Ever increasing demand for fossil fuels is a major factor for rapid depletion of these non-renewable energy resources, which has enhanced the interest of finding out alternative sources of energy. In recent years jatropha seed oil has been used extensively for production of bio-diesel and has shown significant potential to replace petroleum fuels at least partially. De-oiled jatropha seed cake (DOJSC) which comprises of approximately 55 to 65% of the biomass is a byproduct of bio-diesel industry. DOJSC contains toxic components like phorbol esters which restricts its utilization as animal feed. Thus along with the enhancement of biodiesel production from jatropha, there is an associated problem of handling this toxic byproduct. Utilization of DOJSC as a feed stock for production of biochemicals may be an attractive solution to the problem. Pullulan is an industrially important polysaccharide with several potential applications in food, pharmaceuticals and cosmetic industries. However, the major bottleneck for commercial utilization of pullulan is its high cost. A cost effective process for pullulan production may be developed using DOJSC as sole nutrient source which will in turn also help in utilization of the byproduct of bio-diesel industry. Results In the present study, DOJSC has been used as a nutrient for production of pullulan, in place of conventional nutrients like yeast extract and peptone. Process optimization was done in shake flasks, and under optimized conditions (8% DOJSC, 15% dextrose, 28°C temperature, 200 rpm, 5% inoculum, 6.0 pH) 83.98 g/L pullulan was obtained. The process was further validated in a 5 L laboratory scale fermenter. Conclusion This is the first report of using DOJSC as nutrient for production of an exopolysaccharide. Successful use of DOJSC as nutrient will help in finding significant application of this toxic byproduct of biodiesel industry. This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production.
Deoiledjatropha seed cake pullulan production 1* 2 3* Anirban Roy Choudhury , Nishat Sharma and GS Prasad
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Abstract Background:Ever increasing demand for fossil fuels is a major factor for rapid depletion of these nonrenewable energy resources, which has enhanced the interest of finding out alternative sources of energy. In recent years jatropha seed oil has been used extensively for production of biodiesel and has shown significant potential to replace petroleum fuels at least partially. Deoiled jatropha seed cake (DOJSC) which comprises of approximately 55 to 65% of the biomass is a byproduct of biodiesel industry. DOJSC contains toxic components like phorbol esters which restricts its utilization as animal feed. Thus along with the enhancement of biodiesel production from jatropha, there is an associated problem of handling this toxic byproduct. Utilization of DOJSC as a feed stock for production of biochemicals may be an attractive solution to the problem. Pullulan is an industrially important polysaccharide with several potential applications in food, pharmaceuticals and cosmetic industries. However, the major bottleneck for commercial utilization of pullulan is its high cost. A cost effective process for pullulan production may be developed using DOJSC as sole nutrient source which will in turn also help in utilization of the byproduct of biodiesel industry. Results:In the present study, DOJSC has been used as a nutrient for production of pullulan, in place of conventional nutrients like yeast extract and peptone. Process optimization was done in shake flasks, and under optimized conditions (8% DOJSC, 15% dextrose, 28°C temperature, 200 rpm, 5% inoculum, 6.0 pH) 83.98 g/L pullulan was obtained. The process was further validated in a 5 L laboratory scale fermenter. Conclusion:This is the first report of using DOJSC as nutrient for production of an exopolysaccharide. Successful use of DOJSC as nutrient will help in finding significant application of this toxic byproduct of biodiesel industry. This in turn also have a significant impact on cost reduction and may lead to development of a cost effective green technology for pullulan production. Keywords:Jatropha, Value addition to waste,Aureobasidium pullulans, Fermentation, Exopolysaccharide, Pullulan
Background Fossil fuels especially, fuels and commodities obtained from petroleum derived liquids play an important part in almost every aspect of our modern life. However, over exploitation of these natural resources to maintain modern amenities has caused negative ramifications on environmental as well as economical aspects of our life. Due to the limited nature of fossil fuels, their prices are
* Correspondence: anirban@imtech.res.in; prasad@imtech.res.in 1 Biochemical Engineering Research & Process Development Centre (BERPDC), CSIRInstitute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector 39A, Chandigarh 160 036, India 3 Microbial Type Culture Collection and Gene Bank (MTCC), CSIRInstitute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research (CSIR), Sector 39A, Chandigarh 160 036, India Full list of author information is available at the end of the article
expected to increase more rapidly in the near future [1]. Therefore, to reduce the use of fossil fuels there is a compelling need to search alternative sources of energy. Production of fuels and chemicals from renewable bio mass is becoming increasingly attractive and will be essential for our future and sustainability [2]. In recent years,Jatropha curcasL. has gained considerable atten tion as a potential source of biodiesel and many planta tions of jatropha have been established in tropical and subtropical regions worldwide [3,4]. SWOT (Strength, Weakness, Opportunity and Threat) analysis carried out on the feasibility of jatropha biofuels suggests that to make it economically viable it is important to utilize by products produced during the biodiesel production from jatropha. Deoiled jatropha seed cake (DOJSC) produced