Tutorial 4—Lactating Cows In the CNCPSv4, lactation requirements are calculated independently from maintenance requirements. Lactation requirements are ranked third in nutrient priority for the animal (maintenance, pregnancy, lactation and growth). They are calculated as: ME required (Mcals/d) = Milk Production * (((Milk Fat * 0.0962) + 0.3512) / 0.644) MP required (gms/d) = (10 * Milk Production * Milk Protein) / 0.65 Where: milk production is kg/d Milk fat is % Milk protein is % True protein The 0.644 coefficient in the ME equation is the efficiency of use of ME to NE for lactation based upon the work of Moe et. al. The 0.65 coefficient in the MP equation is the efficiency of use of MP to net protein from the 1985 NRC. In other words, the model calculates the net energy and net protein required for the inputted milk production (and composition) and converts it to a metabolizable basis using these efficiency factors. Diet evaluation with the CNCPSv4 is accomplished by comparing required and supplied Mcals or grams rather than with concentrations of nutrients. The same is true when evaluating the amino acid adequacy of a diet. Amino acid content of tissue and milk protein used in the CNCPSv4 are as follows: Amino Acid Composition (g/100g) Amino Acid Tissue Milk protein Methionine 1.97 2.71 Lysine 6.37 7.62 Arginine 6.60 3.40 Threonine 3.90 3.72 Leucine 6.70 9.18 Isoleucine 2.84 5.79 Valine 4.03 5.89 Histidine 2.47 2.74 ...