Neurochemical and functional characterisation of the melanin concentrating hormone system in the rat brain [Elektronische Ressource] / von Thomas Appl

Thomas Appl

Sujets

• Biologie

Informations

• Publié par: universitat_potsdam
• Publié le: 01 janvier 2007
• Nombre de lectures: 17
• Langue: English
• Poids de l'ouvrage: 13 Mo

Extrait

Universität Potsdam/
Deutsches Institut für Ernährungsforschung (DIfE)
Abteilung Molekulare Genetik
Bergholz-Rehbrücke, Deutschland


Boehringer-Ingelheim Pharma GmbH & Co KG
Abteilung Stoffwechselforschung
Biberach/Riss, Deutschland





Neurochemical and functional
characterisation of the
melanin-concentrating hormone (MCH)
system in the rat brain



Dissertation zur Erlangung des akademischen Grades
"doctor rerum naturalium"
(Dr. rer. nat.)
in der Wissenschaftsdisziplin "Neurobiologie"

eingereicht an der Mathematisch-Naturwissenschaftlichen Fakultät
der Universität Potsdam



von
Thomas Appl






















































Elektronisch veröffentlicht auf dem
Publikationsserver der Universität Potsdam:
http://opus.kobv.de/ubp/volltexte/2007/1460/
urn:nbn:de:kobv:517-opus-14604
[http://nbn-resolving.de/urn:nbn:de:kobv:517-opus-14604] General abbreviations:
ACC Acetyl-Coenzyme A MCHR-1 melanin concentrating hormone receptor subtype 1
ACC2 Acetyl-Coenzyme A carboxylase type 2 MCHR-2 melanin concentrating hormone receptor subtype 2
ACRP-30 adipocyte complement related protein-30 MCH (-/-) MCH deficient mice
ACTH adrenocorticotropic hormone MCHR-1 (-/-) MCH deficient mice
aCSF artificial cerebrospinal fluid MGOP MCH-gene-overprinted-polypeptide
AgRP Agouti related peptide min minute
AIDS aquired immunodeficiency syndrome ml milliliter
AL ad libitum fed animals mm millimeter
AN Anorexi nervosa µm micrometer
ANP Atrio-natriuretic peptide MRI Magnet resonance imaging
AROM antisense-RNA-overlapping-MCH-gene mRNA messenger ribonucleic acid
BAT brown adipose tissue α-MSH alpha- melanocyte stimulating hormone
BF brightfield NaCl sodium chloride
BIA Bioelectrical Impedance analysis NDS normal donkey serum BMI Body mass index Neo/Neo neonatal MCH deficient mice
BMR Basal metabolic rate NEAT non-exercise activity thermogenesis BSA bovine serum albumine NEI neuropeptide-glutaminc acid isoleucine
BW body weight NGE neuropeptide glycine-glutamic acid cAMP cyclo-adenosin monophosphate NPY neuropeptide Y
CART cocaine- and amphetamine regulated transcript Ob-R Leptin receptor
CBR-1 Cannabinoid receptor subtype-1 Ob-Rb Leptin receptor subtype
CCK Cholecystokinin ob/ob leptin-deficient mice
CD C Center for disease control and prevention OT,OXY oxytocin
cDNA complementary desoxyribonucleic acid OxA Orexin A
CHO chinese ovary hamster p.o. per os (oral)
CNS central nervous system PBS phosphate buffer saline
CNTF ciliary neurotrophic factor PET Positron emission tomography
CRH Corticotropin-realsing hormone PFA paraformaldehyde
CT Computed Tomography PGC-1α peroxisome proliferator-activated receptor γ
DAPI 4`,6-diamidino-2-phenylindole dilactate pmch pro MCH gene
db/db leptin receptor deficient mice POMC pro-opiomelanocortin
DGAT diacylglycerol acetyltransferase PP Pancreatic polypeptide
DIO diet-induced obese PPARγ peroxisome proliferator-activated receptor γ
FAS fatty acid synthase Co-activator I α
FD food deprived animals PPH primary pulmonary hypertension FEO food entrained oscillator ppMCH prepro Melanin-concentrating hormone
FR food restricted animals PTP-1B protein-tyrosine phosphatase-1B g gram PVDF Polyvinylidenfluorid
GnRH Gonadotropin releasing hormone PYY(3-36) Peptide YY (Fragment 3-36) GLP Glucagon like peptide RFS restricted feeding schedules
GPCR G-protein coupled receptor ROI region of interest h hour RT room temperature
HDL high density lipids s.c. subcutaneous HPLC high performance liqid chromatography
SDS-PAGE sodium dodecyl sulfate –
11-ß HSD 11-ß hydroxysteroid-dehydrogenase polyacrylamide gel electrophoresis
5-H T 5-Hydroxytryptamine (serotonin) sec second
5-HT 5-Hydroxytryptamine receptor subtype 2c 2c SNP single nucleotide polymorphism
HRP horse radish peroxidase SST1-5 somatostatin receptor subtypes 1-5
i.c. intracerebral TBS-T Tris buffered solution with Triton X-100
i.c.v. intracerebroventricular TRH Thyrotropin-releasing hormone
i.e. id est TRITC tetramethylrhodamine isothiocyanate
IEG immediate early gene TSA tyramide signal amplification
IgG,IgY Immunoglobulin G, Immunoglobulin Y TSH thyreodea-stimulating hormone
i.n. intranuclear US Ultra Sound
i.p. intraperitoneal USA United States of America
IF immunofluorescence VP Vasopressin IR immunoreactive, immunoreactivity w/v weight/volume
Kcal kilocalorie WHO World health organisation kg kilogram WHR Waist-to-hip circumference ratio
LHRH luteinizing hormone releasing hormone Y1,2,5-R Neuropeptide Y receptor subtype 1,2,5 M molar ZT Zeitgeber time
MAP mitogen activated protein
MC3,4 Melanocortin receptor subtypes 3,4 2
MCH melanin concentrating hormone
mch1-r MCHR-1 gene

