Effect of Escherichia coli Stx1 on the cytokine profile of bovine ileal intraepithelial lymphocytes [Elektronische Ressource] / Etienne Moussay

Sujets

• Biologie

Informations

• Publié par: justus-liebig-universitat_giessen
• Publié le: 01 janvier 2006
• Nombre de lectures: 8
• Langue: English
• Poids de l'ouvrage: 1 Mo

Extrait

AusdemInstitutfürHygieneundInfektionskrankheitenderTiere
JustusLiebigUniversitätGießen






EffectofEscherichiacoli Stx1onthecytokineprofileof
bovineilealintraepitheliallymphocytes




Dissertation
zurErlangungdesDoktorgrades
derNaturwissenschaften
derJustusLiebigUniversitätGießen

FachbereichBiologie,ChemieundGeowissenschaften




EtienneMoussay
Gießen2006





Deanfheacultyfbiology: Prof.r...chreiner


st
1 eporter: Prof.r...artin

nd
2 eporter: Prof.r.r.abil.aljer




thDatefheralxamination: May9 006






















Myparents












“Decide&arefully,xactlywhatyouwantnife,henorkikeadoake ureyougett!”
Hector rawford


Publicationsandposters

Part of the work presented in this doctoral thesis was submit ted for publication under the
followingeference:

Moussaytienne ,tamm%vonne,aubert'nja,aljer*eorg,nd hristian#enge
Escherichia coli Shiga toxin 1 enhances il4 transcripts in bovine ileal intraepithelial
lymphocytes.
Submitted.

Andaspresented,sposters,t
heollowing&ongresses:

Etienne#oussay,vonnetamm,eorgaljer,hristian#enge
Shiga toxin 1 from E. coli induces an increase in IL4 mRNA synthesis in bovine ileal
thintraepithelial lymphocytes. Poster presented at the 56 Annual meeting of the DGHM
(GermanSocietyforHygieneandMicrobiology)inMünster(G ermany)@Abstractpublished
in the International Journal of Medical Microbiology, Vol. 294S1, Supplem ent N°39, Sept
2004,p23224.

Etienne#oussay,vonnetamm,eorgaljer,hristian#enge
Shiga toxin 1 from E. coli induces an increase in IL4 mRNA synthesis in bovine ileal
intraepitheliallymphocytes.PosterpresentedattheannualCR WAD(ConferenceofResearch
Workersnnimal iseases)nt.Louis>Missouri,SA),005.








Abreviations

7AAD 7aminoctinomycin
Abs Absorbance
ActD Actinomycin
A/E Attaching/effacing
AP1 Activationprotein1
APC Antigenpresenting&ell
ATCC Americanypeculturecollection
A.U. Arbitrarynits
BCR Bcelleceptor
BCV Bovine&oronavirus
BL3 Bovine#ymphoma3
BLV Bovine#eukemiairus
bp basepair
BSA Bovineerumlbumine
CD Clusterfifferentiation
CD Cytotoxicosisor0%of
he&ells50
ConA Concanavalin
DCs Dendritic&ells
DEPCwater Diethylpyrocarbonate
reatedater
DiO Dioctadecyloxacarbocyaninperchlorate
DN Doubleegative>CD4 *D8 #ymphocytes?
DNA Desoxiribonucleiccid
+ +DP Doublepositive>CD4 *D8 #ymphocytes)
DTT 1.4ithiotreitol
EBV EpsteinBarrirus
ECACC Europeancollectionfnimal&ell&ultures
E.oli Escherichiaoli
EDTA Ethylenediamineetraaceticcid
EF12) Elongationactor1(2)
EHEC EnterohemoragicEscherichiaoli
EPEC EnteropathogenicEscherichiaoli

ER Endoplasmiceticulum
Esp Escherichiaoli ecretedprotein
FACS Fluorescentactivatedellorting
FAE Follicleassociatedpithelium
f.c. Final&oncentration
FCS Fetal&alferum
FITC Fluoresceinsothiocyanat
GALT Gutssociatedymphoidissue
Gb /CD77 Globotrioacylceramid3
γ Commonchainromnterleukineeceptorsc
GMCSF Granulocyteandonocytecolonytimulatingactor
hrs Hours
HIV Humanmmunodecifiencyvirus
HUS Haemolyticremicyndrome
IEC Intestinalepithelial&ell
IEL Intraepithelial#ymphocyte
iIEL Ilealntraepithelial#ymphocyte
IFNγ Interferongamma
Ig Immunoglobulinrmmunoglobin
IL Interleukin
ILF Isolatedymphoidollicle
IP10 Interferoninducedpeptide0
Kb Kilobase
kDa Kilodalton
KGF Keratinocytegrowthacotr
LEE Locusfnterocyteeffacement
LFA Leukocyte