Improvements in the analysis of food contaminations deriving from packaging materials [Elektronische Ressource] / vorgelegt von Thorsten Rothenbacher

Thorsten Rothenbacher

Informations

• Publié par: universitat_hohenheim
• Publié le: 01 janvier 2009
• Nombre de lectures: 32
• Langue: English
• Poids de l'ouvrage: 1 Mo

Extrait

Improvements in the analysis
of food contaminations
deriving from packaging materials



Dissertation zur Erlangung des Doktorgrades
der Naturwissenschaften (Dr. rer. nat.)



Fakultät Naturwissenschaften
Universität Hohenheim



Institut für Lebensmittelchemie


vorgelegt von
Thorsten Rothenbacher


aus Konstanz
2009



















Die vorliegende Arbeit wurde am 03.12.2009 von der Fakultät Naturwissenschaften
der Universität Hohenheim als „Dissertation zur Erlangung des Grades eines Doktors
der Naturwissenschaften“ angenommen.

Die mündlichen Prüfung fand am 28.01.2010 mit folgenden Prüfern statt:

Dekan: Prof. Dr. H. Breer
Berichterstatter: 1. Prüfer: Prof. Dr. W. Schwack
2. Prüfer: Prof. Dr. H.-D. Isengard
3. Prüfer: Prof. Dr. W. Vetter

Preliminary Remarks

Parts of the work presented in this thesis have already been published or
submitted to international peer reviewed journals and/ or were presented on scientific
conferences as oral or poster presentations:

Full papers:
1. T. Rothenbacher, M. Baumann and D. Fuegel. 2-Isopropylthioxanthone (2-ITX) in
food and food packaging materials on the German market. Food Additives and
Contaminants 2007; 24: pp. 438-444
2. T. Rothenbacher, W. Schwack. Determination of epoxidized soybean oil by gas
chromatography/single quadrupole and tandem mass spectrometry stable isotope
dilution assay. Rapid Communications in Mass Spectrometry 2007; 21: pp. 1937-
1943
3. T. Rothenbacher, W. Schwack. Non-targeted multi-component analytical screening
of plastic food contact materials using fast interpretation of deliverables via expert
structure-activity relationship software Journal of AOAC INTERNATIONAL 2009;
*92 (3): pp. 941-950
4. T. Rothenbacher, W. Schwack. Rapid and nondestructive analysis of phthalic acid
esters in toys of poly(vinyl chloride) by direct analysis in real time–single
quadrupole mass spectrometry. Rapid Communications in Mass Spectrometry
2009; 23: pp. 2829–2835
5. T. Rothenbacher, W. Schwack. Rapid identification of additives in poly(vinyl
chloride) lid gaskets by direct analysis in real time ionisation and single-quadrupole
mass spectrometry. Rapid Communications in Mass Spectrometry 2010; 24: pp.
21-29

* Reprinted from The Journal of AOAC INTERNATIONAL, Rothenbacher, T., and Schwack, W. (2009)
J. AOAC Int. 92, 941. Copyright 2009 by AOAC INTERNATIONAL. Poster presentations:
1. “Schnelles Screening mit DART-MS am Beispiel von Weichmachern in PVC“.
Poster at the Deutscher Lebensmittelchemikertag 2009 (14.-16. Sept., Berlin) “
2. “Nachweis von Phthalsäureestern in Spielzeug und Babyartikeln aus PVC mit
DART™-MS in 4 min“. Poster at the Deutscher Lebensmittelchemikertag 2008
(08.-09. Sept., Kaiserslautern)
3. “Identifizierung von Additiven in Deckeldichtungen mit DART™-MS in 5 min?“.
Poster at Deutscher Lebensmittelchemikertag 2008 (08.-09. Sept., Kaiserslautern)
4. “Bestimmung von epoxidiertem Sojaöl mittels GC-MS(/MS) SIVA”. Poster at
Deutscher Lebensmittelchemikertag 2007 (10.–12. Sept., Nürnberg-Erlangen)

