Chemical and biological investigations of Vietnamese cyanobacteria [Elektronische Ressource] / vorgeelgt von Le Thi Anh Tuyet
212 pages
Deutsch

Chemical and biological investigations of Vietnamese cyanobacteria [Elektronische Ressource] / vorgeelgt von Le Thi Anh Tuyet

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212 pages
Deutsch
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Ernst Moritz Arndt-Universität Greifswald Mathematisch-Naturwissenschaftliche Fakultät Chemical and Biological Investigations of Vietnamese Cyanobacteria Inauguraldissertation zur Erlangung des akademischen Grades doctor rerum naturalium (Dr. rer. nat.) an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald vorgelegt von Le Thi Anh Tuyet geboren am 19.05.1973 in Thanh Hoa, Vietnam Greifswald, Juli 2010 Dekan: Prof. Dr. Klaus Fesser……………………………………………… 1. Gutachter: Prof. Dr. Johannes F. Imhoff 2. Gutachter: PD. Dr. Sabine Mundt Tag der Promotion:……16.09.2010…………………………………………………………… Hiermit erkläre ich, daß diese Arbeit bisher von mir weder an der Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität Greifswald noch einer anderen wissenschaftlichen Einrichtung zum Zwecke der Promotion eingereicht wurde. Ferner erkläre ich, daß ich diese Arbeit selbständig verfaßt und keine anderen als die darin angegebenen Hilfsmittel benutzt habe.

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Publié par
Publié le 01 janvier 2010
Nombre de lectures 189
Langue Deutsch
Poids de l'ouvrage 7 Mo

Extrait




Ernst Moritz Arndt-Universität Greifswald
Mathematisch-Naturwissenschaftliche Fakultät




Chemical and Biological
Investigations of Vietnamese
Cyanobacteria

Inauguraldissertation
zur
Erlangung des akademischen Grades
doctor rerum naturalium (Dr. rer. nat.)
an der Mathematisch-Naturwissenschaftlichen Fakultät
der
Ernst-Moritz-Arndt-Universität Greifswald

vorgelegt von Le Thi Anh Tuyet
geboren am 19.05.1973
in Thanh Hoa, Vietnam







Greifswald, Juli 2010
























Dekan: Prof. Dr. Klaus Fesser………………………………………………

1. Gutachter:
Prof. Dr. Johannes F. Imhoff

2. Gutachter:
PD. Dr. Sabine Mundt

Tag der
Promotion:……16.09.2010……………………………………………………………






Hiermit erkläre ich, daß diese Arbeit bisher von mir weder an der
Mathematisch-Naturwissenschaftlichen Fakultät der Ernst-Moritz-Arndt-Universität
Greifswald noch einer anderen wissenschaftlichen Einrichtung zum Zwecke der
Promotion eingereicht wurde.

Ferner erkläre ich, daß ich diese Arbeit selbständig verfaßt und keine anderen
als die darin angegebenen Hilfsmittel benutzt habe.




Greifswald, den 19 Juli 2007 Unterschrift




Le Thi Anh Tuyet

a


.....................................................................................................1
1.1 Cyanobacteria.................................................................................................................. 1
1.1.1 Cyanobacterial physiology and morphology..................................................................... 1
1.1.2 Ecology of cyanobacteria................................................................................................ 2
1.1.3 Classification of cyanobacteria........................................................................................ 3
1.2 Cyanobacteria–a new and rich source of novel bioactive compounds with
pharmaceutical potential ........................................................................................................ 6
1.2.1 Antimicrobials.............................................................................................................. 9
1.2.2 Cytotoxic and antitumoural activities............................................................................. 12
1.2.3 Antiviral activity.......................................................................................................... 18
1.2.4 Toxins and other pharmacologically active compounds ................................................... 19
1.3 Aim of the work.............................................................................................................. 22


...........................................................................23
2.1 Biological materials....................................................................................................... 23
2.1.1 Soil cyanobacteria........................................................................................................ 23
2.1.2 Marine cyanobacteria ................................................................................................... 29
2.1.3 Bacteria, yeast, and cancer cell lines as test organisms .................................................... 31
2.2 Chemicals....................................................................................................................... 31
2.2.1 Cultivation of cyanobacteria ......................................................................................... 31
2.2.2 Cultivation of bacteria and yeast as test organisms.......................................................... 32
2.2.3 General laboratory chemicals.......................................................................................... 32
2.2.4 Chemical reagents........................................................................................................ 32
2.2.5 Fatty acid analysis ......................................................................................................... 33
2.3 Solvents............................................................................................................................ 33
2.4 Equipment in generally company, town, country ...................................................... 34
2.4.1 Cultivation of cyanobacteria ......................................................................................... 34
2.4.2 Extraction ..................................................................................................................... 34
2.4.3 Isolation of secondary metabolites................................................................................. 35
2.4.3.1 Thin layer chromatography (TLC).......................................................................... 35
2.4.3.2 Preparative TLC ................................................................................................... 35
2.4.3.3 Open column chromatography ............................................................................... 35
2.4.3.4 HPLC .................................................................................................................. 36
2.4.4 Agar plate diffusion test ................................................................................................. 36
2.4.5 Bioautographic TLC assay.............................................................................................. 37
2.4.6 Fatty acid analyses ....................................................................................................... 37
2. 5 Cultivation of cyanobacteria ....................................................................................... 38
2.5.1 The stock culture ......................................................................................................... 38
2.5.2 The batch culture ......................................................................................................... 38
2.5.3 The large scale culture.................................................................................................. 38
2.6 Extraction ....................................................................................................................... 39
2.6.1 Extraction of intracellular compounds............................................................................ 39
2.6.2 Extraction of extracellular compounds........................................................................... 40

2.7 Bioassays....................................................................................................................... 41
2.7.1 Assays for antimicrobial activity ................................................................................... 41
2.7.1.1 Agar diffusion assay.............................................................................................. 41
2.7.1.2 Bioautographic TLC assay..................................................................................... 42
2.7. 2 Assays for cytotoxic activity ........................................................................................ 43
2.8 Fractionation and isolation of the secondary metabolites of 6 cyanobacterial strains
................................................................................................................................................. 43
2.8.1 Fractionation and isolation of the secondary metabolites of Westiellopsis sp.VN................. 43
2.8.2 Fractionation and isolation of the secondary metabolites of Calothrix javanica ................. 45
2.8.3. Fractionation and isolation of the secondary metabolites of Scytonema ocellatum .............. 47
2.8.4 Fractionation and isolation of the secondary metabolites of Anabaena sp.......................... 49
2.8.5 Fractionation and isolation of the secondary metabolites of Nostoc sp. ............................. 50
2.8.6 Fractionation and isolation of the secondary metabolites of Lyngbya majuscula.................. 51
2.8.6.1 Method 1.............................................................................................................. 51
2.8.6.2 Method 2.............................................................................................................. 53
2.9 Structure elucidation of the isolated secondary metabolites................................... 55
2.9.1 Structure elucidation of compounds isolated from Westiellopsis sp.VN and Lyngbya majuscula
............................................................................................................................................. 55
2.9.2 Structure elucidation of compounds isolated from Calothrix javanica and Scytonema
ocellatum .........................................................

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