Nanofibers and nanoparticles by electrostatic processing for medical and pharmaceutical applications [Elektronische Ressource] / vorgelegt von Dirk Grafahrend

Dirk Grafahrend

Sujets

• Gesundheit

Informations

• Publié par: rheinisch-westfalischen_technischen_hochschule_-rwth-_aachen
• Publié le: 01 janvier 2009
• Nombre de lectures: 35
• Langue: English
• Poids de l'ouvrage: 12 Mo

Extrait

“Nanofibers and nanoparticles by electrostatic processing for
medical and pharmaceutical applications”


Von der Fakultät für Mathematik, Informatik und Naturwissenschaften der RWTH Aachen
University zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften
genehmigte Dissertation


vorgelegt von


Diplom-Chemiker
Dirk Grafahrend
aus Aachen

Berichter: Universitätsprofessor Dr. rer. nat. Martin Möller
Professorin Dr. rer. nat. Doris Klee

Tag der mündlichen Prüfung: 14. Dezember 2009

Diese Dissertation ist auf den Internetseiten der Hochschulbibliothek online
verfügbar.








Mich erstaunen die Leute, die das Universum begreifen wollen, wo es schwierig genug ist,
in Chinatown zurechtzukommen.



Woody Allen, 01.12.1935
Drehbuchautor, Schauspieler und Regisseur











Je planmäßiger die Menschen vorgehen, desto wirksamer vermag sie der Zufall zu treffen.



Friedrich Dürrenmatt, 05.01.1921 – 14.12.1990
Schriftsteller, Dramatiker und Graphiker

- Acknowledgements -
Acknowledgements

The practical work forming the basis of this PhD thesis was mainly carried out at the Institute
of Technical and Macromolecular Chemistry (ITMC) of Aachen University of Technology
(RWTH) and the German Wool Research Institute (DWI) at the RWTH Aachen under
guidance of Professor Dr. Martin Moeller and Professor Dr. Doris Klee during the period
from July 01, 2004 to July 30, 2008. I thank both, Professor Dr. Martin Moeller and Professor
Dr. Doris Klee for the brilliant guidance, the interesting subject, and the freedom while
completing this thesis as well as in choosing cooperation network.
I would like to thank especially those coworkers without whom this thesis would not have
been possible. Special thanks to Dr. Paul D. Dalton and Kristina Klinkhammer for the melt-
electrospinning of PCL in chapter 3, Ibrahim Hassounah for help with melt-electrospinning of
polypropylene in chapter 3, Martin Rosenthal for the WAXD and SAXD experiments in
chapter 4, Robert Loesel for the support (synthesis and electrospinning experiments during his
diploma thesis) in chapter 7, and Karl-Heinz Heffels, Peter Gasteier and Dr. Jürgen Groll for
sharing the experimental work and planning of chapter 8, and last but not least I would like to
thank Tino Schreiber for his practical and scientific support. Professor Dr. Dimitri A. Ivanov
is acknowledged for providing the opportunity to accomplish the practical work regarding the
XRD and SAXS investigations at the Beamline 26 at ISRF facilities in Grenoble. Here, I
would like to thank the following people without whom this work would not have been
possible:
I gratefully acknowledge valuable advice and support during the everyday laboratory life and
the permanent disposition for discussion especially provided by Dr. Paul D. Dalton, Dr.
Jürgen Groll, Dr. Helmut Keul, Dr. ―Sacha‖ Xiaomin Zhu, Dr. Helga Thomas, Dr. Jochen
Salber, Dr Barbara Dittrich, Mr. Peter Gasteier, Mrs. Birgit Penkert, Mrs. Petra Ampen, Mrs.
Júlia Lleixà Calvet, Mrs. Kristina Klinkhammer, Mr. Robert Lösel, Mr. Ibrahim Hassounah,
Mr. Tino Schreiber, Mr. Holger Leonards, Mr. Marc Hans, Mrs. Nicole Fricke, Mr. Christof
Schmitz, Mrs Wiebke Voigt, Mr. Ralf Krüll, Mr. Jörg Heinrichs, Mr. Karl-Heinz Heffels,
Mrs. Alexandra Goldyn and Mrs. Angela Plum. All co-workers of the ITMC and the German
Wool Research Institute (DWI) at the RWTH Aachen are acknowledged for their
cooperativeness and for providing a comfortable working atmosphere and perfect working
conditions.
4 - Acknowledgements -
I would like to acknowledge the DFG Graduierten Kolleg 1035 ―Biointerface‖ and like to
thank all participants for the interesting insights into the various different topics as well as the
interdisciplinary team work. Prof. Dr. Thomas Kissel and Dr. Erik Rytting - for supervising
the chapter on charged polymer and drug release, giving me the chance to participate at the
GRC on Drug-Delivery and editing the manuscript, Dr. Dimitri A. Ivanov and Mr. Martin
Rosenthal- for writing the simulation and interpretation of the x-ray scattering (WAXS and
SAXS), Professor Dr. Claus-Micahel Lehr, Dr. Michael Bur and Mr. Andreas ―Andi‖
Henning - for the Discussions and work clearance mechanisms after pulmonary deposition,
Dr. Michael Krüger and Dr. Regis Cartier of Boehringer Ingelheim Pharma GmbH & Co.KG
for the interesting discussions, the insights into pharmaceutical industry and the coordination
of the ―NanoInhale‖ project, Dr. Thomas Schmehl and Dr. Thomas Gaessler - for the fruitful
discussions, Dr. Helga Thomas - for the good communication and discussions on
electrospinning. I would like to acknowledge all the cooperation partners, especially from the
NanoInhale‖ project whom I forgot mention. I would like to thank all other PhD students
involved in these projects for the nice and friendly atmosphere.
I am grateful for the financial support of the research presented in this thesis to the Deutsche
Forschungsgemeinschaft for the DFG Graduierten Kolleg 1035 ―Biointerface‖, the
Bundesministerium für Bildung und Forschung for the BMBF project 13N8888 „NanoInhale
- Nano-Carrier mit zeitlich und örtlich gesteuerter Freisetzung von Wirkstoffen zur
Inhalation―, the Arbeitsgemeinschaft industrieller Forschungsvereinigungen "Otto von
Guericke " e.V. for the two projects AIF 14263N „Nanofaservliese für die Therapie von
Oberflächenwunden―, and the AIF 14324N „Electrospinning aus wässriger Lösung und aus
der Schmelze zur Herstellung antimikrobiell wirksamer Nanofasern mit hohem Ad- und
Absorptionsvermögen für den Einsatz in Filtermaterialien―.
I am very thankful to all my friends, especially Martha Rygula, Andreas Bürger, Alexander
Hack, Pascal Schäfer, Sven Englert, Michi und Markus Willenberg, Holger Weist, Thorsten
Hoffmann, Reyes Lopez Garcia, and all the others, who supported me in my private life.
Thank you very much! Special thanks to Paul ―Mono‖ Steinbusch for the coffee and endless
talks on life in general while the DWI was still located in the old building. And I am also very
grateful to my family for their enormous support.
Without the support of all of you this work would have been hardly possible.
I per acabar, voldria agrair a la meva companya Júlia Lleixà Calvet, amb qui comparteixo el
dia a dia, la seva estima, confiança i suport.
Aachen, Januar 2009
5 - Publications -

