Influence of peroxisomes on development, maturation and adult functions of the testis [Elektronische Ressource] / Anca Nenicu

Nenicu, Anca

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• Publié par: justus-liebig-universitat_giessen
• Publié le: 01 janvier 2010
• Nombre de lectures: 4
• Langue: English
• Poids de l'ouvrage: 13 Mo

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Influence of peroxisomes on development,
maturation and adult functions of the testis
ANCA NENICU
INAUGURAL DISSERTATION
submitted to the Faculty of Medicine
in fulfillment of the requirements
for the PhD-degree of the
édition scientifique Faculties of Veterinary Medicine and Medicine
VVB LAUFERSWEILER VERLAG of the Justus Liebig University Giessen
VVB LAUFERSWEILER VERLAG
STAUFENBERGRING 15 ISBN: 978-3-8359-5617-9
D-35396 GIESSEN
Tel: [phone] Fax: -5599890
[e-mail]
www.doktorverlag.de 9 7 8 3 8 3 5 9 5 6 1 7 9
édition scientifique
VVB LAUFERSWEILER VERLAGVVB
ANCA NENICU PEROXISOME IN TESTICULAR CELLSDas Werk ist in allen seinen Teilen urheberrechtlich geschützt.
Jede Verwertung ist ohne schriftliche Zustimmung des Autors
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1. Auflage 2010
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st1 Edition 2010
© 2010 by VVB LAUFERSWEILER VERLAG, Giessen
Printed in Germany
édition scientifique
VVB LAUFERSWEILER VERLAG
STAUFENBERGRING 15, D-35396 GIESSEN
Tel: [phone] Fax: [phone]
email: [e-mail]
www.doktorverlag.de
Institute for Anatomy and Cell Biology II
Faculty of Medicine of the Justus Liebig University Giessen







Influence of peroxisomes on development,
maturation and adult functions of the testis







Inaugural Dissertation
submitted to the
Faculty of Medicine
in fulfillment of the requirements
for the PhD-degree
of the Faculties of Veterinary Medicine and Medicine
of the Justus Liebig University Giessen








Anca Nenicu


Giessen 2010

From the Institute for Anatomy and Cell Biology II
of the Faculty of Medicine of the Justus Liebig University of Giessen
Director / Chairperson: Prof. Dr. Eveline Baumgart-Vogt


















First Supervisor and Committee Member: Prof. Dr. Eveline Baumgart-Vogt

Second Supervisor and Committee Member: Priv.-Doz. Dr. Joachim Weitzel

Committee Members: Prof. Dr. Martin Diener

Priv.- Doz. Dr. Lutz Konrad













Date of Doctoral Defense: 20th August 2010
























My parents


























“If you doubt you can accomplish something, then you can't accomplish it. You have to have
confidence in your ability, and then be tough enough to follow through.”
Rosalyn Carter







Declaration


“I declare that I have completed this dissertation single-handedly without the unauthorized
help of a second party and only with the assistance acknowledged therein. I have
appropriately acknowledged and referenced all text passages that are derived literally from
or are based on the content of published or unpublished work of others, and all information
that relates to verbal communications. I have abided by the principles of good scientific
conduct laid down in the charter of the Justus Liebig University of Giessen in carrying out the
investigations described in the dissertation.”


thGiessen, April 30 2010 Anca Nenicu


































List of Abbreviations


3 β-HSD 3 -hydroxysteroid dehydrogenase
17β-HSD 17 
17OH-P 17-hydroxypregnenolone
17OH-Pre 17-hydroxyprogesterone
ABC ATP-binding cassette family of transporters
ACOX Acyl-CoA oxidase
AMH Anti-Müllerian hormone
APS Ammonium persulfate
BSA Bovine serum albumin
CAT Catalase
cDNA Complementary deoxyribonucleic acid
COX Cyclooxygenase
CYP450arom Cytochrome P450 aromatase
CYP450scc Cytochrome P450 side-chain cleavage
°C Degree celcius
4-A androstenedione
5-A androstanediol
DHEA Dehydroepiandrosterone
DHT Dihydrotestosterone
DMSO Dimethyl sulfoxide
DNA Deoxyribonucleic acid
DTT 1,4 ‐dithio‐DL ‐threitol
EDTA Ethylene‐diamine tetraacetate
ER Endoplasmic reticulum
FSH Follicle-stimulating hormone
GFP Green fluorescent protein
h Hour(s)
HSD Hydroxysteroid dehydrogenase
HTZ Heterozygote
IF Immunofluorescence
IHC Immunohistochemistry
IL Interleukin
KH PO Potassium dihydrogen phosphate 2 4
KO Knockout
LCFA Long-chain fatty acid
LH Luteinizing hormone
min Minute(s)
M Molar
MFP-2 Multifunctional protein-2
mg Milligram
ml Millilitre
Na HPO Disodium hydrogen phosphate 2 4
NaOH Sodium hydroxide
ng Nanograms
% Percentage
PBD Peroxisome biogenesis disorder
PBS Phosphate ‐buffered saline
PBST ‐buffered saline with Tween
PCR Polymerase chain reaction
Pex Gene encoding a peroxin (peroxisome biogenesis protein) PGE Prostaglandin
PFA Paraformaldehyde
PMP Peroxisomal membrane protein
PPAR Peroxisome proliferator activated receptors
PTS targeting signal
PUFA Polyunsaturated fatty acids
RNA Ribonucleic acid
ROS Reactive oxygen species
RT Room temperature
RXR Retinoic X receptor
SDS‐PAGE Sodium dodecyl sulfate polyacrylamide gel electrophoresis
s Second(s)
sER Smooth endoplasmic reticulum
SF-1 Steroidogenic factor 1
siRNA Small interfering RNA
SOD Superoxide dismutase
StAR Steroidogenic acute regulator protein
T Testosterone
TAE Tris acetate EDTA buffer
TEMED N, N, N, N ‐tetramethylethylenediamine
THIOLASE peroxisome 3-ketoacyl-CoA thiolase
Tris Tris (hydroxymethyl) aminomethane
μg Micrograms
μl Microliter
μm Micrometer
VLCFA Very long-chain fatty acid
v/v Volume/volume
WB Western blot
WT Wild-type
w/v Weight/volume
X-ALD X-linked Adrenoleukodystrophy
ZS Zellweger syndrome



Literature overview
1. Literature overview .......................................................................................................... 4
1.1. Overview on the male reproductive system ................................................................ 4
1.1.1. Structure of the adult testis.................................................................................. 4
1.1.2. Development of the testis.................................................................................... 4
1.1.3. The interstitial cells – Leydig cells ....................................................................... 5
1.1.3.1. Leydig cells - Target for hormones and mediator of hormone effects.............. 6
1.1.3.2. Growth factors – regulation of Leydig cells ..................................................... 6
1.1.3.3. Production of steroid hormones in Leydig cells............................................... 8
1.1.4. The testicular seminiferous tubule - structure and function................................ 11
1.1.4.1. The Peritubular myoid cells .......................................................................... 11
1.1.4.2. The Sertoli cell.............................................................................................. 11
I. Structure ................................................................................................................ 11
II. Maintenance of the integrity of the seminiferous epithelium ............................... 12
III. Functions of Sertoli cells ................................................................................... 13
1.1.5. Spermatogenesis .............................................................................................. 17
1.2. Peroxisomes............................................................................................................. 19
1.2.1. Nomenclature and morphology of peroxisomes ................................................ 19
1.2.2. Biogenesis of peroxisomes ............................................................................... 20
1.2.2.1. Peroxisomal matrix protein import and its receptors ........................