Development of a miniaturized immunochemical flow-injection system for on-site analysis of selected nitroaromatics and pesticides in water [Elektronische Ressource] / Ioan Manuel Ciumasu

Grasunii

Informations

• Publié par: technische_universitat_munchen
• Publié le: 01 janvier 2006
• Nombre de lectures: 48
• Langue: English
• Poids de l'ouvrage: 1 Mo

Extrait

Technische Universität München
Wissenschaftszentrum Weihenstephan
Lehrstuhl für Ökologische Chemie und Umweltanalytik



Development of a miniaturized immunochemical flow-injection system for
on-site analysis of selected nitroaromatics and pesticides in water


Ioan Manuel Ciumasu


Vollständiger Abdruck der von der Fakultät Wissenschaftszentrum Weihenstephan für
Ernährung, Landnutzung und Umwelt der Technischen Universität München zur
Erlangung des akademischen Grades eines

Doktors der Naturwissenschaften (Dr. rer. nat.)

genehmigten Dissertation.



Vorsitzender: Univ.-Prof. Dr.rer.nat.habil. Wilfried Huber
Prüfer der Dissertation: 1. Univ.-Prof. Dr.rer.nat. Dr.h.c.(RO) Antonius Kettrup
2. Univ.-Prof. Dr.med.vet. Dr.med.vet.habil. Johann Bauer
3. Priv.-Doz. Dr.rer.nat. Petra Krämer


Die Dissertation wurde am 29.07.2005 bei der Technischen Universität München
eingereicht und durch die Fakultät Wissenschaftszentrum Weihenstephan für
Ernährung, Landnutzung und Umwelt am 09.11.2005 angenommen.



for my wife


Publications related to the PhD thesis
Related research articles:
1. Ioan M. Ciumasu, Petra M. Krämer, Cristina M. Weber, Günther Kolb, David Tiemann,
Stefan Windisch, Ines Frese, Antonius A. Kettrup. A new, versatile field immunosensor
for environmental pollutants. Development and proof of principle with TNT, diuron and
atrazine. Biosensors and Bioelectronics 21: 354-364.
http://www.sciencedirect.com/science/journal/9565663

2. Petra M. Krämer, Elisabeth Kremmer, Cristina M. Weber, Ioan M. Ciumasu, Stephan
Forster, Antonius A. Kettrup. Development of new rat monoclonal antibodies with
different selectivities and sensitivities for 2,4,6-trinitrotoluene (TNT) and other
nitroaromatic compounds. Analytical and Bioanalytical Chemistry 382: 1919-1933.
http://www.springerlink.com/(cams0xblxzdzdfjaeuhfquca55)/app/home/isue.asp

Related oral presentations:

3. I. Frese (oral presenter), G.Kolb, D. Tiemann, P.M. Krämer, I.M. Ciumasu, C.M. Weber,
2003. Development of an Optical Cell for an Automated Miniaturized Immunochemical
Device for On-site Screening of Pesticides Residues in Water. In: Proceedings of the
Oral presentation at "Sensor 2003" International Trade Fair and Conference, 13-15 May
2003, Nuremberg, Germany. Further information on the conference at :
http://www.sensorfairs.de/neu/SO3/index.html

4. G. Kolb (oral presenter), I. Frese, V. Hessel, I.M. Ciumasu, P.M. Krämer, H. Löwe, D.
Tiemann, 2004. An automated, portable immunochemical flow-injection system for on-
site analysis of environmentally hazardous chemicals. In: Proceedings of the Oral
Presentation at Lab Automation 2004, 1-5 February 2004, San Jose, CA, USA. Further
information on the conference at: http://labautomation.org/LA/LA04/conference

5. P.M. Krämer (oral presenter), I.M. Ciumasu, C.-M. Weber, A.A. Kettrup, D. Tiemann, I.
Frese, G. Kolb, 2004. Entwicklung eines neuen, tragbaren, automatisierten Einweg-
immunosensors für eine Vor-Ort-Auswahl von mit Umweltschadstoffen belasteten
Proben. Abschlussveranstaltung des Graduiertenkollegs 'Analytische Chemie', 2.–4.
Februar 2004, Blaubeuren.




Publications related to the PhD thesis
Related posters:

6. P.M. Krämer, I.M. Ciumasu, C.M. Weber, G. Kolb, I. Frese, B. Werner, A.A. Kettrup.
Development of an automated miniaturized immunochemical device for on-site
thscreening of pesticide residues in water. Poster at "The 10 IUPAC International
Congress on the Chemistry of Crop Protection", 4-9 August, 2002, Basel, Switzerland.
Congress proceedings, Main Topic 6 – Residues and Consumer Safety, Subtopic 6a –
Trends in Analytical Methods and Instrumentation, p 201;

7. I.M. Ciumasu, P.M. Krämer, C.M. Weber, G. Kolb, D. Tiemann, S. Windisch, I.Frese,
A.A. Kettrup. Single-use immunosensor for environmental pollutants. Proof of principle
for nitroaromatics and pesticide. The Eighth World Congress on Biosensors, 24-26 May
2004, Granada, Spain. Poster abstract (BS67) in the Congress Proceedings, Topic
"Immunosensors".

