The combination of high-fat diet-induced obesity and chronic ulcerative colitis reciprocally exacerbates adipose tissue and colon inflammation

Teixeira, De, Leonel, Aguilar, Batista, Alves, Coimbra, Ferreira, Cara, Alvarez

Description

This study evaluated the relationship between ulcerative colitis and obesity, which are both chronic diseases characterized by inflammation and increases in immune cells and pro-inflammatory cytokines. Methods Mice with chronic ulcerative colitis induced by 2 cycles of dextran sodium sulfate (DSS) in the first and fourth week of the experiment were fed a high-fat diet (HFD) to induce obesity by 8 weeks. The animals were divided into 4 \ groups (control, colitis, HFD and colitis + HFD). Results Obesity alone did not raise histopathology scores, but the combination of obesity and colitis worsened the scores in the colon compared to colitis group. Despite the reduction in weight gain, there was increased inflammatory infiltrate in both the colon and visceral adipose tissue of colitis + HFD mice due to increased infiltration of macrophages, neutrophils and lymphocytes. Intravital microscopy of VAT microvasculature showed an increase in leukocyte adhesion and rolling and overexpression of adhesion molecules compared to other groups. Moreover, circulating lymphocytes, monocytes and neutrophils in the spleen and cecal lymph nodes were increased in the colitis + HFD group. Conclusion Our results demonstrated the relationship between ulcerative colitis and obesity as aggravating factors for each disease, with increased inflammation in the colon and adipose tissue and systemic alterations observed in the spleen, lymph nodes and bloodstream.

Sujets

• Ulcerative colitis
• Obesity

Informations

• Publié par: biomed
• Publié le: 01 janvier 2011
• Nombre de lectures: 8
• Langue: English

Extrait

Teixeira et al . Lipids in Health and Disease 2011, 10 :204 http://www.lipidworld.com/content/10/1/204

R E S E A R C H Open Access The combination of high-fat diet-induced obesity and chronic ulcerative colitis reciprocally exacerbates adipose tissue and colon inflammation Lílian G Teixeira 1* , Alda J Leonel 1 , Edenil C Aguilar 1 , Nathália V Batista 2 , Andréa C Alves 1 , Candido C Coimbra 3 , Adaliene VM Ferreira 4 , Ana Maria C de Faria 1 , Denise C Cara 2 and Jacqueline I Alvarez Leite 1

Abstract Background: This study evaluated the relationship between ulcerative colitis and obesity, which are both chronic diseases characterized by inflammation and increases in immune cells and pro-inflammatory cytokines. Methods: Mice with chronic ulcerative colitis induced by 2 cycles of dextran sodium sulfate (DSS) in the first and fourth week of the experiment were fed a high-fat diet (HFD) to induce obesity by 8 weeks. The animals were divided into 4 \ groups (control, colitis, HFD and colitis + HFD). Results: Obesity alone did not raise histopathology scores, but the combination of obesity and colitis worsened the scores in the colon compared to colitis group. Despite the reduction in weight gain, there was increased inflammatory infiltrate in both the colon and visceral adipose tissue of colitis + HFD mice due to increased infiltration of macrophages, neutrophils and lymphocytes. Intravital microscopy of VAT microvasculature showed an increase in leukocyte adhesion and rolling and overexpression of adhesion molecules compared to other groups. Moreover, circulating lymphocytes, monocytes and neutrophils in the spleen and cecal lymph nodes were increased in the colitis + HFD group. Conclusion: Our results demonstrated the relationship between ulcerative colitis and obesity as aggravating factors for each disease, with increased inflammation in the colon and adipose tissue and systemic alterations observed in the spleen, lymph nodes and bloodstream. Keywords: ulcerative colitis, obesity, high-fat diet-induced obesity and inflammation

Background superficial lesions. In more severe cases, the entire colon Ulcerative colitis is a disease of incompletely understood  can be affected [1,2]. etiology and is characterized by inflammation of the The epidemiology of inflammatory bowel disease sug- colonic mucosa. In its chronic form, inflammation gests that environmental factors such as personal extends into the muscle layer of the colon, with acute hygiene, smoking and diet contribute to disease onset (symptomatic) manifestations, asymptomatic periods [3]. Pro-inflammatory markers including IL(interleukin)- and relapses after months, years or even decades. 6, IL-1 and TNF(tumor necrosis factor)-alpha are Ulcerative colitis inflammation affects the rectum and  increased in colitis. These markers are also increased in extends in a retrograde manner, with extensive areas of obesity that have an inflammatory component [4,5]. Obesity is a multifactorial d isease involving endocrine factors, genetics and behavior and directly contributes * Correspondence: [e-mail] hav ab- 1 Department of Biochemistry and Immunology - Institute of Biological to systemic inflammation. Several studies e est Sciences -Universidade Federal de Minas Gerais, Av. Antonio Carlos, 6627, lished a relationship between weight gain and levels of F P u a l m ll p is u t lh o a f , a B u e t l h o or H i o n ri f z o o r n m t a e t , io M n G is C a E v P: ail 3 a 1 b 2 l 7 e 0 a - t 90 t 1 h , e B e ra n z d il ofthearticle inflammatory proteins such as TNF, IL-1, IL-6 and © 2011 Teixeira et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.