Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces

Meyer Ulrich, Depprich Rita, Ommerborn Michelle, Zipprich Holger, Naujoks Christian, Handschel Jörg, Wiesmann Hans-Peter, Kübler, Meyer

Description

Osseointegration is crucial for the long-term success of dental implants and depends on the tissue reaction at the tissue-implant interface. Mechanical properties and biocompatibility make zirconia a suitable material for dental implants, although surface processings are still problematic. The aim of the present study was to compare osteoblast behavior on structured zirconia and titanium surfaces under standardized conditions. Methods The surface characteristics were determined by scanning electron microscopy (SEM). In primary bovine osteoblasts attachment kinetics, proliferation rate and synthesis of bone-associated proteins were tested on different surfaces. Results The results demonstrated that the proliferation rate of cells was significantly higher on zirconia surfaces than on titanium surfaces (p < 0.05; Student's t -test). In contrast, attachment and adhesion strength of the primary cells was significant higher on titanium surfaces (p < 0.05; U test). No significant differences were found in the synthesis of bone-specific proteins. Ultrastructural analysis revealed phenotypic features of osteoblast-like cells on both zirconia and titanium surfaces. Conclusion The study demonstrates distinct effects of the surface composition on osteoblasts in culture. Zirconia improves cell proliferation significantly during the first days of culture, but it does not improve attachment and adhesion strength. Both materials do not differ with respect to protein synthesis or ultrastructural appearance of osteoblasts. Zirconium oxide may therefore be a suitable material for dental implants.

Informations

• Publié par: biomed
• Publié le: 01 janvier 2008
• Nombre de lectures: 7
• Langue: English

Extrait

Head & Face Medicine

BioMedCentral

Open Access Research Behavior of osteoblastic cells cultured on titanium and structured zirconia surfaces 1 2 3 Rita Depprich , Michelle Ommerborn* , Holger Zipprich , †1 †1 4 Christian Naujoks , Jörg Handschel , HansPeter Wiesmann , 1 1 Norbert R Kübler and Ulrich Meyer

1 2 Address: Department of Cranio and Maxillofacial Surgery, HeinrichHeineUniversity, Düsseldorf, Germany, Department of Operative and 3 Preventive Dentistry and Endodontics, HeinrichHeineUniversity, Düsseldorf, Germany, Department of Prosthetic Dentistry, Section of Materials 4 Sciences, Johann Wolfgang Goethe University, Frankfurt, Germany and Department of Cranio and Maxillofacial Surgery, Westphalian Wilhelms University, Münster, Germany

Email: Rita Depprich  [e-mail]duesseldorf.de; Michelle Ommerborn*  [e-mail]duesseldorf.de; Holger Zipprich  [e-mail]frankfurt.de; Christian Naujoks  [e-mail]duesseldorf.de; Jörg Handschel  [e-mail]duesseldorf.de; HansPeter Wiesmann  [e-mail]; Norbert R Kübler  [e-mail]duesseldorf.de; Ulrich Meyer  [e-mail]duesseldorf.de * Corresponding author †Equal contributors

Published: 8 December 2008 Received: 27 October 2008 Accepted: 8 December 2008 Head & Face Medicine2008,4:29 doi:10.1186/1746160X429 This article is available from: http://www.headfacemed.com/content/4/1/29 © 2008 Depprich et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract Background:Osseointegration is crucial for the longterm success of dental implants and depends on the tissue reaction at the tissueimplant interface. Mechanical properties and biocompatibility make zirconia a suitable material for dental implants, although surface processings are still problematic. The aim of the present study was to compare osteoblast behavior on structured zirconia and titanium surfaces under standardized conditions. Methods:The surface characteristics were determined by scanning electron microscopy (SEM). In primary bovine osteoblasts attachment kinetics, proliferation rate and synthesis of bone associated proteins were tested on different surfaces. Results:The results demonstrated that the proliferation rate of cells was significantly higher on zirconia surfaces than on titanium surfaces (p < 0.05; Student'sttest). In contrast, attachment and adhesion strength of the primary cells was significant higher on titanium surfaces (p < 0.05;Utest). No significant differences were found in the synthesis of bonespecific proteins. Ultrastructural analysis revealed phenotypic features of osteoblastlike cells on both zirconia and titanium surfaces.

Conclusion:The study demonstrates distinct effects of the surface composition on osteoblasts in culture. Zirconia improves cell proliferation significantly during the first days of culture, but it does not improve attachment and adhesion strength. Both materials do not differ with respect to protein synthesis or ultrastructural appearance of osteoblasts. Zirconium oxide may therefore be a suitable material for dental implants.

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