Characterization of the interactions and enzyme activities of the type IV secretion components VirB1 and VirB11 from Brucella suis and Agrobacterium tumefaciens [Elektronische Ressource] / vorgelegt von Christoph Höppner

Höppner, Christoph

Description

Characterization of the Interactions and EnzymeActivities of the Type IV Secretion ComponentsVirB1 and VirB11 fromBrucella suis and Agrobacterium tumefaciensDissertationder Fakultät für Biologie der Ludwig-Maximilian-Universität Münchenvorgelegt vonChristoph Höppneram 3. Februar 20051. Gutachter: Prof. Dr. Christian Baron2. Gutachter: Prof. Dr. August BöckTag der mündlichen Prüfung: 22. März 2005INTRODUCTION 11. A successful mechanism used for diverse applications 12. Similarity between the A. tumefaciens and B. suis T4SS 33. A dual role of the soluble lytic transglycosylase VirB1 73.1. The catalytic mechanism of SLTs 73.2. VirB1 interacts with T4SS components 94. The VirB11-like hexameric (d)NTPase of T4SS 94.1. General characteristics and role in the system 94.2. Choice of VirB11 orthologs as drug targets 115. Social and economic impact of some T4SS-mediated diseases 136. Aim of this work 18MATERIALS AND METHODS 191. Bacterial strains, bacteriophages and plants 191.1. Bacterial strains 191.2. Bacteriophages and plants 202. Plasmids and genomic DNA 203. Oligonucleotides 253.1. PCR primers 253.2. Site-directed mutagenesis primers 273.3. Specialized sequencing primers 274. Growth media, supplements and antibiotics 284.1. Growth media 284.2. Supplements, antibiotics and inhibitors 295. Buffers and solutions 306. Growth conditions 306.1. Escherichia coli 306.2. Agrobacterium tumefaciens 317. Photometric methods 317.1.

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• Biologie

Informations

• Publié par: ludwig-maximilians-universitat_munchen
• Publié le: 01 janvier 2005
• Nombre de lectures: 24
• Langue: English
• Poids de l'ouvrage: 1 Mo

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