Oral presentations:
1. “Schnelle und zerstörungsfreie Analytik von Phthalsäureestern in Spielzeug und
Babyartikeln mittels DART-MS”. Presentation at ANAKON 2009 (17.-20. Mar.,
Berlin))
2. “Schnelles Screening für Weichmacher in Spielzeug und Deckeldichtungen aus
PVC mittels DART-MS“. Presentation at Tagung des Regionalverbandes Südwest
der Lebensmittelchemischen Gesellschaft 2009 (02.-03. Mar., Koblenz)
3. “Bestimmung des Weichmachers ESBO mittels GC/MS-SIVA“. Presentation at
Tagung des Regionalverbandes Südwest der Lebensmittelchemischen
Gesellschaft 2007 (05.-06. Mar., Gießen)

Contributions to the dissertation

All experimental and analytical work including analyses and interpretations of
all obtained data was performed by Thorsten Rothenbacher, except otherwise
mentioned in this section. Moreover, conceptions and the preparations of the original
versions of the manuscripts leading to publications originated from his initiative.
Professor Dr. W. Schwack was the supervisor of this work and helped to find
optimum formulations and forms for the English text manuscripts, except for paper 1.
In case of paper 1, samples selections, analysis strategy and the sample preparation
for food contact materials were developed by Thorsten Rothenbacher and sample
preparation in foods by Rüdiger Weishaar. Practical work was done by lab assistants
of the CVUA Stuttgart under supervision of Thorsten Rothenbacher, Dr. Markus
Baumann, Diane Fügel, and Rüdiger Weishaar. The publication text was from
Thorsten Rothenbacher with suggestions from Dr. Markus Baumann and Diane
Fügel. Sample preparation of 2-isopropylthioxanthone in foods and the spiking
procedure was from Diane Fügel, the instrumental analysis part was written by Dr.
Markus Baumann. For paper 2 the practical works according the sample preparation
and the solving of analytical standards in isooctane was carried out by lab assistants
of the CVUA Stuttgart under the supervision of Thorsten Rothenbacher. In case of
paper 3 practical work was made by lab assistants of the CVUA Stuttgart under the
supervision of Werner Altkofer and Thorsten Rothenbacher according sample
13preparation, preparation of C -labelled ethyl 9,10,12,13-diepoxyoctadecanoate and 18
13C -labelled methyl 9,10,12,13-diepoxyoctadecanoate. 18

Table of Contents

I. General Introduction........................................................................1
I.1. Constituents of plastic materials................................................................... 1
I.1.a. Polymers................................................................................................... 1
I.1.b. Non polymeric constituents....................................................................... 1
I.2. Regulation of plastic food contact materials by the European Union ........... 5
I.2.a. General regulations .................................................................................. 5
I.2.b. Regulations covering specific substances ................................................ 6
I.3. Substance transfer into food and its impact on human health...................... 7
I.3.a. Migration 7
I.3.b. Risk exposure........................................................................................... 9
I.4. Plastic food contact material analysis........................................................... 9
I.4.a. Targeted analysis ................................................................................... 10
I.4.b. Non targeted analysis............................................................................. 11
I.5. An actual example for alarming migrants deriving from one product.......... 13
I.6. Phthalic acid esters - a transferable problem for toys made of plastic
materials ............................................................................................................... 15
I.7. Aims of the study........................................................................................ 15
I.8. References................................................................................................. 16
II. Non-targeted multi-component analytical screening of plastic
food contact materials using fast interpretation of deliverables via
expert structure-activity relationship software. ................................31
II.1. Abstract...................................................................................................... 31
II.2. Introduction ................................................................................................ 32
II.3. Materials and methods............................................................................... 35
II.4. Results and discussion 38
II.5. Conclusion ................................................................................................. 49
II.6. Acknowledgment........................................................................................ 50
II.7. References 50 III. 2-Isopropylthioxanthone (2-ITX) in food and food packaging
materials on the German market ........................................................55
III.1. Abstract...................................................................................................... 55
III.2. Introduction ................................................................................................ 55
III.3. Experimental .............................................................................................. 58
III.4. Results and discussion............................................................................... 61
III.5. Conclusion ................................................................................................. 65
III.6. Acknowledgement.....................................................................................