Cytoskeleton assembly at endothelial cell-cell contacts is regulated by {αII-spectrin-VASP [alpha-II-spectrin-VASP] complexes [Elektronische Ressource] / vorgelegt von Peter Michael Benz

Benz_P

Description

CYTOSKELETON ASSEMBLY AT ENDOTHELIAL CELL-CELL CONTACTS IS REGULATED BY αII-SPECTRIN/VASP COMPLEXES DISSERTATION ZUR ERLANGUNG DES NATURWISSENSCHAFTLICHEN DOKTORGRADES DER BAYERISCHEN JULIUS-MAXIMILIANS-UNIVERSITÄT WÜRZBURG vorgelegt von Peter Michael Benz aus Scherzingen Würzburg 2007 Eingereicht am: _____________________ bei der Fakultät für Chemie und Pharmazie. 1. Gutachter: Prof. Dr. Ulrich Walter 2. Gutachter: Prof. Dr. Utz Fischer der Dissertation. 1. Prüfer: Prof. Dr. Ulrich Walter 2. Prüfer: Prof. Dr. Utz Fischer 3. Prüfer: PD. Dr. Thomas Renné des öffentlichen Promotionskolloquiums. Tag des öffentlichen Promotionskolloquiums: _________________________________ Doktorurkunde ausgehändigt am: __________________________________________ Die vorliegende Arbeit wurde in der Zeit von Februar 2004 bis Mai 2007 in der DFG Nachwuchsgruppe von PD. Dr. Thomas Renné am am Institut für Klinische Biochemie und Pathobiochemie der Bayerischen Julius-Maximilians-Universität Würzburg angefertigt. Teile der im Rahmen dieser Arbeit erzielten Ergebnisse sind bereits veröffentlicht worden: Publikationen Benz, P.M., Blume, C., Moebius, J., Oschatz, C., Schuh, K., Sickmann, A., Walter, U., Feller S. M., and Renné, T. (2007) Cytoskeleton assembly at endothelial cell-cell contacts is regulated by αII-spectrin/VASP complexes.

Sujets

• Biologie

Informations

• Publié par: julius-maximilians-universitat_wurzburg
• Publié le: 01 janvier 2007
• Nombre de lectures: 36
• Langue: Deutsch
• Poids de l'ouvrage: 3 Mo

Extrait

CYTOSKELETON ASSEMBLY AT ENDOTHELIAL CELL-CELL CONTACTS IS REGULATED BY αII-SPECTRIN/VASP COMPLEXES    

      DISSERTATION ZUR ERLANGUNG DES NATURWISSENSCHAFTLICHEN DOKTORGRADES DER BAYERISCHEN JULIUS-MAXIMILIANS-UNIVERSITÄT WÜRZBURG             vorgelegt von     Peter Michael Benz       aus Scherzingen             Würzburg 2007

                          Eing _____________________ ereicht am:

bei der Fakultät für Chemie und Pharmazie.

 

 

1. Gutachter: Prof. Dr. Ulrich Walter

2. Gutachter: Prof. Dr. Utz Fischer

der Dissertation.

 

 

1. Prüfer: Prof. Dr. Ulrich Walter

2. Prüfer: Prof. Dr. Utz Fischer

3. Prüfer: PD. Dr. Thomas Renné

des öffentlichen Promotionskolloquiums.

 

 

Tag des öffentlichen Promotionsko quiums: _________________________________ llo

 

 

Doktorurkunde ausgehändigt am:   __________________________________________

 

 

 

Die vorliegende Arbeit wurde in der Zeit von Februar 2004 bis Mai 2007

in der DFG Nachwuchsgruppe von PD. Dr. Thomas Renné am

am Institut für Klinische Biochemie und Pathobiochemie

der Bayerischen Julius-Maximilians-Universität Würzburg angefertigt.

                  Teile der im Rahmen dieser Arbeit erzielten Ergebnisse sind bereits veröffentlicht worden:    Publikationen Benz, P.M., Blume, C., Moebius, J., Oschatz, C., Schuh, K., Sickmann, A., Walter, U., Feller S. M., and Renné, T. (2007) Cytoskeleton assembly at endothelial cell-cell contacts is regulated by αII-spectrin/VASP complexes.J Cell Biol. In revision.   Benz, P.M., Feller S.M., Walter, U., and Renné, T. (2007) Prostaglandin-induced VASP phosphorylation controlsαII-Spectrin break down in apoptotic cells.Int Immunopharmacol.In press.  

