The Truffle Project

Informations

• Publié par: NormanWozniak
• Publié le: 05 février 2016
• Nombre de lectures: 44
• Licence : Libre de droits
• Langue: English
• Poids de l'ouvrage: 9 Mo

Extrait

TRUFFLE PROJECT

Is there an other way?

PROJECT OverVIEW

Isolate 1 truffle microbes

PHASE 1

Identify them by 16s sequencing 2 & microscope observation

Cultivate 3 each one individually

Screen culture with and 4 without precursors in the culture

Cultivate microbes producing some truffle aromas

PROJECT OverVIEW

Sequence 1 the strain genome

PHASE 2 Analyse the Bioinformatic compounds analysis of 2 3 produced by metabolic GC-MS pathway

Scientific publication of the result?

PHASE 3 = Engineer a super-productive microbe

Test hypothesis by 4 generating knockout mutants

WHAT YOU WILL LEARN

How to 1 design an experiment How to identify a 5 microbe from its 16s DNA

Microbes cultivation and 2 isolation methods How to transform and 6 generate knockout mutants

If phase 1 & 2 are successful

How to design 3 and run a PCR Biochemistry 7 techniques to purify molecules

How to send DNA 4 samples for sequencing How to sequence and 8 analyse a whole genome

Become a true synthetic biologist

Step by 1 step learning

JOIN THE PROJECT

A GOOD WAY TO LEARN

Mentoring by 2 BosLab team

Join us, become a member!!!

Use of diverse 3 molecular biology techniques

No guaranty of s 4 l scienc success!! This i rea e