Functional thin films fabricated by plasma polymerization and their biological applications [Elektronische Ressource] / Li-Qiang Chu

Chu

Informations

• Publié par: johannes_gutenberg-universitat_mainz
• Publié le: 01 janvier 2007
• Nombre de lectures: 19
• Langue: English
• Poids de l'ouvrage: 2 Mo

Extrait

Functional Thin Films Fabricated by Plasma Polymerization
and Their Biological Applications



Dissertation
zur Erlangung des Grades
„Doktor der Naturwissenschaften“



am Fachbereich Chemie, Pharmazie und Geowissenschaften
der Johannes Gutenberg-Universität Mainz




Li-Qiang Chu
geb. in Liaoning, V. R. China


Mainz, 2007

Tag der mündlichen Prüfung: 09 May, 2007






















































……To my wife and my parents










Contents - i -

CONTENTS
CONTENTS ............................................................................................................................. I
ABBREVIATIONS............................................................................................................... IV
1. GENERAL INTRODUCTION ..........................................................................................1
1.1 BACKGROUND ................................................................................................................1
1.2 AIMS OF THE PRESENT STUDY .........................................................................................3
REFERENCES.........................................................................................................................4
2. THEORY..............................................................................................................................7
2.1 PLASMA POLYMERIZATION .............................................................................................7
2.1.1 Plasma and plasma reactors..................................................................................7
2.1.2 Plasma polymerization .........................................................................................7
2.2 SURFACE PLASMON OPTICS.............................................................................................9
2.2.1 Surface plasmon resonance...................................................................................9
2.2.2 SPR with prism coupling....................................................................................11
2.2.3 Optical waveguide spectroscopy ........................................................................14
2.2.4 Surface plasmon enhanced fluorescence spectroscopy ......................................17
REFERENCES.......................................................................................................................19
3. EXPERIMENTAL SECTION..........................................................................................21
3.1 MATERIALS AND SUBSTRATES ......................................................................................21
3.2 PLASMA POLYMERIZATION ...........................................................................................22
3.3 SPR AND OWS MEASUREMENTS..................................................................................24
3.4 SPFS MEASUREMENTS..................................................................................................25
3.5 OTHER CHARACTERIZATION TECHNIQUES.....................................................................27
REFERENCES.......................................................................................................................28
4. NON-FOULING COATINGS BY PLASMA POLYMERIZATION...........................29
4.1 INTRODUCTION .............................................................................................................29
4.2 EXPERIMENTAL SECTION ..............................................................................................30
4.2.1 Preparation of pp-EO2 films...............................................................................30
4.2.2 Protein adsorption measured by SPR .................................................................30
4.3 RESULTS AND DISCUSSION............................................................................................31
4.3.1 Film analysis.......................................................................................................31
4.3.2 Protein adsorption measured by SPR .................................................................35
4.3.3 Discussion...........................................................................................................39
4.4 CONCLUSIONS...............................................................................................................40
- i -- ii - Contents

REFERENCES ...................................................................................................................... 40
5. PROTEIN-RESISTANT DNA SENSOR........................................................................ 43
5.1 INTRODUCTION ............................................................................................................ 43
5.2 EXPERIMENTAL SECTION.............................................................................................. 44
5.2.1 Preparation of ppEO2 coatings .......................................................................... 44
5.2.2 Preparation of DNA sensor................................................................................ 44
5.2.3 Activity of the embedded streptavidin ............................................................... 45
5.2.4 DNA hybridization monitored by SPFS ............................................................ 45
5.3 RESULTS AND DISCUSSION ........................................................................................... 46
5.3.1 Optimization of separation layer........................................................................ 46
5.3.2 Streptavidin immobilization by extremely thin ppEO2 ..................................... 48
5.3.3 Effect of streptavidin concentration................................................................... 50
5.3.4 Optimization of ppEO2 cover layer ................................................................... 51
5.3.5 DNA detection from DNA/protein mixture solution ......................................... 55
5.3.6 Regeneration of DNA sensors............................................................................ 59
5.3.7 Limit of detection............................................................................................... 60
5.4 CONCLUSIONS.............................................................................................................. 61
REFERENCES ...................................................................................................................... 61
6. PLASMA POLYMERIZED EPOXIDE COATING FOR DNA SENSOR ................. 63
6.1 INTRODUCTION ............................................................................................................ 63
6.2 EXPERIMENTAL SECTION.............................................................................................. 64
6.2.1 Preparation of ppGMA films ............................................................................. 64
6.2.2 Immobilization of NH -DNA............................................................................. 64 2
6.2.3 DNA hybridization measured by SPFS ............................................................. 65
6.3 RESULTS AND DISCUSSION ........................................................................................... 65
6.3.1 Film analysis ...................................................................................................... 65
6.3.2 DNA probe immobilization ............................................................................... 67
6.3.3 DNA hybridization............................................................................................. 68
6.4 CONCLUSIONS.............................................................................................................. 71
REFERENCES ...................................................................................................................... 71
7. STABILIZATION OF ALLYLAMINE PLASMA FILMS.......................................... 73
7.1 INTRODUCTION ............................................................................................................ 73
7.2 EXPERIMENTAL SECTION.............................................................................................. 74
7.2.1 Deposition of ppAA films.................................................................................. 74
7.2.2 Ethanol extraction of ppAA films...................................................................... 74
7.2.3 SPR and OWS measurements............................................................................ 74
7.3 RESULTS AND DISCUSSION .............