8. P.M. Krämer, C.-M. Weber, I.M. Ciumasu, E. Kremmer, A.A. Kettrup. Development of
monoclonal antibodies for 2,4,6-trinitrotoluene and its metabolites 2-amino-4,6-
dinitrotoluene and 4-amino-2,6-dinitrotoluene for their use in immunosensor. The Eighth
World Congress on Biosensors, 24-26 may 2004, Granada, Spain. Poster abstract
(BS632) in the Congress Proceedings, Topic "Immunosensors".

ACKNOWLEDGEMENTS

This work was funded by the Bmbf (Ministry for Science and Education, Germany),
project number FKZ 02WU0102. Bmbf also funded the work of the Institut für Mikrotechnik
Mainz GmbH (IMM), the instrument manufacturer, project number FKZ 02WU0102.

The author feels grateful for the PhD studentship and the technical support provided
by TUM, especially the Faculty of Nutrition, Agriculture and Environment, Department of
Ecological Chemistry and Environmental Analysis as PhD student all along the research
project (2001 – 2004) and for the finalization of the PhD thesis.
The author thanks GSF–National Research Centre for Environment and Health,
Neuherberg, Germany, especially the Institute of Ecological Chemistry, for hosting this work
and for insuring excellent research conditions during the research project.

The author thanks to Univ. Professor Dr. Antonius A. Kettrup for effective leadership
and kind supervision of this PhD work.

The author thanks to the project leader, Dr. Petra M. Krämer, for fruitful and pleasant
collaboration, and for precious help in forging a professional personality.

The author thanks Dr. Ing. Günther Kolb, Dipl.-Ing. David Tiemann and Dr. Ines
Frese, from IMM (Institut für Mikrotechnik Mainz), for their close collaboration.
The author thanks Dr. Elisabeth Kremmer, from GSF – Institute for Molecular
Immunology, for her collaboration during our research project.
The author thanks Dipl.-Chem. Hartmut Thomas from WASAG DECON GmbH,
BioPlanta, Leipzig for amiable exchange of scientific info and experience.

The author expresses his gratitude for the friendly collaboration of all the technical
and scientific staff from the GSF Institute for Ecological Chemistry, especially Dr. Sigurd
Schulte-Hostede – provisional director of the institute, Mrs Cristina Mihaela Weber, Dr.
Mariana Neamtu, Dr. Monica Pantiru, Dipl.-Ing. Annette Franke, Mr. Geza Cocsis, Ms.
Sylvia Oberleitner, Ms. Sabine Röttmuller and Mr. Stephan Forster.

But most of all I thank my wife, Maria Cristina Antal, for being so lovely all these
years of geographical quasi-separation.

Abbreviations and acronyms

Ab Antibody / antibodies
AFM Atomic force microscopy
Ag Antigen / antigens
An Analyte
ARIS Apoenzyme reactivation immunoassay
BSA Bovine serum albumine
CE Capillary electrophoresis
CEC electro-chromatography
CFI Continuous flow immunosensor
CR Cross-reactivity
CV Cyclic voltametry
DCC 1,3-Dicyclohexyl carbodiimide
Dil. Dilution
DL Detection limit
DMF Dimethyl formamide
DNP-glycine N-(2,4-dinitrophenyl)-glycine
DNP- γ-AmBA 2,4-Dinitrophenyl- γ-aminobutyric acid
DNP- ε-AmCA ε-aminocaproic acid
DNT Dinitrotoluene
DOM Dissolved organic matter
ECD Electron capture detection
EDC 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide
ELISA Enzyme-linked immunosorbent assay
EPA Environmental protection agency (USA)
Eq. Equation no
Fab Fraction antigen binding (of the Ab molecule)
Fc Fraction constant (of the Ab molecule)
FIA Flow-injection analysis
Fig. Figure no
FP Field prototype (of the developed immunosensor)
FRET Fluorescence resonance energy transfer
Gam Goat anti-mouse IgG
GC Gas chromatography
GSF GSF – National Research Centre for Environment and
Health, Neuherberg, Germany


Abbreviations and acronyms
HRMS High resolution mass spectrometry
HPLC High performance liquid chromatography
HRP Horseradish peroxidase (E.C. 1.11.1.7)
IgG Immunoglobuline
IC Ion chromatography
IC Analyte concentration producing 20% inhibition 20
IC oducing 50 50
ICP Inductively coupled plasma
IDLIF Indirect laser-induced fluorescence
Inc. Incubation
Ip Isoproturon
IMM Institut für Mikrotechnik Mainz
IÖC Institut für Ökologische Chemie - GSF
ITMS Ion trap mass spectrometry
k Association constant a
k Dissociation d
KLH Keyhole limpet hemocynine
LASER Light amplification by stimulated emission of radiation
LC Liquid chromatography
LP Laboratory prototype (of the developed immunosensor)
µ-TAS Micro-total-analysis-systems
mAb Monoclonal antibody
MECK Micellar