.1 Overview .................................................................................................................................................... 61
3.2 Characterisation of Spironucleus 62
3.3 Intestinal pH and the microhabitat preference of Spironucleus .................................................................. 63
3.4 Encystment of Spironucleus – the role of flagella ...................................................................................... 64
3.5 Pathology associated with Spironucleus..................................................................................................... 65
3.6 Innate immunity to Spironucleus – in vitro plasma incubation test............................................................ 67
4CHAPTER 4 MATERIALS AND METHODS ................................................................................................ 70
4.1 Source of fish.............................................................................................................................................. 70
4.2 Wet preparations of diplomonads ............................................................................................................... 70
4.3 Characterisation of Spironucleus ................................................................................................................ 71
4.3.1 Ultrastructural characterisation ......................................................................................................... 71
4.3.2 Molecular characterisation................................................................................................................. 73
4.4 Intestinal pH and the microhabitat preference of Spironucleus .................................................................. 74
4.4.1 Data collection .................................................................................................................................... 74
4.4.2 Statistical analysis..................................................................................................... 75
4.5 Encystment – the role of Spironucleus flagell