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Taurine reduces the secretion of apolipoprotein B100 and lipids in HepG2 cells

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Higher concentrations of serum lipids and apolipoprotein B100 (apoB) are major individual risk factors of atherosclerosis and coronary heart disease. Therefore ameliorative effects of food components against the diseases are being paid attention in the affluent countries. The present study was undertaken to investigate the effect of taurine on apoB secretion and lipid metabolism in human liver model HepG2 cells. Results The results demonstrated that an addition of taurine to the culture media reduces triacylglycerol (TG)-mass in the cells and the medium. Similarly, cellular cholesterol-mass was decreased. Taurine inhibited the incorporation of [ 14 C] oleate into cellular and medium TG, suggesting the inhibition of TG synthesis. In addition, taurine reduced the synthesis of cellular cholesterol ester and its secretion, suggesting the inhibition of acyl-coenzyme A:cholesterol acyltransferase activity. Furthermore, taurine reduced the secretion of apoB, which is a major protein component of very low-density lipoprotein. Conclusion This is a first report to demonstrate that taurine inhibits the secretion of apoB from HepG2 cells.
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Lipids in Health and Disease
BioMedCentral
Open Access Research Taurine reduces the secretion of apolipoprotein B100 and lipids in HepG2 cells 1 1,21 1 Teruyoshi Yanagita*, SeoYoung Han, Ying Hu, Koji Nagao, 3 3 Hideaki Kitajimaand Shigeru Murakami
1 2 Address: Departmentof Applied Biochemistry and Food Science, Saga University, Saga 8408502, Japan,Jeonnam Natural Resources Research 3 Institute, 756 Kisanri Anyangmyeon Jangheunggun Jeollanamdo 529851, Korea andR&D Headquarters, Self Medication Business, Taisho Pharmaceutical Co., Ltd., Tokyo 1708633, Japan Email: Teruyoshi Yanagita*  yanagitt@cc.sagau.ac.jp; SeoYoung Han  syoung@mail.goo.ne.jp; Ying Hu  yhu@cc.sagau.ac.jp; Koji Nagao  knagao@cc.sagau.ac.jp; Hideaki Kitajima  hkitajima@so.taisho.co.jp; Shigeru Murakami  smurakami@so.taisho.co.jp * Corresponding author
Published: 17 October 2008Received: 1 October 2008 Accepted: 17 October 2008 Lipids in Health and Disease2008,7:38 doi:10.1186/1476-511X-7-38 This article is available from: http://www.lipidworld.com/content/7/1/38 © 2008 Yanagita et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract Background:Higher concentrations of serum lipids and apolipoprotein B100 (apoB) are major individual risk factors of atherosclerosis and coronary heart disease. Therefore ameliorative effects of food components against the diseases are being paid attention in the affluent countries. The present study was undertaken to investigate the effect of taurine on apoB secretion and lipid metabolism in human liver model HepG2 cells. Results:The results demonstrated that an addition of taurine to the culture media reduces triacylglycerol (TG)-mass in the cells and the medium. Similarly, cellular cholesterol-mass was 14 decreased. Taurine inhibited the incorporation of [C] oleate into cellular and medium TG, suggesting the inhibition of TG synthesis. In addition, taurine reduced the synthesis of cellular cholesterol ester and its secretion, suggesting the inhibition of acyl-coenzyme A:cholesterol acyltransferase activity. Furthermore, taurine reduced the secretion of apoB, which is a major protein component of very low-density lipoprotein. Conclusion:This is a first report to demonstrate that taurine inhibits the secretion of apoB from HepG2 cells.
Background Taurine,βsulphonic amino acid was first isolated more than 150 years ago from ox bile [1]. Taurine is considered to be an essential nutrient for human infants and cats and distributed extensively in mammalian cells and tissues [1,2]. Its recognized metabolic function in liver is conju gation with bile acids, which is important for bile secre tion and lipid digestion [3]. Nevertheless, taurine also has beneficial effects on the liver that include prevention and
treatment of cholestasis and prevention of liver damaged due to toxic chemicals [46].
In past decades, much attention has been paid to the effects of taurine on bile acid metabolism. Conversely, a few studies concerning the role of taurine on the other liver lipids have been reported. Liver is the central organ of lipid metabolism. Previous in vitro studies with human hepatoblastoma cells showed that cellular levels of tau
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