Recombinant IgG expression in mammalian cells [Elektronische Ressource] / von Jiandong Li

Jiandong Li

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• Gesundheit

Informations

• Publié par: technische_universitat_carolo-wilhelmina_zu_braunschweig
• Publié le: 01 janvier 2006
• Nombre de lectures: 26
• Langue: Deutsch
• Poids de l'ouvrage: 1 Mo

Extrait

Recombinant IgG expression in mammalian cells



Von der Fakultät für Lebenswissenschaften
der Technischen Universität Carolo-Wilhelmina
zu Braunschweig













zur Erlangung des Grades
eines Doktors der Naturwissenschaften
(Dr. rer. nat.)






genehmigte
D i s s e r t a t i o n






von Jiandong Li
aus Hebei, VR China














































1. Referent: Prof. Dr. Stefan Dübel
2. Referent: Prof. Dr. Jürgen Bode
eingereicht am: 04.05.2006
mündliche Prüfung (Disputation) am: 26.07.2006
Druckjahr: 2006


iiName: Jiandong Li
Anschrift: Emsstr. 2a, 38120 Braunschweig

Ich erkläre hiermit an Eides Statt, dass ich

1. die vorliegende Dissertation mit dem Thema

selbständig verfasst,
noch nicht veröffentlicht
nicht als Diplomarbeit oder ähnliche prüfungsarbeit verwendet
und die benutzten Hilfsmittel vollständig angegeben,

2. früher ein Promotionsgesuch noch nicht eingereicht habe


Datum:...........................................................


........................................................................
(Unterschrift)


iiiIm Zusammenhang mit der Thematik der vorliegenden Dissertation wurden bzw. werden
folgende Publikationen erstellt:



Publications (in process):
Jiandong Li, Christian Menzel, Doris Meier, Congcong Zhang, Stefan Dübel, Thomas Jostock. A
comparative study of different vector designs for the mammalian expression of recombinant IgG
antibodies.

Jiandong Li, Congcong Zhang, Christian Menzel, Stefan Dübel, Thomas Jostock. The impact of
the relative ratio of heavy and light chain expression on IgG production in mammalian cells.

Posters presented on international congress:
Jiandong Li, Doris Meier, Stefan Dübel and Thomas Jostock. (2005) A comparative study of
different vector designs for mammalian expression of recombinant IgG antibodies. 4th
International Congress on Recombinant Antibodies, Hilton Berlin, Germany.

Lars Toleikis, Jiandong Li, Doris Meier, Thomas Jostock and Stefan Dübel. (2005) A human
antibody for targeting adenocarcinomas. 4th International Congress on Recombinant Antibodies,
Hilton Berlin, Germany.
ivIndex



Content






Recombinant IgG expression in mammalian cells ········································································· i
1 Summary···································································································································· 1
2 Introduction·· 3
2.1 Immunoglobulins·············································································································· 3
2.2 Recombinant antibodies··································································································· 4
2.3 IgG expression in mammalian cells ················································································· 5
2.4 Eukaryotic gene expression····························································································· 6
2.5 Protein translation in eukaryotic cells··············································································· 8
2.6 Internal ribosomal entry site (IRES) mediated cap-independent translation ···················· 9
2.7 EMCV IRES ··················································································································· 10
2.8 Scaffolds/matrix attached region (S/MAR) ····································································· 12
2.9 Recombinase mediated cassette exchange (RMCE) ···················································· 13
2.10 Breast Cancer ·············································································································· 15
2.11 Mucin 1 (MUC1)·············