DNA block copolymers [Elektronische Ressource] : synthesis, morphologies and applications / Fikri E. Alemdaroglu

Alemdaro

Sujets

• Gesundheit

Informations

• Publié par: johannes_gutenberg-universitat_mainz
• Publié le: 01 janvier 2007
• Nombre de lectures: 19
• Langue: English
• Poids de l'ouvrage: 16 Mo

Extrait

DNA Block Copolymers –
Synthesis, Morphologies and Applications
Dissertation
zur Erlangung des Grades
“Doktor der Naturwissenchaften”
am Fachbereich
Chemie, Pharmazie und Geowissenschaften
der Johannes Gutenberg-Universität Mainz
Fikri E. Alemdaroglu
geb. in Adana
Mainz 2007 Dedicated to my wife, Ceren…Hayatta en hakiki mür it ilimdir!
(The real guide in life is science!)
M. Kemal ATATÜRK, 1923
Ya amayı ciddiye alacaksın
Yani o derece, öylesine ki,
Kocaman gözlüklerin,
Beyaz gömle inle bir laboratuarda insanlar için ölebileceksin.
Hem de yüzünü bile görmedi in insanlar için,
Hem de hiç kimse seni buna zorlamamı ken,
Hem de en güzel, en gerçek eyin ya amak oldu unu bildi in halde...
Nazım H KMET, 1947
??????????TABLE OF CONTENTS
Chapter 1…… DNA Meets Synthetic Polymers – Highly Versatile Hybrid
Materials “a literature review”…….………………………………… 15
Chapter 2…….Motivation and Objective….. ………………………………………. 39
Chapter 3…… Synthesis of ss DNA Block Copolymers………………………......... 45
Chapter 4…… Synthesis of DNA Multiblock Copolymers by Hybridization………. 69
Chapter 5…….Generation of Multiblock Copolymers by PCR: Synthesis,
Visualization and Nanomechanical Properties…………………........ 79
Chapter 6…… Dendritic Nanopatterns from DNA Diblock Copolymers…………... 97
Chapter 7…… Engineering the Structural Properties of DNA Block
Copolymer Micelles............................................................................. 109
Chapter 8…… Controlling the Size of Nanoparticles by an Enzymatic Reaction....... 127
Chapter 9…… DNA-templated Synthesis in Three Dimensions:
Introducing a Micellar Scaffold for Organic Reactions........................ 141
Chapter 10...... “Shape Matters”: Cellular Uptake of DNA Block Copolymer
Micelles.................................................................................................. 161
Chapter 11...... DNA Block Copolymer Micelles – A Combinatorial
Tool for Cancer Nanotechnology............................................................ 173
Chapter 12...... Summary.................................................................................................. 197
Curriculum vitae………………………………………………………... 203
Acknowledgements……………………………………………………... 207List of Abbreviations
A adenine
AFM atomic force microscopy
ASO antisense oligonucleotide
bp base pair
Caco-2 human colon adenocarcinoma cell line
C cytosine
CCD charge coupled device
CLSM confocal laser scanning microscopy
CMC critical micelle concentration
DBC DNA block copolymer
DCC N,N -dicyclohexylcarbodiimide
DIPEA diisopropyl ethylamine
DLS dynamic light scattering
DMEM Dulbecco’s modified Eagle’s medium
DMF N,N-dimethylformamide
DMSO dimethylsulfoxide
DMT-MM 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride)
DNA deoxyribonucleic acid
Dox Doxorubicin
DSMZ Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH
DTS DNA templated synthesis
dNTP deoxynucleotide triphosphate
ds double stranded
dTTP deoxythymidine triphosphate
EDC N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide
FA folic acid
FACS fluorescence-activated cell-sorting
FBS fetal bovine serum
FCS fluorescence correlation spectroscopy
FR Folate receptor
G guanine
?GPC gel permeation chromatography
HBSS Hank’s balanced salt solution
HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)
HOPG highly oriented pyrolytic graphite
HPLC high pressure liquid chromatography
LCST lower critical solution temperature
MALDI-TOF MS matrix assisted laser desorption / ionization – time of flight mass
spectrometry
MES 2-(N-morpholino)ethanesulfonic acid
M number average molecular weight n
M weight average molecular weight w
MW molecular weight
MWCO molecular weight cut-off
MOPS 3-(N-morpholino)propanesulfonic acid
NHS N-hydroxysuccinimide
NIPAM N-isopropylacrylamide
NMR nuclear magnetic resonance
OD optical density
ODN oligodeoxynucleotide
PAGE polyacrylamide gel electrophoresis
PCR polymerase chain reaction
PDI polydispersity index
PEG poly(ethylene glycol)
PEI polyethyleneimine
PEO poly(ethylene oxide)
PLGA poly(D,L-lactic-co-glycolic acid)
PLL poly(L-lysine)
PNIPAM poly(N-isopropylacrylamide)
PPO poly(propylene oxide)
PS polystyrene
RFC reduced folate carrier
RNA ribonucleic acid
ROMP ring-opening metathesis polymerization