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Principles of Gene Manipulation and Genomics

672 pages
The increasing integration between gene manipulation and genomics is embraced in this new book, Principles of Gene Manipulation and Genomics, which brings together for the first time the subjects covered by the best-selling books Principles of Gene Manipulation and Principles of Genome Analysis & Genomics.
  • Comprehensively revised, updated and rewritten to encompass within one volume, basic and advanced gene manipulation techniques, genome analysis, genomics, transcriptomics, proteomics and metabolomics
  • Includes two new chapters on the applications of genomics
  • An accompanying website - www.blackwellpublishing.com/primrose - provides instructional materials for both student and lecturer use, including multiple choice questions, related websites, and all the artwork in a downloadable format.
  • An essential reference for upper level undergraduate and graduate students of genetics, genomics, molecular biology and recombinant DNA technology.
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The first edition ofPrinciples of Gene Manipulationwas published over 25 years ago when the recombinant DNA era was in its infancy and the idea of sequenc-ing the entire human genome was inconceivable. In writing the first edition, the aim was to explain a new and rapidly growing technology. The basic philosophy was to present the principles of gene manipulation, and its associated techniques, in sufficient detail to enable the non-specialist reader to understand them. However, as the techniques became more sophisti-cated and advanced, so the book grew in size and complexity. Eventually, recombinant DNA techno-logy advanced to the stage where the sequencing and analysis of entire genomes became possible. This gave rise to a whole new biological discipline, known as genomics, with its own principles and associated techniques. From this emerged the first edition of another book,Principles of Genome Analysis, whose title changed toPrinciples of Genome Analysis and Genomicsin its third edition to reflect the rapid growth of post-sequencing technologies aiming at the large-scale analysis of gene function. It is now five years since the draft human genome sequence was published and we are reaching the stage where the technologies of gene manipulation and genomics are becoming increasingly integrated. Genome map-ping and sequencing technologies borrow exten-sively from the early recombinant DNA technologies of library construction, cloning, and amplification using the polymerase chain reaction; gene transfer to microbes, animals, and plants is now widely used for the functional analysis of genomes; and the applications of genomics and recombinant DNA are becoming difficult to separate. This new edition, entitledPrinciples of Gene Mani-pulation and Genomics, therefore unites the themes covered formerly by the two separate books and pro-vides for the first time a fully integrated approach to the principles and practice of gene manipulation in the context of the genomics era. As in previous editions of the two books, we have written the text at
an advanced undergraduate level, assuming a basic knowledge of molecular biology and genetics but no knowledge of recombinant DNA technology or genomics. However, we are aware that the book is favored not only by newcomers to the field but also by experts, and we have tried to remain faithful to both audiences with our coverage. As before we have not changed the level at which the book is written nor the general style, but we have divided the book into sections to enable the book to be used in different ways by different readers. The basic methodologies are presented in the first part of the book, which is devoted to cloning in Escherichia coli, while more advanced gene-transfer techniques (applying to other microbes and to ani-mals and plants) are presented in the second part. The reader who has read and understood the mate-rial in the first part, or already knows it, should have no difficulty in understanding any of the material in the second part of the book. The third part moves from the basic gene-manipulation technologies to genomics, transcriptomics, proteomics, and metabo-lomics, the major branches of the high-throughput, large-scale biology that has become synonymous with the new millennium. Finally, the fourth part of the book contains two chapters that discuss how recombinant DNA technology and genomics are being applied in the fields of medicine, agriculture, diagnostics, forensics, and biotechnology. In writing the first part of the book, we thought carefully about the inclusion of early “historical” information. Although older readers may feel that some of this material is dated, we elected to leave much of it in place because it has an important bear-ing on today’s methods and an understanding of it is incorrectly assumed in many of today’s publications. We have included such information where it illus-trates how modern techniques and procedures have evolved, but we have tried not to catalog outmoded or redundant methods that are no longer used. This is particularly the case in the genomics section
where new technologies seem to come and go every day, and few stand the test of time or become truly indispensable. We have aimed to avoid as much jargon as possible, and to explain it clearly where it is absolutely necessary. As is common in all areas of science, the principles of gene manipulation and genomics abound with acronyms and synonyms which are often confusing particularly now molecu-lar biology is becoming increasingly commercial in both basic research and its applications. Where appro-priate, we have provided lists of definitions as boxes set aside from the text. Boxes are also used to illustrate key experiments or principles, historical information,
and applications. While the text is fully referenced throughout, we have also provided a list of classic papers and reviews at the end of each chapter to ease the wary reader into the scientific literature. This book would not have been possible without the help and advice of many colleagues. Particular thanks are due to Sue Goddard and her library staff at HPA Porton for assistance with many literature searches. Sandy Primrose would like to dedicate this book to his wife Jill and Richard Twyman would like to dedicate this book to his parents, Irene and Peter, to his children Emily and Lucy, and to Liz for her end-less support and encouragement.