Identification and characterization of phospholipase A_1tn2 from Trypanosoma brucei [Elektronische Ressource] = Identifizierung und Charakterisierung von Phospholipase A_1tn2 aus Trypanosoma brucei / vorgelegt von Khalid Muhammad

Khalid Muhammad

Sujets

• Biologie
• Gesundheit

Informations

• Publié par: eberhard_karls_universitat_tubingen
• Publié le: 01 janvier 2009
• Nombre de lectures: 101
• Langue: English
• Poids de l'ouvrage: 3 Mo

Extrait

Identification and characterization of phospholipase A 2
from Trypanosoma brucei

Identifizierung und Charakterisierung von Phospholipase A 2
aus Trypanosoma brucei







DISSERTATION




der Fakultät für Chemie und Pharmazie
der Eberhard Karls Universität Tübingen


zur Erlangung des Grades eines Doktors
der Naturwissenschaften

2009

vorgelegt von

Khalid Muhammad






































Tag der mündlichen Prüfung: 15. September 2009

Dekan: Prof. Dr. Lars Wesemann

1. Berichterstatter: Prof. Dr. Michael Duszenko

2. Berichterstatter: Prof. Dr. Klaus Schulz-Osthoff























I dedicate this thesis to all human kind



























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Acknowledgement


I express my deepest appreciation and gratitude to Prof. Dr. Michael Duszenko for his sincere
guidance, encouragement and generous supervision through out this research project and
providing me the opportunity to work on this project.
I am thankful and grateful to my scholarship DAAD (German academic exchange service) for
giving me the opportunity to come to Germany not only to learn the culture, language but also
perform, experience and complete my scientific research.
I am very much indebted with gratitude to Dr. Syed Abid Ali for his indefinite support,
advices and in particular appreciate his assistance in my research. I would like to thank Dr.
Hubert Kalbacher and the lab members of his group for synthesizing peptide and providing
me the antibody.
I would use this opportunity to thank the people who helped me during this thesis, my entire
research work and specifically in everyday life and making my stay in Germany memorable.
Firstly, I express my heartily thanks and gratitude to Dr. Patrick Merkel for his friendship,
moral and scientific help with productive suggestions during my stay in lab. I like to express
my outmost thanks to Dr. Viola Denninger for her friendly discussions, moral as well as
scientific support and night walks back home when we missed our last bus. Next, I am very
much indebted to Rudolf Koopman for his friendliness, assistance in my thesis, our common
project discussions, and friendly advices. I express my gratitude to Bjoern Bassarak for his
cooperation and help during this project and productive scientific discussions. I deeply
appreciate Caroline Schoenfeld for her outgoing friendly assistance and technical assistance
during my research work. I would like to thank to Bjoern Buchholz (my lab table neighbor)
for his friendly over the table discussions, exchange of jokes and assisting me with the
German translations and technical problems. I thank Torsten Barth for keeping the
environment lively with his friendly attitude. Stefan Mogk for giving me the company in late
night/weekend lab sessions and Gordon Eske for his all out supports in every aspect and he is
a true gentleman. I would like to thank Witta Kaiser for her friendly nature and assistance
during this work. Derya Demirsal (my other lab table neighbor) for her assistance, friendliness
during the work which brought encouragement. Andrea Wegner, for her friendliness and help
in the lab.
Paula Quecke, my intern for her friendly assistance and helping me out in this project. I am
grateful to Felix Klug for his friendly and productive talks.
I I

My dear cousin and friend, Saad Aman (May God bless his soul) whom I can not forget, his
smiling face, caring friendship and for good memories. My friend I owe you a lot.

I am really grateful and want to show heartily, warmest thanks to my dear friends from the
bottom of my heart for providing me home-like environment and friendly company in my
difficult times during my stay in Germany. Obrigado, Gabriel Da Silva for his brotherly
friendship, patient listening to my problems and supporting me in every way. Grazie, Elena
Ritossi for her lovely cooking, friendship and lively attitude. Tesekkür for Yonca Ural, a good
friend, her caring attitude, friendship, affectionate nature, for motivations and above all her
nice discussions especially over Biochemistry.
Last but not at least, I am really thankful, Buhat buhat shukrya to my dear family for
encouraging and assisting me during my whole studies and giving me motivation in
accomplishing this work. I would like to thank my parents, my father Dr. Hanif Muhammad
Shaikh and mother Surriya Begum for their care, love and support during whole of my life
and studies which I can not repay in any way and during my life time. I like to give my
deepest thank to my elder brother Tariq Muhammad Shaikh for his moral support, advices and
assistance in every way. My deepest and heartily thank to my dearest and loving sister Abeer
Khan for her love, care and her friendship.
Finally, I like to apologize to all those people and friends I have forgotten to mention here
who made contributions to not only towards this thesis and research but played a role in my
life.

















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Abbreviations


AA Arachidonic acid
AP Alkaline phosphatase
APS Ammonium persulfate
BCIP 5-Bromo-4-chloro-3-indolyl phosphate
BCS Bathocuproine disulfonic acid
BF Bloodstream form
bp Base pair
BSA Bovine serum albumin
CGA Citrate glucose anticoagulant
CNS Central nervous system
COX Cycloxygenase
DEAE Diethylaminoethyl
DG Diacylglycerol
DL-PC Dilinolenoyl phosphatidylcholine
DMSO Dimethylsulfoxide
DMG Dimyristoylglycerol
DTT Dithiothreitol
EC Enzyme commission
EDTA Ethylenediaminetetraacetic acid
ELISA Enzyme-linked immunosorbent assay
FBS Fetal bovine serum
FFA Free fatty acid
GPCho Glycerophosphocholines
GPI Glycosylphosphatidylinositol
HEPES N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid
HETE Hydroxyeicosatetraenoic acid
IPTG Isopropyl-beta-D-thiogalactopyranoside
kDa kilo dalton
LO Lipoxygenase
LT Leukotrienes
MEM Minimum essential medium
MITat Molteno institute trypanozoon antigenic type
I V