Cell proliferation and cell survival in the dentate gyrus of adult mice under naturalistic conditions [Elektronische Ressource] / vorgelegt von Ariane Santoso

Ariane Santoso

Sujets

• Biologie

Informations

• Publié par: ludwig-maximilians-universitat_munchen
• Publié le: 01 janvier 2006
• Nombre de lectures: 25
• Langue: English
• Poids de l'ouvrage: 2 Mo

Extrait

Cell proliferation and cell survival
in the dentate gyrus of adult mice
under naturalistic conditions
Dissertation
an der Fakultät für Biologie
der Ludwig-Maximilans-Universität München

vorgelegt von
Ariane Santoso
München, Dezember 2006

1. Gutachter: Prof. Dr. York Winter
2. Gutachter: Prof. Dr. George Boyan

Tag der mündlichen Prüfung: 12. Oktober 2009

Table of Contents
Table of Contents
ABBREVIATIONS..................................................................................................................5
SUMMARY ..............................................................................................................................6
ZUSAMMENFASSUNG.........................................................................................................8
GENERAL INTRODUCTION.............................................................................................10
AIMS OF THIS THESIS ......................................................................................................18
CHAPTER 1 INDIVIDUALLY DOSED ORAL DRUG ADMINISTRATION TO
SOCIALLY-LIVING TRANSPONDER-TAGGED MICE BY A WATER
DISPENSER UNDER RFID CONTROL.............................................................................20
1 ABSTRACT...........................................................................................................................20
2 INTRODUCTION ...................................................................................................................21
3 METHODS............................................................................................................................22
4 RESULTS..............................................................................................................................25
5 DISCUSSION.........................................................................................................................28
6 REFERENCES.......................................................................................................................30
CHAPTER 2 NEUROGENESIS IN ADULT MICE IS NOT ENHANCED IN A
NATURALISTIC ENVIRONMENT BUT ONLY BY UNNATURAL
LOCOMOTION......................................................................................................................33
1 ABSTRACT...........................................................................................................................33
2 INTRODUCTION ...................................................................................................................34
3 MATERIALS AND METHODS ................................................................................................40
3.1 GENERAL METHODS.....................................................................................................40
3.2 EXPERIMENTAL DESIGN ...............................................................................................45
3.2.1 Simple environment and exploration and learning in a complex environment ...45
3.2.2 Locomotion types: wheel versus plane ................................................................48
3.2.3 Persistence of enhanced cell proliferation following running activity ................49
4 RESULTS..............................................................................................................................50
4.1 BEHAVIOR ...................................................................................................................50
4.2 BRDU-POSITIVE CELLS ................................................................................................53
5 DISCUSSION.........................................................................................................................56
5.1 CELL PROLIFERATION AND SURVIVAL ARE AFFECTED IN AN ENRICHED, BUT
NOT IN A NATURALISTIC ENVIRONMENT.......................................................................57
5.2 METHODICAL CONSIDERATIONS ..................................................................................63
5.3 CONCLUSIONS..............................................................................................................64
6 REFERENCES.......................................................................................................................65
CHAPTER 3 CELL PROLIFERATION AND BEHAVIOR: INSIGHTS.....................71
1 INTRODUCTION ...................................................................................................................71
2 EXPERIMENTS.....................................................................................................................73
3 Table of Contents
2.1 CELL PROLIFERATION IN THE NATURALISTIC ENVIRONMENT WITH BRDU
INJECTIONS ..................................................................................................................73
2.2 ORAL APPLICATION OF BRDU IN A NECTAR-FEEDING BAT (GLOSSOPHAGA
SORICINA)....................................................................................................................74
2.3 CLASSIFICATION AND DISTRIBUTION OF BRDU-LABELED CELLS..................................76
2.4 BEHAVIOR IN THE COMPLEX ENVIRONMENT ................................................................80
2.5 RECOVERY FROM A 7 HOUR SHIFT OF LIGHT-DARK CYCLE...........................................84
2.6 RUNNING IN WHEELS AND TUBES AD LIBITUM AND LIMITED TO 1000 M ......................86
2.7 PRESENCE OF AN IMMOBILIZED RUNNING WHEEL ........................................................88
3 DISCUSSION.........................................................................................................................89
4 REFERENCES.......................................................................................................................92
GENERAL DISCUSSION ....................................................................................................95
Oral BrdU administration is a suitable alternative to BrdU injections............................95
Cell proliferation in a naturalistic environment is not enhanced by exploration or
spatial learning ................................................................................................................95
Pro-proliferative effect of physical activity is transient and strongly depends on
activity level ....................................................................................................................97
Conclusion.......................................................................................................................98
Outlook............................................................................................................................98
References .......................................................................................................................99
APPENDICES ......................................................................................................................101
APPENDIX A METHODICAL DETAILS..................................................................................101
A.1 ANIMALS AND TREATMENTS......................................................................................101
A.2 CAGES .......................................................................................................................104
A.3 TREATMENTS AND PARAMETERS IN THE SIMPLE AND COMPLEX ENVIRONMENT.........109
A.4 THE HISTOLOGICAL PROCEDURE................................................................................113
A.5 QUANTIFICATION OF BRDU-LABELED CELLS.............................................................122
A.6 REFERENCES..............................................................................................................127
APPENDIX B FIGURES AND TABLES.....................................................................................128
B.1 INDEX OF FIGURES .....................................................................................................128
B.2 INDEX OF TABLES.......................................................................................................129
B.3 CONTENT OF CD-ROM .............................................................................................130
THANKS TO........................................................................................................................131
CURRICULUM VITAE......................................................................................................132

4 Abbreviations
Abbreviations
App Appendix
BrdU 5-bromo-2'-deoxyuridine
BW Body weight
CCD Charge-coupled device
dd Dorsodorsal blade of DG
dv Dorsoventral blade of DG
DG Dentate gyrus
EB Eye blink conditioning
GCL Granule cell layer
hi Hilus
IMA Integrated morphometry analysis
i.d. Inner diameter
i.p. Intraperitoneal
LD light-dark
MWM Morris water maze
n Sample size
o.d. Outer diameter
pAB Primary antibody
PIT Passive Integrated Transponder
PT Post-treatment time
RFID Radio frequency identification
RT Room temperature
sAB Secondary antibody
SD Standard deviation
SEM Standard error of the mean
SGZ Subgranular zone
ve Ventral DG
5 Summary
Summar