Investigation of mammary blood flow changes by transrectal colour Doppler sonography in an Escherichia coli mastitis model [Elektronische Ressource] / by André Potapow
From the Clinic for Ruminants with Ambulatory and Herd Health Services (Chair for Physiology and Pathology of Reproduction) (Head: Professor Doctor Holm Zerbe) Centre of Clinical Veterinary Medicine of the Ludwig-Maximilians-University Munich Investigation of mammary blood flow changes by transrectal colour Doppler sonography in an Escherichia coli mastitis model Inaugural-Dissertation to achieve the Doctor Title of Veterinary Medicine at the Faculty of Veterinary Medicine of the Ludwig-Maximilians-University Munich by André Potapow from Amstetten Munich, April 2010 Gedruckt mit der Genehmigung der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München Dekan: Univ.-Prof. Dr. Braun Berichterstatter: Univ.-Prof. Dr. Zerbe Korreferent/en: Univ.-Prof. Dr. Poulsen Nautrup Tag der Promotion: 24. Juli 2010 51. II To my parents III FELIX QUI POTUIT RERUM COGNOSCERE CAUSAS PUBLIUS VERGILIUS MARO (GEORGICA. II, 490 et sq.) IVIndex ofcontets Page 1. Introduction 1 2. Physiology of the mammary blood flow in cows 3 2.1 Vascularization of the mammary gland 2.2 Regulation of mammary blood flow 4 3. Bovine mastitis 9 3.1 Definition and classification 9 3.
From the Clinic for Ruminants with Ambulatory and Herd Health Services (Chair for Physiology and Pathology of Reproduction) (Head: Professor Doctor Holm Zerbe) Centre of Clinical Veterinary Medicine of the Ludwig-Maximilians-University Munich Investigation of mammary blood flow changes by transrectal colour Doppler sonography in anEscherichia colimastitis model Inaugural-Dissertation to achieve the Doctor Title of Veterinary Medicine at the Faculty of Veterinary Medicine of the Ludwig-Maximilians-University Munich by André Potapow from Amstetten Munich, April 2010
Univ.-Prof. Dr. Braun
Univ.-Prof. Dr. Zerbe
Univ.-Prof. Dr. Poulsen Nautrup
Gedruckt mit der Genehmigung der Tierärztlichen Fakultät der Ludwig-Maximilians-Universität München Dekan: Berichterstatter: Korreferent/en:
Tag der Promotion: 24. Juli 2010
II
51.
To my parents
I
III
FELIX QUI POTUIT RERUM COGNOSCERE CAUSAS
IV
PUBLIUS VERGILIUS MARO (GEORGICA. II, 490 et sq.)
Introduction
Physiology of the mammary blood flow in cows Vascularization of the mammary gland Regulation of mammary blood flow
Bovine mastitis Definition and classification Escherichia colimastitis
Mammary blood flow measurements by Doppler sonography Basic principles of Doppler sonography Instrumental equipment Methods of analysis
Investigation of mammary blood flow changes by transrectal colour Doppler sonography in anEscherichia colimastitis model Discussion Establishment of a new non-invasive method for monitoring mammary blood flow in cowsTCDS a method for early mastitis detection? Future prospects
Summary
Zusammenfassung
References
Acknowledgements
V
1
3 3 4
9 9 10
13 13 15 16
18
26 26 30 36
37
39
41
57
Index of abbreviations [ ] a.i. B mode -BE [BFV] [c] CFU CMT [D] EC E. coli[f] [fo] GH IGF [Min] MY p.i. PGE PGF2[PI] PMN p.p. [RI] ROS RT [S] SCC [TAMF] TCDS [v]
Doppler-angle (°) after infusion brightness modulation bacteriological examination blood flow volume (l/min) velocity of sound in tissue (1540 m/s) colony-forming unit California Mastitis Test end-diastolic frequency shift (Hz) electrical conductivity Escherichia coli Doppler-shift frequency (Hz)transmitted frequency (Hz) growth hormone insulin-like growth factor minimal diastolic frequency shift (Hz) milk yield post infectionem prostaglandin E prostaglandin F2 Pulsatility Index polymorphonuclear neutrophilic leukocytes post partum Resistance Index reactive oxygen species rectal temperature maximal systolic frequency shift (Hz) somatic cell count time averaged maximum frequency shift (Hz) transrectal colour Doppler sonography blood flow velocity (m/s)