Time-dependent modulation of pheromone-sensitive trichoid sensilla of the hawkmoth Manduca sexta [Elektronische Ressource] = Tageszeitabhängige Modulation von pherohormonsensitiven Trichoidsensillen des Tabakschwärmers Manduca sexta / vorgelegt von Christian Flecke

Christian Flecke

Sujets

• Biologie

Informations

• Publié par: philipps-universitat_marburg
• Publié le: 01 janvier 2009
• Nombre de lectures: 24
• Poids de l'ouvrage: 30 Mo

Extrait

Time-dependent modulation of pheromone-
sensitive trichoid sensilla of the hawkmoth
Manduca sexta


Tageszeitabhängige Modulation von pheromonsensitiven Trichoidsensillen
des Tabakschwärmers Manduca sexta














Dissertation zur Erlangung des
Doktorgrades der Naturwissenschaften
(Dr. rer. nat.)








dem Fachbereich Biologie
der Philipps-Universität Marburg
vorgelegt von
Christian Flecke
aus Schwalmstadt
Marburg/Lahn 2009




















































Time-dependent modulation of pheromone-sensitive
trichoid sensilla of the hawkmoth Manduca sexta

Tageszeitabhängige Modulation von pheromonsensitiven
Trichoidsensillen des Tabakschwärmers Manduca sexta



Dissertation zur Erlangung des
Doktorgrades der Naturwissenschaften
(Dr. rer. nat.)

















dem Fachbereich Biologie
der Philipps-Universität Marburg
vorgelegt von
Christian Flecke
aus Schwalmstadt
Marburg/Lahn 2009




Vom Fachbereich Biologie
der Philipps-Universität Marburg als Dissertation am 2008 angenommen.

Erstgutachter: Herr Prof. Dr. Uwe Homberg
Zweitgutachterin: Frau Prof. Dr. Monika Stengl


Tag der mündlichen Prüfung am 2009.












Diese Doktorarbeit widme ich allen,
die den Unterschied zwischen
Wissenschaft und Leidenschaft kennen.









For those who know the difference
between science and passion.





















































Contents
Contents

Erklärung: Eigene Beiträge und veröffentlichte Teile der Arbeit 1

Zusammenfassung 3
Referenzen 10

Introduction 13
The peripheral olfactory system of Manduca sexta ......................................................................... 13
Structure of pheromone-sensitive sensilla........................................................................................ 13
Insect olfactory transduction ............................................................................................................ 14
Role of cyclic nucleotides and biogenic amines in insect olfactory transduction ............................ 16
Circadian rhythms in insect olfaction............................................................................................... 17
Resolution of pulsed pheromone signals.......................................................................................... 19
References 20


Chapter I: Perfusion with cGMP analogue adapts the action potential
response of pheromone-sensitive sensilla trichoidea of the hawkmoth
Manduca sexta in a daytime-dependent manner 25

Introduction 25
Materials and methods 26
Animals and preparation for electrophysiological recordings.......................................................... 26
Application of 8bcGMP ................................................................................................................... 27
Pheromone stimulation..................................................................................................................... 27
Acquisition protocols and data analysis ........................................................................................... 27
Results 29
Pheromone responses ....................................................................................................................... 29
Effects on the amplitude reduction................................................................................................... 31
Spontaneous APs.............................................................................................................................. 33
Discussion 33
The role of cGMP in insect olfactory adaptation ............................................................................. 34
Daytime-dependent differences in the cGMP effects....................................................................... 37
References 38


Chapter II: Octopamine and tyramine modulate pheromone-sensitive
olfactory sensilla of the hawkmoth Manduca sexta in a time-dependent
manner 43

Introduction 43
Materials and methods 44
Application of OA, TA and EPI....................................................................................................... 45
Pheromone stimulation..................................................................................................................... 45
Acquisition protocols ....................................................................................................................... 45
Data analysis .................................................................................................................................... 46
Analysis of spontaneous APs and bursts.......................................................................................... 46

I Contents
Results 47
Effects on the kinetics of the AP response ....................................................................................... 49
Effects on the amplitude reduction................................................................................................... 50
Effects on the spontaneous APs ....................................................................................................... 51
Effects of epinastine ......................................................................................................................... 51
Discussion 51
OA and TA disadapt peripheral ORNs during the photophase ........................................................ 53
Time dependency of biogenic amine effects.................................................................................... 56
Effects on the AP distribution .......................................................................................................... 57
References 58


Chapter III: Perfusion with cAMP analogue affects pheromone-sensitive
trichoid sensilla of the hawkmoth Manduca sexta in a time-dependent
manner 63

Introduction 63
Material and methods 64
Animals and preparation .................................................................................................................. 64
Application of 8bcAMP and pheromone stimulation....................................................................... 64
Acquisition protocols ....................................................................................................................... 65
Data analysis .................................................................................................................................... 65
Analysis of spontaneous APs and bursts.......................................................................................... 65
Results 65
Perfusion with 8bcAMP increased the SP amplitude time- and BAL-dose-dependently ................ 66
Effects on the AP distribution in BAL responses............................................................................. 70
Effects on the spontaneous AP frequency........................................................................................ 70
Discussion 72
8bcAMP affects pheromone transduction but does not mimic all OA effects ................................. 72
SP amplitude increase without strong effects on the AP frequency................................................. 74
Effects on the spontaneous AP frequency. 75
Time-dependency of 8bcAMP effects.............................................................................................. 77
References 77


Curriculum vitae 81
Danksagung 85
Erklärung 87

II Erklärung
Erklärung: Eigene Beiträge und veröffentlichte Teile der Arbeit

Laut §8, Absatz 3 der Promotionsordnung der Philipps-Universität Marburg (Fassung vom 28.04.1993)
müssen bei den Teilen der Dissertation, welche aus gemeinsamer Forschungsarbeit entstanden sind, „die
individuellen Leistungen des Doktoranden deutlich abgrenzbar und bewertbar sein“. Dies betrifft die
Kapitel 1-3. Die Beiträge werden im Folgenden näher erl