Anti-inflammatory effects of Radix Gentianae Macrophyllae(Qinjiao), Rhizoma Coptidis(Huanglian) and Citri Unshiu Pericarpium(Wenzhou migan) in animal models

Kim Kyoung, Rhee Hae, Park Eun, Jung Kiwon, Jeon Hyo, Kim Ji-Hong, Yoo Hunseung, Han Chang-Kyun, Cho Yong-Baik, Ryu Chun, Yang Hyung, Yoo Myung

Description

KHU14, an ethanolic extract of Radix Gentianae Macrophyllae ( Qinjiao ), Rhizoma Coptidis ( Huanglian ) and Citri Unshiu Pericarpium ( Wenzhou migan ) was tested for its anti-inflammatory effects. Methods Three out of 20 herbs were found to have anti-inflammatory effects. The formulation of these herbs, i.e. KHU14 was tested for croton oil-induced ear edema, carrageenan-induced paw edema, acetic acid-induced capillary permeability, cotton pellet and delayed type hypersensitivity. Results KHU14 exhibited anti-inflammatory effects in animal models of acute and chronic inflammation. The anti-inflammatory activity of KHU14 observed was comparable to that of celecoxib. KHU14 inhibited the production of NO and PGE 2 in LPS/IFN-gamma-stimulated peritoneal macrophages, and reduced edema and the amount of infiltrated cells in animal models. Conclusion KHU14 exhibited anti-inflammatory effects as demonstrated in typical immunological tests for anti-inflammation in vitro and in vivo .

Informations

• Publié par: biomed
• Publié le: 01 janvier 2008
• Nombre de lectures: 26

Extrait

Chinese Medicine

BioMedCentral

Open Access Research Antiinflammatory effects ofRadix Gentianae Macrophyllae (Qinjiao),Rhizoma Coptidis(Huanglian) andCitri Unshiu Pericarpium (Wenzhou migan) in animal models 1 2 1 2 Kyoung Soo Kim* , Hae In Rhee , Eun Kyung Park , Kiwon Jung , 2 2 2 3 Hyo Jin Jeon , JiHong Kim , Hunseung Yoo , ChangKyun Han , Yong 4 5 6 7 Baik Cho , Chun Jeih Ryu , Hyung In Yang and Myung Chul Yoo*

1 Address: EastWest Bone and Joint Research Center, Kyung Hee University Neo Medical Hospital, 149 Sangildong, Gangdonggu, Seoul 134090, 2 South Korea, Life Science Research Center, SK Chemicals, 600 Jungja1dong, ChanganKu, SuwonSi, KyoungkiDo 440745, South Korea, 3 4 Central R&D, Ahngook Pharm., IuiDong Yeongtonggu, SuwonSi, KyoungkiDo, South Korea, Central Research Institute, Whan In Pharm., Co., 5 Ltd. IuiDong, Yeongtonggu, SuwonSi, KyoungkiDo, South Korea, Department of Bioscience and Biotechnology, Sejong University, Seoul 143 6 747, South Korea, Department of Internal Medicine, Kyung Hee University Neo Medical Hospital, 149 Sangildong, Gangdonggu, Seoul 134 7 090, South Korea and Department of Orthopedic Surgery, Kyung Hee University Neo Medical Hospital, 149 Sangildong, Gangdonggu, Seoul 134090, South Korea

Email: Kyoung Soo Kim*  [e-mail]; Hae In Rhee  [e-mail]; Eun Kyung Park  [e-mail]; Kiwon Jung  [e-mail]; Hyo Jin Jeon  [e-mail]; JiHong Kim  ji[e-mail]; Hunseung Yoo  [e-mail]; ChangKyun Han  ckhan@ahngook.com; YongBaik Cho  [e-mail]; Chun Jeih Ryu  [e-mail]; Hyung In Yang  [e-mail]; Myung Chul Yoo*  [e-mail] * Corresponding authors

Published: 2 September 2008 Received: 14 June 2007 Accepted: 2 September 2008 Chinese Medicine2008,3:10 doi:10.1186/17498546310 This article is available from: http://www.cmjournal.org/content/3/1/10 © 2008 Kim et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:KHU14, an ethanolic extract ofRadix Gentianae Macrophyllae(Qinjiao),Rhizoma Coptidis(Huanglian) andCitri Unshiu Pericarpium(Wenzhou migan) was tested for its anti inflammatory effects.

Methods:Three out of 20 herbs were found to have antiinflammatory effects. The formulation of these herbs, i.e. KHU14 was tested for croton oilinduced ear edema, carrageenaninduced paw edema, acetic acidinduced capillary permeability, cotton pellet and delayed type hypersensitivity.

Results:KHU14 exhibited antiinflammatory effects in animal models of acute and chronic inflammation. The antiinflammatory activity of KHU14 observed was comparable to that of celecoxib. KHU14 inhibited the production of NO and PGE in LPS/IFNgammastimulated 2 peritoneal macrophages, and reduced edema and the amount of infiltrated cells in animal models.

Conclusion:KHU14 exhibited antiinflammatory effects as demonstrated in typical immunological tests for antiinflammationin vitroandin vivo.

Background Herbal extracts from traditional Chinese medicine can be formulated to develop novel herbal medicines as potent

as synthetic medicines [13]. We investigatedin vitroanti inflammatory properties of 20 medicinal herbs used in Chinese medicine in order to develop a new herbal for

Page 1 of 7 (page number not for citation purposes)