An electrophysiological analysis of synaptic transmission at the Drosophila larval neuromuscular junction [Elektronische Ressource] / vorgelegt von Daniel Bucher

Sujets

• Biologie

Informations

• Publié par: julius-maximilians-universitat_wurzburg
• Publié le: 01 janvier 2008
• Nombre de lectures: 24
• Langue: English
• Poids de l'ouvrage: 4 Mo

Extrait

An Electrophysiological Analysis of Synaptic Transmission
at the Drosophila Larval Neuromuscular Junction
Dissertation zur Erlangung des
naturwissenschaftlichen Doktorgrades
der Bayerischen Julius-Maximilians-Universität Würzburg
vorgelegt von

Daniel Bucher
Urbana, Illinois
Würzburg, 2008
Eingereicht am: .......................................................................................................................

Mitglieder der Promotionskommission:
Vorsitzender : Prof Dr. Müller .................................................................................................................
Gutachter : Prof Dr. Buchner.....................................................................................................................
Gutachter : Prof Dr. Nagel ........................................................................................................................

Tag des Promotionskolloquiums: .............................................................................................

Doktorurkunde ausgehändigt am: ............................................................................................for Frances, Rose, Russ, and Tony who helped make this possible,
my parents for their unfaltering love and support,
and lastly, for the people who make this all worthwhile...Table of Contents
1 Abstract 1
Zussammenfassung 3
2 Introduction
2.1 The Drosophila larval neuromuscular junction 5
2.1.1 Morphology and physiology of the muscle 6 & 7 synapse 5
2.1.2 Synaptic transmission at the Drosophila neuromuscular junction 9
2.2 Null mutants characterized at the Drosophila neuromuscular junction 13
1562.2.1 Sap-47 13
972.2.2 Synapsin 15
156 97 2.2.3 Sap-47 Synapsin double mutants 16
2.2.4 Serine-Arginine protein kinase 3 17
2.2.5 Löchrig 19
2.3 Expression of light activated proteins at the Drosophila neuromuscular junction 21
2.3.1 Channelrhodopsin-2 21
2.3.2 Photoactivated adenylate cyclase 22

3 Materials and Methods
3.1 The setup 24
3.1.1 The slab 24
3.1.2 Faraday cage 25
3.1.3 Peltier element 25
3.1.4 Optics and manipulators 25
3.1.5 Microelectrodes 26
3.1.6 Amplifier(s) 28
3.1.7 Accquistion 30
3.1.8 Analysis 32
3.2 Recording at the Drosophila neuromuscular junction 33
3.2.1 Solutions 33
3.2.2 Dissection 33
3.2.3 Recording techniques and criteria 34
3.3 Immunocytochemistry at the Drosophila neuromuscular junction 354 Results

156 4.1 Sap-47 36
97 4.2 Synapsin 41
156 97 4.3 Sap-47 Syn double mutants 43
4.4 Serine-Arginine protein kinase 3 45
4.5 Löchrig 48
4.6 Channelrhodopsin-2 51
4.7 Photoactivated adenylate cyclase 53
5 Discussion
156 5.1 The potential Sap-47 phenotype 61
5.2 Null mutant data 63
5.3 A possible Löchrig phenotype 65
5.4 Heterologous expression of Channelrhodopsin-2 and PAC at the neuromuscular junction 66
6 References 68
Appendix A: Fortran code for analysis of eEJP decay 84
Appendix B: Cirriculm Vitae 89
Appendix C: Publication list 93