6 - Publications -
List of Publications

Parts of this thesis are published, submitted to be published or presented at conferences.


Articles:

1. D. Grafahrend, K.-H. Heffels, M. Moeller, D. Klee, and J. Groll, Electrospun,
Biofunctionalized Fibers as Tailored In-VitroSubstrates for Keratinocyte Cell Culture,
Macromolecular Bioscience, DOI: 10.1002/mabi.201000068, 2010.
2. K. Klinkhammer, J. Bockelmann, C. Simitzis, G.A. Brook, D. Grafahrend, J. Groll, M.
Möller, J. Mey, D. Klee, Functionalization of electrspun fibers of poly(ε-caprolactone) with
star shaped NCO-poly(ethylene glycol)-stat-poly(propylene glycol) for neuronal cell guidance
J. Mater. Sci.: Mater. Med , DOI: 10.1007/s10856-010-4112-7, 2010
3. R. Loesel, D. Grafahrend, M. Moeller, D. Klee, Bioresorable Electrospun Fibers for
Immobilization of Thiol Containing Compounds, Macromolecular Bioscience, DOI:
10.1002/mabi.201000101, 2010.
4. D. Grafahrend, P. Jungbecker, G. Seidel, H. Leonards, T. Gries, M. Moller, D. Klee,
Development and Optimization of an Electrospraying Device for the Continuous Collection of
Nano- and Microparticles, The Open Chemical and Biomedical Methods Journal, 2010, 1-
9.
5. Henning, M. Schneider, N. Nafee, E. Rytting, X. Wang, T. Kissel, D. Grafahrend, D.
Klee, C.-M. Lehr, Influence of Particle Size and Surface Properties on Mucociliary
Clearance from the Airways, Journal of Aerosol Medicine and Pulmonary Drug Delivery,
2010, DOI: 10.1089/jamp.2009.0806, 2010.
6. R. Lösel, D. Furlani, N. Paul, D. Grafahrend, K. Hemmrich, N. Ma, M. Möller, D.
Klee, Biofunctionalized Polymer Nonwovens as Scaffolds for Soft Tissue Engineering,
BIOmaterialien, 2009, (10), 88.
7. R. Lösel, D. Grafahrend, M. Möller, D. Klee, Functionalized Electrospun Fiber
Structures as Scaffolds for Endothelial Cell Growth, BIOmaterialien, 2008, 9, 129.
8. K.-H. Heffels, P. Gasteier, D. Grafahrend, J. Salber, M. Möller, J. Groll, An easy route
to biofunctional electrospun fibres with reactive star shaped PEO-stat-PPO based molecules
for tissue engineering, BIOmaterialien, 2008, 9, 100.
7 - Publications -
9. K. Klinkhammer, N. Seiler, D. Grafahrend, J. Gerardo-Nava, J. Mey, G. A. Brook, M.
Möller, P. D. Dalton, D. Klee, Deposition of electrospun fibers on reactive substrates for in
vitro investigations, Tissue Engineering Part C, 2009, 15(1), 77-85.
10. D. Grafahrend, J. Lleixa Calvet, J. Salber, P. D. Dalton, D. Klee, M. Möller, Co