Blume, C., Benz, P.M., Walter, U., Ha, J., Kemp, B.E., and Renné, T. (2007) AMP-activated Protein Kinase Impairs Endothelial Actin Cytoskeleton Assembly by Phosphorylating Vasodilator-stimulated Phosphoprotein.J Biol Chem, 282, 4601-4612.  

Johne, J., Blume, C., Benz, P.M., Pozgajova, M., Ullrich, M., Schuh, K., Nieswandt, B., Walter, U., and Renné, T. (2006) Platelets promote coagulation factor XII-mediated proteolytic cascade systems in plasma.Biol Chem, 387, 173-178.

Posterpräsentationen und Vorträge Benz, P.M., Blume, C., Schuh, K., Feller, S. M., Sickmann, A., Walter, U., and Renné, T. The cAMP-dependent Interaction of Vasodilator-stimulated phosphoprotein (VASP) with alpha-II Spectrin Regulates Cortical Cytoskeleton Formation in Endothelial Cells. Poster bei der 3. Jahrestagung der Deutschen Vereinten Gesellschaft für Klinische Chemie und Laboratoriumsmedizin, Mannheim, Germany, October 1-4.2006.   Benz, P.M., Blume, C., Schuh, K., Feller, S. M., Sickmann, A., Walter, U., and Renné, T. Interaction of VASP withαII-Spectrin is Crucial for the cAMP-Dependent Regulation of Cortical Actin Dynamics. SFB 688 Symposium, Würzburg, Germany, Mai 18 th 2006.   Benz, P.M., Schuh, K., Blume, C., Feller, S.M., Sickmann, A., Walter, U., and Renné, T. Interaction of Vasodilator-stimulated phosphoprotein (VASP) withαII-Spectrin is Crucial for the cAMP-Dependent Regulation of Cortical Actin Dynamics. Poster at the 100th Annual Meeting of the American Society for Biochemistry and Molecular Biology, San Francisco, U.S.A., April 1- 5.2006. Abstract inFASEB J. 2006; 20:A103.    Blume, C., Schuh, K., Benz, P.M., Walter, U., and Renné, T. In vivo modulation of vasodilator stimulated phosphoprotein functions by phosphorylation. International Conference of cGMP Generators, Effectors and Therapeutic Implications, Potsdam, Germany. June 10–12. 2005. Abstract inBMC Pharmacol.2005; 5 (Suppl 1), P4.   Blume, C., Schuh, K., Benz, P.M., Walter, U., and Renné, T. Differential phosphorylations of the vasodilator stimulated phosphoprotein (VASP) regulate actin-dynamics and subcellular targeting. 46th Spring Meeting of the German Society of Experimental and Clinical Pharmacology and Toxicology, Mainz, March 15-17.2005. Abstract in Naunyn-Schmiedebergs Archives of Pharmacology. 2005; 371, R50.   

 

 

I would like to thank ...   Prof. Dr. Ulrich Walter for giving me the possibility to do my PhD research in his institute, for his constant interest in my work, and the opportunity to present my results at the "Centennial Meeting of the American Society of Biochemistry and Molecular Biology (ASBMB)" in San Francisco, April 2006.   Prof. Dr. Utz Fischer who as a member of the Faculty of Chemistry and Pharmacology kindly accepted to examine this thesis.   PD. Dr. Thomas Rennè for his continuous support of my project, the excellent working conditions, and the freedom to develop my own ideas and solutions to a problem.   Prof. Dr. Kai Schuh for his patience answering all my "hypothetical" questions, the helpful discussion of experimental problems and results, and the legendary meetings at Burger King.   Constanze Blume for the never-ending supply of VASP-mutants and her logistic skills, which made the daily lab-life so much easier.   Dr. Albert Sickmann and Dr. Jan Moebius for the mass spectrometry-based identification of VASP-binding proteins.   Dr. Stephan Feller for his help with all SH3-related problems, the peptide scan arrays, and the critical and time-consuming discussion of our manuscript.   All members of the institute who assisted me and who contributed to the very pleasant working atmosphere. In particular I thank Monika Kuhn for help with VASP affinity columns, Melanie Ullrich for help with FACS-analyses, Chris Oschatz for the skin vascular leakage assay, Evi Reichert for taking care of my mice, and Elfi Walter for always being around to help as a good fairy.    

My parents for their advice, encouragement, and financial support.  

... and especially my wife Katrin and my daughter Sarah for their support, patience, and never- ending love. You are my light if it is dark and my rock in stormy times.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

To Katrin and Sarah