Abbreviation of brain structures:

3V third ventricle MD mediodorsal thalamic nuclei
4V fourth ventricle ME median eminence
A amygdala MHb medial habenular nucleus
AAV anteroventral thalamic nucleus MM medial mammillary nucleus
aca anterior commisure MPO medial preoptic area
ACB nucleus accumbens NAc Nucleus accumbens
AcbC Accumbens nucleus (core) NLL nucleus of the lateral lemniscus
AcbSh Accumbens nucleus (shell) NTS,Sol nucleus of the solitary tract
AD anterodorsal thalamic nucleus 3 occulomotor nucleus
AHA anterior hypothalamic area OPT,oc,
AI Agranular insula cortex ocb olivary complex
AON anterior olfactory nucleus OT olfactory tubercle
APT anterior pretectal nucleus ox optic chiasm
Arc/ARC arcuate hypothalamic nucleus Par Parietal cortex
BLA Basolateral amydaloid nuclei PB parabrachial nuclei
BNST bed nucleus of the stria terminalis Pe periventricular hypothalamic nucleus
CA1 CA 1 region of the hippocampus PeF perifornical nucleus
CA2 CA 2 region of the hippocampus PF parafascicular thalamic nucleus
CA3 CA 3 region of the hippocampus PFA perifornical area
Cbgr granular cell layer of the cerebellar cortex PH posterior hypothalamic area
Ce Central amygdaloid nuclei Pir Piriform Cortex
CG periaqueductal grey Pn pontine reticular nucleus
CG/RSA, Pr5 principle sensory trigeminal nucleus
RSG cingulate retrosplenial cortex PSV principle sensory trigeminus nerve
CIC inferior colliculus PT paratenial thalamic nucleus
CL centrolateral thalamic nucleus PV paraventricular thalamic nucleus CM entralmedial thalamic nucleus PVN paraventricular hypothalamic nuclei
GP Globus pallidus PVP posterior paraventricular thalamic nucleus CPu Caudate Putamen R Red nucleus
CTX cortex Re reuniens thalamic nucleus
D3V dorsal third ventricle RMg, DR Raphe nuclei
DEn Dorsal endopiriform nucleus RT reticular thalamic nucleus
DG Dentate Gyrus S Subiculum
DMH dorsomedial hypothalamus SC superior collicus
DpM e dorsal part of the medial amygdaloid nucleus SCh Suprachiasmatic nucleus
DTg dorsal tegmental area SI substantia innominata
ec external capsule SNR substantia nigra (pars reticulata)
f fornix SNC substantia nigra (pars compacta)
FR frontal cortex SO suprapotic nucleus
GI granular insular cortex Sol nucleus of the solitary tract
HDB nucleus horizontal limb diagonal band Sp Spinal trigeminal nucleus
HDB nucleus of the horizontal limb of the diagonal band SPV spinal tract of the trigeminus nerve
Hi hippocampus STh subthalamic nucleus
IAM interanterodorsal thalamic nuclei SUB subiculum
IC inferior colliculus SuM Supramammillary nucleus
Icj Islets of Calleja TM, TMN tuberomammillary nucleus
IP Interpeduncular nuclei TT Tenia Tecta III oculomotor nerve VE vestibular nucleus
IMD intermediodorsal thalamic nucleus VL ventrolateral thalamic nucleus
Int interpositus cerebellar nucleus VLG,DLG geniculate nuclei (ventrolateral/dorsolateral
LC locus coerulus VM ventromedial thalamic nuclei
LH, LHA lateral hypothalamus VMH ventromedial hypothalamic nuclei
LHB, lateral habenular nucleus VMP ventral poster